Significance of expression of extracellular matrix metalloproteinase inducer and endothelin converting enzymes in lymphoid metastasis and prognosis in non-small-cell lung carcinoma

AIM: To investigate the expression of extracellular matrix metalloproteinase inducer (EMMPRIN) and endothelin converting enzymes (ECE) in non-small-cell lung carcinoma (NSCLC), and their relationship with lymphoid metastasis and prognosis.METHODS: Expression of EMMPRIN and ECE in 77 cases of patients with NSCLC was detected immunohistochemically.The relationship of the expression of EMMPRIN and ECE with tumor size, histological type, differentiation, lymphoid metastasis, clinical stage, and prognosis were analyzed.RESULTS: The expressive rate of EMMPRIN and ECE was 66% and 45%, respectively.The expression of EMMPRIN and ECE was associated positively with lymphoid metastasis (both P<0.01, r=0.371 and 0.467, respectively), and inversely with survival time (P<0.01 and P<0.01).No relationship was found in the expression of EMMPRIN and ECE with tumor size, histological type, and differentiation (P>0.05).The expression of EMMPRIN was associated with the expression of HGF in NSCLC (P<0.05, r=0.243).CONCLUSION: The expression of EMMPRIN and ECE is associated with lymphoid metastasis and prognosis in NSCLC.Overexpression of EMMPRIN and ECE implies infavourable prognosis in NSCLC.     

鸡的铜绿假单胞菌病的研究

１９９８年６－１１月天津武清,蓟县,河北玉田等地出现一种以眼睑肿胀,流泪,瞎眼及喙端,眼,践爪结痂为特征的鸡传染病。病鸡主要为３０－６０日龄。经鼻感染３０日龄鸡复制出了与自然病例相同的临床表现,并分离到接种菌。对分离菌的系统鉴定表明所分离的４株菌均为铜绿假单胞菌。     

Changes of PKAC-β, c-Fos and BDNF in cerebral cortex after intracerebral hemorrhage in rats treated with WIN55-212-2

AIM: To observe the effect of cannabinoid receptor (CB1R) agonist WIN55-212-2 on the expression of brain-derived neurotrophic factor (BDNF), c-Fos and protein kinase A beta-catalytic subunit (PKAC-β) in cerebrum cortex after intracerebral hemorrhage (ICH) in rats. METHODS: The intracerebral hemorrhage model of rat was made by the injection of collagenase Ⅶ, and WIN55-212-2 was intraperitoneally (ip) injected 30 min later. The rats were killed for sampling the brain tissues as specimens 24 h after ICH. The methods of immunohistochemical analysis and Western blotting were adopted to detect the expression of PKAC-β and BDNF. The mRNA expression of PKAC-β, c-Fos and BDNF was determined by RT-PCR. RESULTS: WIN55-212-2 obviously improved some nervous deficit symptoms and increased the expression of BDNF at mRNA and protein levels with upregulating the mRNA expression of c-Fos and downregulating the expression of PKA at mRNA and protein levels in the ipsilateral cerebral cortex. The proteins of PKAC-β, c-Fos and BDNF were expressed on the membrane or nucleus of the neuron or in the cytoplasm of glial cells, respectively. CONCLUSION: The expression of BDNF is induced not only by upregulation of c-Fos, but also by downregulation of PKA in WIN55-212-2 treated rats.     

免耕稻油轮作模式探讨

稻油轮作是保障我国粮油安全的重要耕作制度.从农业节肥减药、生态高效、机械化轻简栽培等视角出发,提出了适合湖南地区的免耕稻油轮作模式,阐述了关键技术,分析其可行性,并对其可持续发展进行展望.     

马铃薯杂交无性一代植物学性状与产量相关性的研究

马铃薯个体产量由其遗传性所约,并受生长发育过程中所经受的外界环境的影响^[1]。本研究以大西洋X白引4号、威芋3号X白引3号两个杂交组合无性一代混选的单薯系作研究对象,从地上部主要性状与产量、地下结薯状况与产量、地上主茎数与地下块茎的关系等方面的测定数据进行统计分析。结果：马铃薯的产量与单株的主茎数、结薯数、薯块大度、分枝数、茎叶重和茎粗等均呈正相关,而与株高的相关性不显著或不稳定。因此,能获得丰收的马铃薯个体的地上部应具备的性状是：植株有较多的主茎（3-5）或适当的分枝;生长健壮、茎粗叶茂、株高适宜的地上形态结构,这样就可达到地下部结薯多、大中薯比例高,产量也高。     

J亚群白血病病理学观察及抗原定位

通过病理组织学和免疫组织化学技 术对1日龄感染鸡定期进行病理组织学观察 及gp85病毒糖蛋白检测,确定病理变化和J 亚群白血病的组织学嗜性及二者的关系。结 果在3周龄时出现第1只发病鸡,以后所有 鸡发病且病变随着日龄的增加而加重,主要 在骨髓、肝脏、脾脏、肾脏、卵巢、心肌等组织 中出现瘤细胞增生,并有炎性反应;免疫组在 3周龄时,肝脏、心脏、肺脏、小肠、肾脏、卵巢、 骨骼肌开始出现阳性反应,抗原主要在胞浆 中出现,呈深蓝色颗粒。在10周龄～14周龄 时,骨髓呈中度的阳性反应,而其它组织的阳 性信号出现的程度未见大的变化;在23周龄 时,肝内肿瘤呈较强阳性反应,信号主要集中 在细胞核;整个实验过程中,法氏囊、脑始终 呈阴性反应。研究发现,骨髓及肿瘤的抗原 性较强,并且肿瘤的抗原性要强于骨髓;另外 还发现,在阳性反应强的组织中,病变较明 显,肿瘤细胞增生明显,即阳性反应的组织器 官易形成肿瘤转移灶。     

高效农业的绩效化评价与研究

发展高效农业不仅是解决我国人多地少、农民增产不增收的重要途径,而且也是实现传统农业改造,向现代化农业过度的基本要求.文章依据多目标综合评价方法的原理制订了高效农业绩效综合评价框架,利用自建的模型对寿光和杨凌示范区高效农业绩效进行了评价.     

沙漠增温效应下绿洲棉花热量生产潜力分析

在新疆干旱区,由于暖季沙漠增温效应的影响,与沙漠相邻绿洲区的热量资源相对优越,为植棉提供了有利条件。但随着时空变化,绿洲棉花热量生产潜力也呈现出相应的分异规律。本文以新疆北疆玛河流域石河子平原绿洲区为例,将绿洲与沙漠联系起来考虑,分析了距沙漠不同距离梯度的绿洲区棉花热量生产潜力。结果表明:石河子平原绿洲区内,距离沙漠最近区与最远区之间棉花热量生产潜力相差389Kg/hm2,绿洲与沙漠的距离平均每增加10Km,热量生产潜力下降43Kg/hm2。暖季沙漠增温效应对绿洲棉花的热量生产潜力影响明显,沙漠对相邻绿洲区的农业生产有着正面影响,起到了除太阳外第二个热量源的作用。