Effect of 2, 3-butanedione monoxime (BDM) on myocardial protection in cold cardioplegia

AIM: The effects of BDM on isolated rat heart in cold cardioplegia were studied. METHODS: Rat heart were subjected to cold cardioplegia at 4℃ for 8, 18 and 24 h.Then each heart was perfused (90 cm H₂O) in Langendorff model at 37℃ for 40 min. In the high K⁺ group(n=24) the hearts were preserved in St.Thomas cardioplegic solution, in BDM group(n=24) hearts were preserved in K-H solution with BDM 30 mmoL/L. RESULTS: After 18 h, heart rate and the coronary flow in BDM group were significantly higher than in high K⁺ group(P＜0.05). Activity of Na⁺-K⁺-ATPase in cell membrane and in mitochondrial membrane in the BDM group was significantly higher than high K⁺ group(P＜0.01). After 24 h, all hearts in high K⁺ group were dead, but were alive in BDM group. CONCLUSION: Under given experiment conditions, BDM did enhance the tolerance to cold ischemia significantly. The results showed that BDM may become a useful agent for prolong the storage period of heart in cold cardioglegia.     

Experimental research on cardiac fibrosis induced by recombinant mouse interleukin-11 in mice

AIM To observe the effect of recombinant mouse interleukin-11 （rmIL-11）injected subcutaneously into mice on heart structure and function and to determine its pro-fibrotic effect. METHODS C57BL/6 mice were randomly divided into experimental group and control group.The mice in experimental group were injected subcutaneously with recombinant mouse IL-11 at the dose of 100 μg·kg^-1·d^-1 for 3 consecutive weeks, while the control group were given equal volume of normal saline in the same way. After the experiment was finished, the parameters of heart function were measured by echocardiography.The heart weight was weighed and the cardiac weight index (CWI) was calculated. HE staining and Masson's trichrome staining were performed to observe the pathological changes and the extent of myocardial fibrosis in mouse myocardia respectively, and the cardiac collagen volume fraction (CVF) was calculated. The expression levels of extracellular matrix proteins in the myocardial tissues of mice, including type Ⅰ collagen, type Ⅲ collagen and fibronectin, were determined by Western blot. RESULTS Left ventricular ejection fraction and left ventricular fraction shortening in experimental group were obviously lower than those in control group (P<0.01), however left ventricular end-diastolic diamension and left ventricular end systolic dimension were significantly higher than those in control group (P<0.05).Compared with control group, the CWI was increased (P<0.01), the myocardial arrangement was disorder, the necrosis of cardiac myocytes was increased, and excessive deposition of collagen was observed in the myocardial tissues in experimental group. Correspondingly, the CVF and protein levels of type Ⅰ collagen, type Ⅲ collagen and fibronectin in the left ventricle in experimental group were increased significantly (P<0.05). CONCLUSION Injection of rmIL-11 into the mice subcutaneously induces fibrogenesis in the heart, which implies that IL-11 is likely a novel pro-fibrotic factor.     

利用SNP芯片对部分黄淮海玉米自交系遗传多样性分析

利用4 434个SNP标记对47份玉米自交系进行种质遗传多样性和杂种优势类群研究。采用Admixture软件对自交系的遗传结构进行分析,采用Treebest软件对自交系进行聚类分析和类群划分。结果表明,47份自交系分为6个类群,分别为瑞德群、兰卡斯特群、塘四平头群、PB群、旅大红骨群和自330亚群。10个自选系组配的杂交种主要利用的杂种优势模式为PB×塘四平头和瑞德×塘四平头,均为近年来黄淮海地区主要利用的杂种优势模式。     

液体肥料施用装置设计与试验

针对液体肥料发展迅速和液体肥料施用装置发展不完善的现状,研制了一种可测量液体肥施用装置主要技术性能及参数的试验台,在此基础上对喷头流量影响因素作了三因素二次回归正交旋转试验,得出了液体肥料质量分数、电动机转速及喷头口直径大小对喷头流量的影响规律及主次关系。     

表面式永磁同步电机电动助力转向控制原理分析

设计的电动助力转向表面式永磁同步电机变频调速系统由永磁同步电机、逆变器、转子位置检测装置和控制单元构成。表面式永磁同步电机电动助力转向系统的控制包括扭矩控制和矢量控制。扭矩控制是表面式永磁同步电机电动助力转向系统控制目标,矢量控制是对表面式永磁同步电机定子电流矢量的控制。阐明了表面式永磁同步电机电动助力转向系统的控制过程,分析了表面式永磁同步电机电动助力转向控制系统的助力特性设置、坐标变换和脉宽调制技术。     

武威某奶牛场犊牛及职工球虫病感染情况调查

为摸清该奶牛场的球虫感染情况,在甘肃省武威市某集约化牛场随机抽10头犊牛粪便及与犊牛直接接触的一线职工10人的粪便为样本,采用麦克马斯特计数方法,检测球虫病的感染情况。结果显示：该牛场球虫总感染率为80%,但OPG均小于5000,属于亚临床型球虫感染,该奶牛场职工没有球虫病感染情况。通过对球虫病感染的检查,诣在为该场犊牛球虫病的防控治疗提供依据。     

集雨农业雨水利用转化效率及其提高途径分析

集雨农业是指收集、存储雨水径流并将其作为农村家庭人畜饮用水、环境卫生用水和农业补充灌溉用水的农业生产活动,包括收集、存储、输送、利用等多个过程,但目前各地的集雨农业技术研究多注重单项技术研究,而没有把它们当作一个整体来进行研究,导致许多地方出现收集与存储、收集与利用、存储与输送、存储与利用等脱节的现象,使各个环节的雨水利用转化效率普遍偏低。本文首先比较系统完整地提出了集雨农业雨水利用转化效率的概念,将集雨农业的雨水收集、雨水存储、雨水输送和雨水利用等各个过程看作一个整体进行研究分析,然后结合有关试验资料提出了提高集雨农业雨水利用转化效率的途径。     

改进FPAR的煤矿区植被NPP损失研究

基于遥感的CASA模型是测算植被NPP的主要方法之一,当前CASA模型中普遍以月内某一时刻或几个时刻的植被NDVI值计算光合有效辐射吸收比例(FPAR),这一方法忽略了月内FPAR的整体性和连续性,在计算中会产生较大误差.文中以煤矿区为例,首先通过时空自适应反射率融合模型构建高时空分辨率的矿区遥感影像,改进CASA模型中FPAR的计算方法,以FPAR连续函数均值代表月FPAR,并评价改进效果.通过FPAR损失量测算各植被NPP变化量,为煤矿区生态环境变化做出科学的判断.     

蚯蚓粪与化肥配施对红小豆生长与品质的影响

为探究蚯蚓粪与化肥配施对陕西关中地区红小豆产量及品质的影响,以红小豆新品种保红947为研究对象,设置T1(纯化肥)、T2(20％蚯蚓粪+80％化肥)、T3(50％蚯蚓粪+50％化肥)、T4(80％蚯蚓粪+20％化肥)和CK(不施肥)5个处理,研究了不同处理下红小豆成熟期农艺性状、产量性状及品质性状的差异.结果表明:蚯蚓...     

绿洲生态水权界定及其分配

从水资源产权的角度来理解绿洲生态用水,期望为绿洲生态和环境的保护与恢复提供新的研究思路。首先对生态用水概念进行了辨析,给出了绿洲生态用水的概念;其次对绿洲生态水权从质和量两个方面进行了界定;最后提出绿洲生态水权分配的层次结构,介绍了绿洲生态用水的计算方法,重点对绿洲生态水权进行分配。从而进一步明晰了绿洲生态水权,为绿洲生态与环境的保护和恢复提供了新的理念和思路。