Major flavonoids in grape seeds and skins: antioxidant capacity of catechin, epicatechin, and gallic acid

Grape seeds and skins are good sources of phytochemicals such as gallic acid, catechin, and epicatechin and are suitable raw materials for the production of antioxidative dietary supplements. The differences in levels of the major monomeric flavanols and phenolic acids in seeds and skins from grapes of Vitis vinifera varieties Merlot and Chardonnay and in seeds from grapes of Vitis rotundifolia variety Muscadine were determined, and the antioxidant activities of these components were assessed. The contribution of the major monomeric flavonols and phenolic acid to the total antioxidant capacity of grape seeds and skins was also determined. Gallic acid, monomeric catechin, and epicatechin concentrations were 99, 12, and 96 mg/100 g of dry matter (dm) in Muscadine seeds, 15, 358, and 421 mg/100 g of dm in Chardonnay seeds, and 10, 127, and 115 mg/100 g of dm in Merlot seeds, respectively. Concentrations of these three compounds were lower in winery byproduct grape skins than in seeds. These three major phenolic constituents of grape seeds contributed <26% to the antioxidant capacity measured as ORAC on the basis of the corrected concentrations of gallic acid, catechin, and epicatechin in grape byproducts. Peroxyl radical scavenging activities of phenolics present in grape seeds or skins in decreasing order were resveratrol > catechin > epicatechin = gallocatechin > gallic acid = ellagic acid. The results indicated that dimeric, trimeric, oligomeric, or polymeric procyanidins account for most of the superior antioxidant capacity of grape seeds.     

Neural-Intelligent Water Drops algorithm to select relevant textural features for developing precision irrigation system using machine vision

Sunagoke moss Rachomitrium japonicum is a good potential for greening material. One of the primary determinants of Sunagoke moss growth is water availability. Too much or too little water can cause water stress in plants. Water stress in plants can be detected by imaging. This study is part of on-going research aimed at developing machine vision-based precision irrigation system in a closed bio-production system for cultured Sunagoke moss. The objective of this study is to propose nature-inspired feature selection techniques to find the most significant set of Textural Features (TFs) suitable for predicting water content of cultured Sunagoke moss. The proposed Feature Selection (FS) methods include Neural-Intelligent Water Drops (N-IWD), Neural-Simulated Annealing (N-SA), Neural-Genetic Algorithms (N-GAs) and Neural-Discrete Particle Swarm Optimization (N-DPSO). TFs consist of 120 features extracted from grey, RGB, HSV, HSL and L^∗a^∗b^∗ colour spaces using ten Haralick’s textural equations. Back-Propagation Neural Network (BPNN) model performance was tested successfully to describe the relationship between water content of Sunagoke moss and TFs. Red Colour Co-occurrence Matrix (CCM) TFs, L^∗ CCM TFs, grey CCM TFs, value_(HSV) CCM TFs, green CCM TFs and lightness_(HSL) CCM TFs are recommended as individual feature-subset to be used for predicting water content of Sunagoke moss using Artificial Neural Networks. However, FS methods improve the prediction performance. The results show a significant difference between model using FS and models using individual feature-subsets or without FS. Comparative analysis shows the superiority of Neural-Intelligent Water Drops (N-IWD) compared to the other FS methods, since it achieve better prediction performance. The best N-IWD’s fitness function converged with the lowest validation-set Root Mean Square Error (RMSE) of 1.07 × 10⁻² when using 36 TFs.     

Tetraselmis chuii biomass as a potential feed additive to improve survival and oxidative stress status of Pacific white-leg shrimp Litopenaeus vannamei postlarvae

International Aquatic Research - Shrimp is an important traded fishery commodity. When subjected to stress, shrimp usually suffers from oxidative stress, which leads to cell injury, senescence, and...     

Response of selected water chemical quality parameters to slight thinning in a mature oak–beech forest ecosystem under sub-humid climate conditions

The objective of this study was to determine the effect of 18% thinning on streamflow nutrient flux from a mature oak–beech forest ecosystem by paired watershed approach. Two experimental watersheds including control (W-I) and treatment (W-IV) watersheds were used in the study. The experimental watersheds were monitored about 6 years from 2006 to 2011 for the calibration period and 4 years from 2012 to 2015 for the treatment period. The forest in the treatment watershed was thinned between October and December in 2011, and the forest in the control watershed was left untreated. Water grab samples were collected from the streams in the watersheds on weekly basis during both the calibration and treatment periods and analyzed for calcium (Ca²⁺), magnesium (Mg²⁺), Kjeldahl nitrogen (KN), sodium (Na⁺), potassium (K⁺), iron (Fe³⁺), aluminum (Al³⁺), ammonium nitrogen (NH₄ ⁺-N), and sulfate (SO₄ ²⁻). The simple linear regression equations were developed between mean monthly nutrient fluxes of two watersheds in the calibration period with significantly high correlation coefficients, and they were used to estimate nutrient fluxes from the treatment watershed during the treatment period as if thinning had not been applied. The changes in the monthly nutrient fluxes were estimated as the differences between measured and values calculated with the linear regression equations. Results showed that removal of 18% standing timber volume did not significantly change nutrient exports except for KN and Na⁺ from the treatment watershed.

     

Orthogonal cutting mechanics of maple: modeling a solid wood-cutting process

The experimental results of orthogonal cutting of maple and the modeling of the cutting mechanics are presented. The tool cutting forces were measured for different feed rates. A set of equations relating the tangential and feed forces to the tool edge width and feed rate (chip thickness) to calculate the chip and edge cutting force coefficients was developed. Then the chip force and edge force coefficients were calculated from experimentally obtained cutting forces and were plotted in a polar-coordinate system with respect to the fiber orientation of the maple disk. The polar-coordinate presentation of the cutting force results and the calculated cutting force coefficients provides an excellent visual appreciation of the relation between the cutting forces and the wood fiber orientation. Chips were also collected from various sectors of the wood disk. This analysis further identified the effects of fiber orientation and cutting forces on the types of chip formed and hence the cutting mechanics involved. By applying the calculated cutting coefficients for each tool orientation (in respect to the grain) it is possible to predict the feed and tangential forces for any feed rates. There is good agreement between the predicted and measured cutting forces.     

尼日利亚稻田养鱼加灯光诱虫应用效果分析

在尼日利亚科基州进行不同沟凼的稻田养鱼加灯光诱虫应用研究,其沟凼模式分围沟和十字沟,灯光以煤油灯代替电灯,鱼稻品种均为当地常见品种。结果表明,开挖占稻田面积10％～15％不同类型的鱼沟或鱼坑,其投入成本相同,鱼稻产量相近,均比单纯稻田收入增加3．5～4．5倍。示范成果还进一步验证了以往在尼日利亚进行的灯光诱虫试验结论,即在稻田养鱼中增加灯光诱虫,能够将有害昆虫变为鱼的全价饲料,减少稻田的病虫害,鱼、稻分别增产14％和9．5％,达到经济效益和生态效益双丰收。     

The distribution of important sero-complexes of flaviviruses in Malaysia

Tropical Animal Health and Production - Flaviviruses (FVs) are arthropod-borne viruses of medical and veterinary importance. Numerous species of FVs have been isolated from various host; mainly...     

Genome analysis of methicillin‐resistant Staphylococcus aureus isolated from pigs: Detection of the clonal lineage ST398 in Cameroon and South Africa

Food animals are considered reservoirs of methicillin‐resistant Staphylococcus aureus (MRSA) and are implicated in their zoonotic transmission in the farm‐to‐plate continuum. LA‐MRSA has been reported as a zoonotic agent that has the potential to spread to humans and may cause infections in at‐risk groups. In this study, whole genome sequencing was used to describe the genetic environment (resistance mechanisms, virulence factors and mobile genetic elements) and investigate the genetic lineages of MRSA isolates from pigs in Cameroonian and South African abattoirs. During March–October 2016, 288 nasal and rectal pooled samples from 432 pigs as well as nasal and hand swabs from 82 humans were collected. Genomic DNA was sequenced using an Illumina MiSeq platform. Generated reads were de novo‐assembled using the Qiagen CLC Genomics Workbench and SPAdes. The assembled contigs were annotated, and antibiotic resistance genes, virulence factors, plasmids, SCCmec and phage elements were identified with ResFinder, Virulence Finder, PlasmidFinder, SCCmec Finder and PHAST, respectively. Core genome single nucleotide analysis was undertaken to assess clonal relatedness among isolates. A lower MRSA prevalence was observed in pigs in Cameroon (n = 1/13; 0.07%) compared with South Africa (n = 4/22; 18.18%), and none of the workers were colonized by MRSA. Genome analysis identified various antibiotic resistance genes along with six virulence factors in all isolates. All MRSA isolates belonged to the clonal lineage ST398 (spa‐type t011) and harboured the type Vc SCCmec and several plasmids. Our study shows that the livestock‐associated MRSA clonal lineage ST398 is already present in both Cameroon and South Africa and is probably underestimated in the absence of molecular epidemiological studies. It reveals the serious food safety and public health threat associated with this animal strain and underscores the need for interventions to contain this resistant clone.     

Electricity and water desalination: Separate sites offer value

For the fuel type and water situation in the Middle East, the case is strong for the use of combined cycle technology for power generation and reverse osmosis for potable water production, where each are sited for their maximum economic benefit and interconnected by electric power transmission. Because of the fuel efficiency of Combined Cycle generation technology, its use of liquid/gas fuels and its low need for cooling water, it can be optimized for cost away from cities. Conversely, water desalination by reverse osmosis can be sited in optimal locations to take advantage of its modularity and to minimize water pipeline needs. Electric power transmission provides an inexpensive and flexible means to connect these two technologies. Together these technologies may offer an overall minimum cost approach, better than the combining of electric power and water desalination at one location, where power to water ratios must be fixed, independent of need, for optimum efficiency. The use of reverse osmosis with power generation has other, important ancillary benefits over using distillation and power combinations. These advantages include abatement of environmental pollution, delivery of potable water at reasonable drinking temperatures, lower total energy consumption, more efficient land use and less demanding operator skills.     

Can diphenhydramine prevent organophosphate-induced acute pancreatitis? An experimental study in rats

Acute pancreatitis (AP) is a well known complication of organophosphate (OP) poisoning and the true incidence is unknown; but, may be more common than clinically suspected. Previous studies suggest that Diphenhydramine (DPH) may be useful as an alternative or adjunctive therapy in OP poisoning. The aim of this experimental study was to investigate whether DPH could prevent or diminish pancreatic damage caused by OP poisoning as defined by histologic findings, and serum interleukin 10 (IL-10) and tumor necrosis factor alpha (TNF-α) levels. Twenty-four Sprague- Dawley rats were divided into equal three groups. Group 1 did not receive any agent during the experiment. Group 2 received 0.8 g/kg fenthion subcutaneously followed by 3 ml/kg normal saline intramuscularly, 30 min later. Group 3 received 0.8 g/kg fenthion subcutaneously, followed by 30 mg/kg DPH intramuscularly, 30 min later. Twenty-four hours later, pancreatic tissues were excised and blood samples were taken. After blood samples were taken by cardiac puncture, the animals were sacrificed. Treatment with DPH significantly decreased the serum TNF-α and increased the serum IL-10 levels. DPH significantly reduced pancreatic damage, including edema, inflammation, vacuolization and necrosis, as determined by pathologic scoring. The present study show that DPH decreased the severity of OP induced AP in rats. This effects may be related to a decrease of TNF-α level and increase of IL-10 level.