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应用于作物体细胞杂种鉴定的遗传标记方法 总被引:1,自引:0,他引:1
着重介绍了4类遗传标记方法的原理及特点,简要概述了其在作物体细胞杂种鉴定方面的应用进展. 相似文献
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HUANG Kai-hong WANG Ling-yun ZHAO Xiao-long LIU Jian- hua CHEN Ru-fu ZHU Zhao-hua 《园艺学报》2007,23(2):258-261
AIM:The characteristics of nanometer particles, which were prepared by the conjugation of anti-VEGF monoclonal antibodies and 5-fluorouracil-loaded polylactic acid nanometer particles (5-FU-NPs), were investigated for improving the anticancer activity of 5-FU. METHODS:The method of couple linkage of chemical bonds was used to prepare the 5-FU-NPs with VEGF antibody, then the appearance, distribution of particle diameter, releasing in vitro and the immunological activity were detected. RESULTS:The 5-FU-Ab-NPs appeared as regular globular, the average particle diameter was (202±23)nm. The 5-FU-Ab-NPs possessed the similar delayed release character of 5-FUs. More than 80% of the immmunogical activity were detected in conjugates-retained antibody by the immunological methods and electron microscopy. CONCLUSION:The 5-FU-Ab-NPs possess the similar delayed released activity to 5-FU-NPs and have double activity of immune targeting and delayed releasing, which may increase the local concentration of 5-FU. 相似文献
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AIM:To investigate the mechanisms by which bilirubin inhibits acute lung injury (ALI). METHODS:30 female Wistar rats were divided into normal group, ALI group and bilirubin treatment group. ALI was induced by intravenous injection of LPS. The contents of OH-, H2O2 and O2· in the lung as well as the expression of caspase-3 in the lungs were investigated. RESULTS:(1) The contents of OH-, H2O2 and O2· in the lung homogenate and the expression of caspase-3 in the lungs in ALI group increased compared with those in normal group (P<0.05). (2) The contents of OH-, H2O2 and O2· in the lung homogenate and the expression of caspase-3 in the lungs in bilirubin treatment group increased compared with those in normal group, but decreased compared with those in ALI models (P<0.05). CONCLUSION:(1) Bilirubin was shown to be able to ameliorate apoptosis in ALI rats. (2) The increase in the contents of OH-, H2O2, O2· in ALI group indicated the development of oxidative lung injury, which was ameliorated by bilirubin. (3) Expression of caspase-3 confirmed that the model made by LPS was associated with apoptosis, which was reduced by bilirubin. 相似文献
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LIU Ya-wei LIU Jing-hua XIE Ru-jia LI Zhi-jie WANG Guo-jun HUANG Shao TANG Jing ZHAO Ming-zhe SUN Xue-gang DENG Peng JIANG Yong 《园艺学报》2007,23(10):1937-1941
AIM: To study the effect of human FAT10 on the apopotosis of HEK293 cells using flag-tagged human FAT10 protein.METHODS: The fragment of FAT10 gene was cloned into the pcDNA3-flag vector,which was identified by PCR,enzyme digestion and sequencing.The reconstructed plasmids were transfected into HEK293 cells.The expression of introduced FAT10 in the normal cultured and starved cells was detected respectively by Western blotting.XTT assay and DNA ladder method were used to analyze the effect of FAT10 on the apoptosis of starved HEK293 cells.RESULTS: The reconstructed plasmids were highly expressed in HEK293 cells with different expression mode at the mormal cultured and starved state.The livability of starved FAT10 overexpressed HEK293 cells was significantly lower than that of normal cultured cells.DNA ladder was observed in the starved FAT10 overexpressed cells,but not in the normal cultured cells.CONCLUSION: The eukaryotic expressed plasmids of flag-tagged FAT10 were constructed successfully,and highly expressed in HEK293.Overexpressed FAT10 enhances the apoptosis in the starved HEK293 cells. 相似文献
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本文试图澄清内蒙古究竟有多少种景天属植物,这些植物在内蒙古的生态分布情况。我们应用形态—地理分类的方法,对内蒙古景天属Sedum植物进行认真研究。研究认为:《内蒙古植物志》第二版记载的北景天(Sedum kamtschaticum Fisch.)和兴安景天(Sedum hsinganicumChu.)应为费菜;确定了兴安景天(Sedum hsinganicum Chu.)和费菜(Phdeimusaizoon L.)为温带亚洲分布种;阔叶景天(Sedum roborowskii Maxim.)为青藏高原东部分布种;藓状景天(Sedum polytrichoides Hemsl.)为中国-日本分布种;内蒙古景天属植物从东部渗入了1种东亚成分,从西南部渗入了1种青藏高原成分。 相似文献