EZH2-mediated regulation of NF-κB target gene expression in gastric cancer

AIM: To explore the mechanism by which over-expression of enhancer of zeste homolog 2 (EZH2) in a panel of gastric cancer cell lines is involved in tumorigenesis of gastric cancer. METHODS: Real-time PCR and Western blot were employed to examine the mRNA and protein levels of EZH2, respectively. MTS assay, cell migration and soft agar assay were performed to investigate the role of EZH2 in the regulation of stomach cancer behaviors. The effect of EZH2 on NF-κB target gene expression was determined by Luciferase reporter and real-time PCR. Co-immunoprecipitation was used to analyze the interaction of EZH2 and p65 in HEK293T cells. RESULTS: The expression levels of EZH2 were significantly increased in the gastric cancer cells compared with normal gastric epithelial cells. Pharmacological inhibition by DZNep or knockdown of EZH2 significantly compromised AGS and SNU-16 cell activity, cell migration and anchorage-independent cell growth. Moreover, siRNA knockdown of EZH2 impaired NF-κB downstream targets, such as IL-8, CXCL5 and CCL20. In addition, the interaction of EZH2 and p65 was detected. CONCLUSION: EZH2 mediates the growth of gastric cancer cells through the regulation of NF-κB downstream gene expression.     

Impact of fluxapyroxad on the microbial community structure and functional diversity in the silty-loam soil

The aim of this work was to assess the effect of applying three different doses of fluxapyroxad on microbial activity, community structure and functional diversity as measured by respiration, microbial biomass C, phospholipid fatty acid(PLFA) and community-level physiological profiles(CLPPs). Our results demonstrated that substrate-induced respiration(on day 15) and microbial biomass C(on days 7 and 15) were inhibited by fluxapyroxad, but stimulation was observed thereafter. In contrast, fluxapyroxad addition increased the basal respiration and metabolic quotients(q CO2) and respiratory quotients(QR). Analysis of the PLFA profiles revealed that the total and bacterial biomass(both Gram-positive bacteria(GP) and Gram-negative bacteria(GN)) were decreased within the initial 15 days, whereas those as well as the GN/GP ratio were increased at days 30 and 60. F luxapyroxad input decreased the fungi biomass but increased the bacteria/fungi ratio at all incubation time. Moreover, high fluxapyroxad input(75 mg fluxapyroxad kg–1 soil dry weight) increased the microbial stress level. A principal component analysis(PCA) of the PLFAs revealed that fluxapyroxad treatment s ignificantly shifted the microbial community structure, but all of the observed effects were transient. Biolog results showed that average well color development(AWCD) and functional diversity index(H′) were increased only on day 60. I n addition, the dissipation of fluxapyroxad was slow in soil, and the degradation half-lives varied from 158 to 385 days depending on the concentration tested.     

沟槽滤棒压降影响因素的因子分析

[目的]深入研究沟槽滤棒特征参数及组成材料对滤棒压降的影响。[方法]选取常见的8种不同规格的沟槽滤棒及其主要组成材料为分析对象,采用相关性分析与因子分析,开展9个主要特征参数对沟槽滤棒压降的影响程度研究。[结果]试验表明,影响沟槽滤棒压降的特征参数间存在显著或极显著的相关性,相关系数矩阵中特征值大于1.0的前3个成分的累计贡献率达87.502%;由沟槽滤棒压降影响特征参数的旋转因子载荷矩阵得到3个公因子,总结定义后分别是:丝束总质量因子、单丝线密度/纤维素纸物理特性因子、沟槽特征参数因子。[结论]因子分析得出的公因子影响情况与实际沟槽滤棒生产检验过程中出现的情况基本吻合,进一步证实了沟槽滤棒特征参数对滤棒压降影响的准确性。     

Spectrum-Effect Relationship Between High Performance Liquid Chromatography Fingerprints and Anticoccidial Activities of a Compound Chinese Medicine

Quality control and screening of active substances in traditional Chinese medicines have been performed using fingerprint analysis. The spectrum-effect relationship between chromatography fingerprints and efficacy of herbal drugs is considered as a potentially useful method for determining active ingredients in complex mixtures. The study was designed to develop a method for determining the bioactive components of a compound Chinese medicine called Tiefeng based on spectrum-effect relationships between high-performance liquid chromatography （HPLC） fingerprints and anticoccidial activities. Four peaks of the established HPLC fingerprint indicate the main bioactive components of this medicine. In addition, pharrnacodynamic atlas was defined and used to assess the anticoccidial activity of Tiefeng from different sources for the first time. We found that the level of anticoccidial activity of Tiefeng was consistent with the degree of similarity between the pharmacodynamic atlas and chromatogram of any sample. Furthermore, effect of this medicine was related with the main active constituents, along with the origin and the harvesting time.     

安徽省农民“退宅进城入镇”的实践与成效——以天长市铜城镇为例

天长市铜城镇根据当地工业基础较好、农村"空巢户"较多、农民进城入镇的愿望较强烈的现状,通过调查研究,在集中民意的基础上规范操作,开展试点,循序渐进,创造条件,引导农民"退宅进城入镇",并积极引导农村土地流转,解除"退宅进城入镇"农民的后顾之忧。通过开展"退宅进城入镇"工作,为开发土地资源,增加经济效益,促进农地规范流转及农业现代化发展,助推城镇化进程,提高居民的生活质量探索了新道路。     

网络时代高校图书馆信息资源建设研究

信息技术给高校图书馆的发展带来了机遇和挑战,信息资源已经成为高校图书馆馆藏资源的重要组成部分。从理论、观念、服务模式等方面阐述了信息时代高校图书馆信息资源建设应采取的措施。     

浅谈大田县政策性水稻种植保险现状及对策

大田县开展政策性水稻保险工作取得显著的成效。该文总结了大田县推进政策性水稻种植保险的意义、现状、存在问题,并提出今后完善政策性水稻种植保险的对策。     

几种杀虫剂防治桃蛀螟试验

通过对桃树喷药,比较两个不同时期施用4种不同药剂的防治效果。结果表明,在产卵期喷施的4种药剂都有防蛀效果,甲维盐与灭幼脲混配的药效期更长;幼虫出现后喷药效果较差,因幼虫蛀入桃果内,药物难以发挥作用。     

杉木种子园下套种三叶青的块根产量分析

该试验研究了杉木种子园不同坡位套种三叶青,以及三叶青不同栽植方式的块根产量,结果表明,下坡的平均单株块根产量和单位面积块根产量均最高,中坡表现也比较好,上坡最低。相对于上坡,下坡的平均单株块根产量提高145.0%,单位面积块根产量提高203.7%,保存率增加15%。相对于裸根栽植,营养网袋栽植的三叶青平均单株块根产量提高59.3%,单位面积块根产量提高103.8%,保存率增加18%。     

氧化铝蒸发过程的机理建模与仿真

针对氧化铝蒸发工序中出口浓度检测滞后的现状，建立氧化铝生产逆流多效蒸发的动态机理模型。采用常微分方程组的MATLAB工具箱函数解法，并对第五、六效蒸发过程进行仿真研究。结果表明，该模型能够较好地逼近实际系统，并对出口浓度的在线实时监测有现实的指导意义。