宁波市竹资源培育、利用及发展策略

对宁波市竹林资源情况、竹林培育及竹资源利用的现状进行了分析,阐明了宁波市竹产业开发过程中存在的一些问题,并以竹资源可持续发展为基础,就这些问题提出了相应的策略:实施并加快竹林基地建设;创新培育模式;发展、创新竹林培育与加工技术;完善政策。     

硝酸还原酶的研究——Ⅴ.棉花硝酸还原酶活力与硝态氮含量的关系

棉花中的酚类物质对硝酸还原酶(NR)活力的体外测定有强烈抑制作用。用含半胱氨酸(5mM)和乙二胺四乙酸二钠(5mM)的磷酸缓冲液提取测不出酶活。如在磷酸缓冲液中加入酚类物质的抑制剂—聚乙烯吡咯烷酮(PVP),酶活力则显著增高。运用此法,本文在盆栽的情况下研究了棉花 NR 活力和硝态氮含量的关系。结果表明在不同棉花品种中、不     

中国主要纸产品国际竞争力综合评价

文中在分析中国主要纸产品出口贸易现状的基础上,选取国际市场占有率、贸易竞争力指数和显示性比较优势指数测算中国主要纸产品2010-2019年的国际竞争力指数,并采用基于主成分分析的定权方法对纸产品国际市场竞争力进行综合评价。结果表明:主要纸产品国际竞争力较强的芬兰、瑞典和德国位居综合评价前3位,中国主要纸产品综合竞争力经过近10年发展已处于中等偏上水平,仍有改进和提升空间。基于上述研究成果,提出推进人工林速生材高效利用、促进科技创新能力提升和探索"出口转内销"等政策建议。     

园林保水剂在温室西红柿上的应用效果初报

对保水剂采用温室挂置和土壤施入两种使用方式在温室西红柿上进行应用效果试验。结果表明：园林保水剂对温室温度、湿度有较好调节作用,特别是在12：00和16：00时间,温度和相对湿度比对照要高,保水剂能提高株高、茎粗和产量,降低病株率效果均不错。两种方式中,土壤施入方式效果更好。     

草酸青霉菌产酶条件优化及其秸秆腐解能力

目前还田的玉米秸秆存在腐解难的特点,如何加速玉米秸秆腐解成为国内外研究的热点。该试验采用纤维素-刚果红染色法从河北山前平原小麦-玉米轮作且长期秸秆还田的土壤中分离筛选到一株纤维素酶活性高的真菌。将测得的ITS基因序列与NCBI数据库上进行同源性比对,综合形态特征和ITS基因序列同源性分析,该菌株鉴定为草酸青霉(Penicillium oxalicum)。该文对其产酶条件和玉米秸秆分解能力进行了进一步研究。结果表明,在羧甲基纤维素钠(CMC-Na)液体培养基和玉米秸秆液体培养基上30℃培养72 h,内切葡聚糖酶(CMCase酶)和滤纸酶(FPase酶)活性分别达672.8、282.9和774.6、618.3 U;以玉米秸秆为底物其产天然纤维素总酶活性可达到376.1 U,说明该菌具有较强的玉米秸秆分解能力。该菌最佳产酶条件为:0.3%的牛肉膏蛋白胨为氮源,接种量为5%,培养温度为28~35℃,pH值=4~7,培养48~96 h;最优发酵条件为固液比为1∶10,培养时间为48 h,培养温度为30℃,pH值为6.5。该菌株对玉米秸秆腐解能力的研究表明,在秸秆揺瓶试验中,培养10 d后,秸秆腐解率达87.3%,为对照的1.90倍;在土壤培养试验中,培养45 d时,腐解率达83.5%,是对照的1.62倍;在玉米秸秆还田的小麦盆栽试验中,培养65 d时,秸秆腐解率达70.8%,高于对照15.1%。因此,从原位秸秆还田土壤中筛选出来的草酸青霉对玉米有较强的腐解能力。     

Establishment of an in vitro method for evaluating whitefly resistance in tomato

An accurate and simple evaluation method is crucial for identifying whitefly resistance in tomato breeding. We developed an in vitro method for evaluating resistance of tomato leaves and tested this on wild and cultivated tomato varieties. We found that young leaves observed for whitefly oviposition after 8 hours provided appropriate comparative conditions. This method effectively distinguished resistance among tomato cultivars and wild species and also demonstrated significant difference in oviposition rates among leaf positions on susceptible cultivars. The in vitro test was as precise as in vivo test using intact plants and had advantages over in vivo test, and can be used for evaluating resistance in large populations.     

动物、动物产品药物残留及应对措施

动物性食品中的药物残留量虽然很低,但对人体健康的潜在危害却甚为严重,对畜牧业生产影响巨大,而且影响深远,是国内外普遍关注的     

鸡肠炎沙门氏菌外膜蛋白F的原核表达及免疫原性分析

根据GenBank中收录的鸡肠炎沙门氏菌OmpF基因序列设计1对引物,以鸡肠炎沙门氏菌辽宁分离株基因组为模板,扩增鸡肠炎沙门氏菌OmpF基因,用IEDB Analysis Resource在线预测分析,该基因可能存在15个线性B细胞抗原表位;同时将该基因克隆至原核表达载体PGEX-4T-1中,构建重组表达质粒PGEX-4T-1-OmpF,转入大肠杆菌BL21中,获得OmpF重组蛋白。Western-blot检测结果表明,该重组蛋白的分子质量约为66 kD(含标签蛋白),能被鸡沙门氏菌阳性血清识别,初步证实该蛋白具有免疫原性,为鸡肠炎沙门氏菌亚单位疫苗的进一步研究和开发奠定了理论基础。     

猪繁殖与呼吸综合征病毒实验感染SPF猪外周血单核细胞和肺泡巨噬细胞早期凋亡细胞的观察

采用Ａｎｎｅｘｉｎ Ｖ－ＦＩＴＣ／ＰＩ双染色法,用流式细胞仪检测了猪繁殖与呼吸综合征病毒（ＰＲＲＳＶ）实验感染ＳＰＦ猪不同时期外周血单核细胞和肺泡巨噬细胞感染Ａｎｎｅｘｉｎ Ｖ－ＦＩＴＣ＾＋／ＰＩ＾－细胞群（早期凋亡细胞群）。结果显示,ＰＲＲＳＶ感染猪外周血单核细胞和肺泡巨噬细胞Ａｎｎｅｘｉｎ Ｖ－ＦＩＴＣ＾＋／ＰＩ＾－细胞群的表达率均明显高于正常对照猪,感染后２４ｈ表达率达最高值。     

Age-related changes in the anatomical characteristics of Peyer's patches in small intestine of Bactrian camels (Camelus bactrianus)

The distribution, size, and appearance of Peyer's patches vary according to species. In order to determine the anatomical characteristics of Peyer's patches in small intestine of Bactrian camel, and age-related changes in the number of Peyer's patches, 40 Bactrian camels of the following four age groups were studied: young (0.5–2 years), pubertal (3–5 years), middle-aged (6–16 years), and old (17–20 years). The exact number of Peyer's patches was recorded, and the appearance of Peyer's patches was described in detail. The results indicated that Peyer's patches of Bactrian camels not only have a particular anatomical location and distinct appearance but also change with age. They were distributed in the whole small intestine and there were four distinct types of Peyer's patches: nodular, faviform, cup-shaped, and cystic form Peyer's patches. However, the nodular and cystic form Peyer’s patches are specific to Bactrian camel, which have not been found in other animals including Dromedary camel. In addition, the distribution density of Peyer's patches in ileum was the maximum, then was jejunum and duodenum. Further statistical analysis showed that the number of Peyer's patches was altered with age. The number peaked in 5-year-old camels and declined subsequently with age. However, there was little change in the size of Peyer's patches in different age groups; no age-related macroscopic variations in the shape or size of the Peyer's patches were found. Results obtained from this study provide the basic information to further study on the gastrointestinal mucosal immunity of Bactrian camel.