耐氟喹诺酮类药物大肠埃希氏菌基因突变的MAMA-PCR检测

采用MAMA-PCR法对72株已知突变菌株的突变情况进行了检测。结果，有2种gyrA83位突变：TCG→TTG和TCG缺失；4种gyrA87位突变：GAC→AAC、GAC→GGC、GAC→TAC和GAC→CAC；1种parC80位突变：AGC→ATC以及4种parC84位突变：GAA→AAA、GAA→GCA、GAA→GGA和GAA→GTA。采用同样的方法，对38株未知突变菌株的检测结果显示，gyrA83、gyrA87、parC 80和parC84位的突变检出的正确率分别为97．4％、94．7％、81．6％和94．7％。结果表明，MAMA-PCR有望成为监测耐氟喹诺酮类药物大肠埃希氏菌的分子生物学常规方法。     

深化闽台农业合作的战略思考

本文通过分析闽台农业合作发展现状与存在的问题,对其战略地位进行再认识,从而提出闽台农业合作战略转型与策略谋划的新思考,以期为持续发挥福建在海峡两岸农业合作中的引领作用,实现闽台农业合作更高层次的双赢,进一步提升福建现代农业综合竞争力,为海峡西岸社会主义新农村建设提供决策参考。     

铁皮石斛的特征特性及大棚栽培新技术

本文通过介绍铁皮石斛的特征特性及从大棚建设技术、移栽、栽培料处理、种植管理、病虫害管理等方面总结其大棚栽培技术要点,为生产上进一步推广应用提供参考.     

An unusual and novel heterozygous TCIRG1 mutation causes infantile malignant osteopetrosis

AIM: To investigate the underlying genetic changes of a Chinese patient with infantile malignant osteopetrosis (IMO). IMO is a monogenic disease, mostly caused by mutations of TCIRG1 and CLCN7 genes. The former is believed a homozygous gene and only cause the disease in homozygous or compound heterozygous status. However, it has been reported that heterozygous mutations also cause the disease in 6 non-Chinese cases. METHODS: Genomic DNA was extracted from peripheral blood of the patient and his parents. All exons and splice sites of TCIRG1 and CLCN7 genes were amplified by PCR followed by Sanger sequencing. Mutation detection in the 2 genes was also investigated in the parents. Haplotypes were constructed by variations obtained in mutation detection and microsatillites flanking TCIRG1 gene in the family by Cyrillic. Chromosomal microarray analysis (CMA) was performed to detect copy number variations (CNV) of the patient and his mother. RESULTS: A novel mutation c.449_452delAGAG (p.Gln149Glnfs16) was detected in the patient. This mutation truncated 666 amino acids at the C terminal of the V-ATPase 116 kD isoform a3 protein. It wiped out the entire ATPase V0 complex and was predicted to result in total loss of protein function. This mutation was also detected in the patient's father. No pathogenic mutation was detected in CLCN7 gene. CMA did not reveal any CNV involving TCIRG1 or CLCN7 gene. CONCLUSION: We reported a novel heterozygous mutation of TCIRG1 gene causing IMO. This represents the first IMO case in China caused by heterozygous TCIRG1 gene mutation.     

Effects of microglia-derived IL-1β on differentiation of OPCs in corpus callosum of septic neonatal rats

AIM: To explore whether IL-1β inhibits the oligodendrocyte precursor cell (OPCs) differentiation and affects axonal myelination. METHODS: One-day-old SD rats were randomly divided into control group and LPS group (48 rats in each group). The rats in LPS group were intraperitoneally injected with 1 mg/kg LPS. The rats in control group were injected with an equal volume of PBS. The rats in each group were further divided into 3 h, 24 h, 3 d, 7 d, 14 d and 28 d subgroups after injection. The expression of IL-1β and IL-1R₁ in the rat corpus callosum at 3 h, 24 h, 3 d, 7 d was determined by double immunofluorescence and Western blotting. The myelin basic protein(MBP) expression in the rat corpus callosum at 14 d, 28 d after injection was also measured. In vitro, primary OPCs culture was performed and divided into control group, 30 μg/L IL-1β group, 30 μg/L IL-1β+IL-1Ra group and 30 μg/L IL-1Ra group. The expression of MBP in the OPCs induced differentiation for 3 d was observed by double immunofluorescence and Western blotting. RESULTS: The expression of IL-1β and IL-1R₁ in the rat corpus callosum at 3 h, 24 h, 3 d, 7 d after LPS injection was obviously increased and the expression of MBP in the rat corpus callosum at 14 d, 28 d in LPS group was obviously decreased compared with control group in vivo. The level of MBP was significantly decreased after IL-1β treatment for 3 d in vitro. However, IL-1Ra (IL-1R inhibitor) reversed the down-regulation of MBP expression. IL-1β inhibited the expression of p-ERK, ERK over-expression reversed the down-regulation of MBP expression compared with IL-1β group. CONCLUSION: IL-1β inhibits the differentiation of OPCs, which may be involved in ERK pathways, thus leading to axonal hypomyelination in the corpus callosum of septic neonatal rats.     

广西野生鳞盖红菇原种培养研究

为获得野生鳞盖红菇原种,利用通过组织分离获得的母种,根据母种分离培养经验并利用各种食用菌原种培养料搭配成多种配方培养红菇原种。结果表明:花生饼对原种培养有较大促进作用;麦子较适合作为培养原种主原料;配方棉籽壳15%、麦麸10%、麦子57%、玉米粉8%、花生饼8%、蔗糖0.8%、石膏1%、硫酸镁0.2%能长出较合格原种。     

Numerical Simulation of Elastoplastic Multi-scales Model for Magnesium Alloy Under Tensile Load

In order to investigate the macroscopical mechanical performance of magnesium alloy material containing pore and the mechanism during distorting destructing, an accurate finite element model of magnesium alloy using the multiscales method was developed, and the macroscopic properties of material under the macroscopic tensile load using the developed constitutive model of multi-scales and finite element method was obtained. By analyzing the distortion results of meso-model through the multi-scales method, with macroscopical load increasing, the difference demonstrated on the macroscopic is very small, however, with the increase of the load step, the phenomenon that the ability of plasticity deformation of the meso-models which includes the cuboid pore decreased gradually remarkable and the phenomenon of stress centralized is more severity than the meso-models including cylindrical pore could be obviously observed during the process of deformation on the meso-models.     

Improvement Algorithm of Generation Cost Based on Reliability

This paper discusses the influenced manner of reliability on bidding, and establishes the direct functional relationship among reliability, generation cost and electricity price in different risk of power generation. In this function model, the generation cost and the price could reflect the current reliability of equipment in a dynamic way. The generation risk is also considered in competitive bidding. The bidding strategy based on this model helps the power plant acquire the optimal benefit.     

红地球葡萄日灼病与反光膜套袋初报

在2002年5～7月,用塑料反光膜作外袋、白色葡萄套袋为内袋制成双层反光葡萄套袋,进行红地球葡萄防日灼病的试验研究。结果表明:反光葡萄套袋内温度低于气温0～2℃,当气温为38℃时,该套袋内红地球幼果均未发现日灼现象,防日灼效果显著。     

Quantifiable Technique for Product Definition

近年来，我国水果种植业发展十分迅速，总产量跃居世界第一位。但是，一种奇怪的现象一直困扰着水果市场，一方面农民卖果难；另一方面水果出口数量发展缓慢，国内市场终端的水果质量也不尽人意。除了某些水果品种过时、栽培技术有待改进外，另一个主要原因就是水果物流过程中存在着诸多问题。提高我国水果在国际市场的竞争能力，改善我国水果市场终端的商品品质，减少水果采后物流中的损耗是摆在水果研究人员面前亟待解决的问题。本文将对几种有代表性水果的物流现状进行概述，并就目前水果物流中存在的问题进行分析。１红富士苹果１．１主…