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51.
Y Ukishima M Kino H Kubota S Wada S Okada 《Journal of the Association of Official Analytical Chemists》1991,74(6):943-950
Thin-layer isoelectric focusing was applied to the identification of whale (Cetacea) species by using water-soluble sarcoplasmic proteins of skeletal muscles. Twenty-eight samples consisting of 4 species (10 samples) of baleen whales (Mysticeti) and 8 species (18 samples) of toothed whales (Odontoceti) were analyzed. Each sample (approximately 1 g) was electrophoresed with Ampholine PAGplate, pH 3.5-9.5. The electrophoretic profiles were species-specific on the 4 toothed whale species that did not have a marked intra-species difference, and all 4 baleen whale species. However, the profiles were not specific on the 4 other dolphin species, even though they were discriminable from the other 4 toothed whale species. Numerical values of pIs and relative peak heights were obtained by densitometric analysis of the isoelectro-focused protein bands. The bands were also species-specific for the 8 toothed whale species mentioned. The values may be applicable to species identification without the need for a standard sample, which may not be readily obtainable. Experiments on test samples of minke and sel whales showed that bloodletting with ice water made the densities of isoelectro-focused bands thinner, although species identification was still possible by using the inside part of muscles. Heat treatment at below 60 degrees C for 10 min caused little denaturation; at higher temperatures the protein bands were diminished in a temperature-dependent fashion. Therefore, the present isoelectric focusing analysis should be applicable to small samples of whale meat, excluding several species of dolphins. 相似文献
52.
Abrahamian Peter Sobh Hana Seblani Rewa Abou-Jawdah Yusuf 《European journal of plant pathology / European Foundation for Plant Pathology》2015,142(3):521-530
European Journal of Plant Pathology - In the past two decades, viruses in the genera Crinivirus and Begomovirus, transmitted by whiteflies, have emerged as threatening diseases to cucurbit... 相似文献
53.
Abakari Godwin Luo Guozhi Shao Lina Abdullateef Yusuf Cobbina Samuel Jerry 《Aquaculture International》2021,29(3):1295-1315
Aquaculture International - This study investigated the effects of biochar application on the bacterial community structure in bioflocs and intestines of Oreochromis niloticus cultured in a biofloc... 相似文献
54.
55.
Comparisons among MRI signs,apparent diffusion coefficient,and fractional anisotropy in dogs with a solitary intracranial meningioma or histiocytic sarcoma 下载免费PDF全文
Masae Wada Daisuke Hasegawa Yuji Hamamoto Yoshihiko Yu Aki Fujiwara‐Igarashi Michio Fujita 《Veterinary radiology & ultrasound》2017,58(4):422-432
Although MRI has become widely used in small animal practice, little is known about the validity of advanced MRI techniques such as diffusion‐weighted imaging and diffusion tensor imaging. The aim of this retrospective analytical observational study was to investigate the characteristics of diffusion parameters, that is the apparent diffusion coefficient and fractional anisotropy, in dogs with a solitary intracranial meningioma or histiocytic sarcoma. Dogs were included based on the performance of diffusion MRI and histological confirmation. Statistical analyses were performed to compare apparent diffusion coefficient and fractional anisotropy for the two types of tumor in the intra‐ and peritumoral regions. Eleven cases with meningioma and six with histiocytic sarcoma satisfied the inclusion criteria. Significant differences in apparent diffusion coefficient value (× 10?3 mm2/s) between meningioma vs. histiocytic sarcoma were recognized in intratumoral small (1.07 vs. 0.76) and large (1.04 vs. 0.77) regions of interest, in the peritumoral margin (0.93 vs. 1.08), and in the T2 high region (1.21 vs. 1.41). Significant differences in fractional anisotropy values were found in the peritumoral margin (0.29 vs. 0.24) and the T2 high region (0.24 vs. 0.17). The current study identified differences in measurements of apparent diffusion coefficient and fractional anisotropy for meningioma and histiocytic sarcoma in a small sample of dogs. In addition, we observed that all cases of intracranial histiocytic sarcoma showed leptomeningeal enhancement and/or mass formation invading into the sulci in the contrast study. Future studies are needed to determine the sensitivity of these imaging characteristics for differentiating between these tumor types. 相似文献
56.
Kei Nakagawa Shin-Ichiro Wada Ryoma Kitamura Ronny Berndtsson 《Communications in Soil Science and Plant Analysis》2017,48(21):2594-2600
Groundwater pollution by nitrate from agricultural fields is a worldwide problem. To improve the understanding of nitrate transport processes through volcanic ash soils overlying groundwater aquifers, salt sorption experiments were performed. Salt sorption is the process that involves the simultaneous use of cation and anion adsorption mechanisms without changing the pH. Prepared six different concentrations of potassium chloride (KCl) solutions were mixed with soil samples. Anion and cation contents of the liquid and solid phases were determined. Experiments were performed in triplicate of six kinds of KCl solutions for three types of soils. Thus, total 54 of samples were analyzed. Relationships between ionic strength and sorption capacity increments of cations and anions were determined. The results show that if ionic strength of the infiltrating solution to the volcanic ash soil is known, the ECEC (effective cation exchange capacity) and EAEC (effective anion exchange capacity) can be reliably estimated. The resulting prediction equations can be included to improve reactive transport simulation models. Consequently, it will be possible to better understand solute transport with changing sorption capacity for the solid soil phase. 相似文献
57.
The residual effect of organic and mineral fertilizers on selected chemical properties of an Alfisol in the long term soil fertility trial established in 1950 at Samaru, Nigerian savanna was assessed. The trial was left fallow for fourteen years due to lack of funds. Topsoil was collected from plots that received three levels of cow dung (D), nitrogen (N) and phosphorus (P) in all possible combinations. The soils were analyzed for selected chemical properties. Except for significant effect of applied P on available P, sole application of cow dung, N or P had no significant effect on all the measured soil properties. The interaction of D and P significantly affected the mean values of exchangeable Ca, Mg, K and ECEC in the range of 1.12-1.96, 0.62-1.11, 0.37-0.64 and 2.82-4.11 cmol/kg respectively. The most important results were the ability of the plot that received neither D nor P to significantly increase these parameters than the plots that received only one of the treatments. The results show that the fallow period has changed the effects of application of organic and mineral fertilizers on the soil chemical properties under continuous cultivation by modifying them towards those of a native savanna Alfisol. 相似文献
58.
Hidenori Wada Supamard Panichsakpatana Makoto Kimura Yasuo Takai 《Soil Science and Plant Nutrition》2013,59(3):449-452
Abstract Recently there has been developments in the measurement of N2 fixation due mainly to the C2H2 reduction method (1). This method, however, has several disadvantages, especially for submerged soil, and the estimated amount of fixed N2 on the basis of the C2H2 reduction activity is not very reliable. The tracer 15N2 technique which gives a reliable estimation of the fixed N2 is too expensive for common use. Development of an alternative method suitable for submerged soil would therefore be desirable. The present authors expected that the measurement of the ratio N2/Ar in the soil solution might provide advantages for the estimation of the fixed N2 in submerged soil. 相似文献
59.
Koji Wada Yasutaka Tsumori Yoshikazu Nitawaki Kazuhiko Egashira 《Soil Science and Plant Nutrition》2013,59(3):313-322
Flocculation tests and permeability measurements were carried out for silt and clay fractions separated from surface and subsurface horizons of four paddy soils. There was not much difference in the flocculating power between A1(OH)2.2C10.8 and CaSO4. Their flocculation value, 0.3 to 3 mM, was attained by adding the reagents at the rate of 0.24 to 2.4 mmol/100 g of the silt and clay. The flocculation with A1(OH)2.2C10.8 or CaSO4 did not affect the sediment volume of the silt and clay. This sediment volume was smaller for the surface than for the subsurface horizons of each soil, suggesting that soil organic matter and repetition of wetting and drying contribute to the stable aggregation of the silt and clay in the surface horizon. The permeability was higher for the silt and clay fractions separated from the surface horizon than that from the subsurface horizon, and the addition of 2.5 to 4.2 mmol of A1(OH)2.2C10.8 or CaSO4/100g of the silt and clay increased its permeability by 1.5 to 4 times. The effect of A1(OH)2.2C10.8 was more lasting than that of CaSO4 and was recognized even after 200 ml of water passed through 1 g of the silt and clay. The effect of the flocculating reagent on the permeability was more or less enhanced by air-drying the treated silt and clay. 相似文献
60.
Koji Wada 《Soil Science and Plant Nutrition》2013,59(3):137-144
It is generally accepted that broken bonds are the major cause of cation exchange in kaolin minerals, although the studies of a “fireclay” type of kaolin mineral (8) and hydrated halloysite (12, 15) seem to indicate that lattice substitutions, such as that found for 2 : 1 type of clay minerals, account for the fairly large parts of the cation-exchange capacities of some kaolin clays, particularly with poor crystallinity. 相似文献