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排序方式: 共有185条查询结果,搜索用时 15 毫秒
91.
92.
M Murakami C Matsuba Y Une Y Nomura H Fujitani 《Journal of veterinary diagnostic investigation》2001,13(6):513-516
Previously, a nested polymerase chain reaction (PCR) was employed with consensus degenerate primers targeting highly conserved motifs within herpesviral DNA polymerase genes to detect a newly described tortoise herpesvirus. However, nucleotide sequence information obtained from the final amplified fragment was restricted to a small region of 181 bp. In the present study, additional sequences flanking this segment were determined from a PCR product successfully amplified using a set of known degenerate primers, which covered a 692-bp region within the tortoise herpesviral DNA polymerase gene. Polymerase chain reaction primers for specific amplification of the tortoise herpesviral DNA were designed on the basis of these nucleotide sequences and successfully amplified tortoise herpesviral DNA from the tissues of tortoises that were well characterized histopathologically with herpesviral infection. The lower limit of detection was 1,000 herpesviral DNA equivalents in the presence of normal tortoise genomic DNA. Furthermore, a more sensitive and specific PCR technique for the identification of herpesviral infections in tortoises was developed employing a heminested form, which will enable the detection of latent infections or herpesvirus carriers in tortoises. 相似文献
93.
Yasuhiro Takahata Yumi KaiMasaru Tanaka Hiroki NakayamaMasaru Yoshinaga 《Scientia Horticulturae》2011
To investigate the effect of differences in anthocyanin composition on radical-scavenging activity in the purple-fleshed sweetpotato storage root, 124 sweetpotato clones with various compositions of anthocyanin were produced by crosses among superior sweetpotato genetic resources, and were analyzed for anthocyanin composition and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging activity (RSA). The highest content of anthocyanin among the clones was twofold higher than the content of cv. Ayamurasaki, which has been released for use in industrial pigment production in Japan. The range of variance in anthocyanin composition was very wide, as the ratio of peak areas of cyanidin-based anthocyanins to total anthocyanin peak areas ranged from 85.7% to 6.0%. The relationship between anthocyanin content and RSA was positively correlated. Correlation coefficients between RSA and the HPLC peak areas of cyanidin-based anthocyanins ranged from 0.606 to 0.687, whereas those of peonidin-based anthocyanins were 0.124–0.271. These results clearly demonstrated that cyanidin-based anthocyanins are closely related to DPPH RSA in sweetpotato storage roots. 相似文献
94.
Tokushi Komatsu Yumi Higashiyama Michiru Fukasawa Kazuo Katoh Akinori Oshibe 《Animal Science Journal》2014,85(3):271-276
In this study, we investigated the physiological changes in cattle during feeding and rumination. We collected blood samples every 5 min by using an automated blood sampling system and simultaneously recorded feeding, ruminating, and other behaviors using a video camera. Plasma non‐esterified fatty acid (NEFA) concentrations continuously decreased during feeding and decreased temporarily during rumination. Plasma glucose concentration continuously decreased during feeding and remained stable during rumination. During feeding and rumination, there were no characteristic increases and subsequent decreases in plasma insulin and growth hormone (GH) concentrations, although insulin concentrations were positively correlated with glucose concentration. NEFA concentrations were not correlated with GH and insulin concentrations. In terms of chewing behavior, feeding and rumination are similar; therefore, the changes in metabolites such as NEFA might have been the same. Combination of behavioral observations and application of an automated blood sampling system could contribute to new findings on behavioral and physiological changes regarding the temporary decrease in plasma NEFA concentration during rumination in ruminants. 相似文献
95.
Satomi Hashimoto Takuya Doi Yumi Wako Junko Sato Sou Wada Minoru Tsuchitani 《Journal of toxicologic pathology》2013,26(3):275-281
We have recently started using Wistar Hannover rats in Japan and are now collecting background data. We have been frequently observing corneal mineralization in Wistar Hannover rats of both the RccHanTM:WIST and Crl:WI (Han) strains. In this study, details of corneal mineralization in Wistar Hannover rats were histopathologically and ultrastructurally investigated. According to the results, Wistar Hannover rats had a much higher incidence of corneal mineralization compared with Sprague-Dawley rats. The incidence of corneal mineralization was higher in males than females. According to the histological examination, mineral deposits were positive for calcium by von Kossa’s method. Furthermore, in response to mineralization, keratocytes probably become active to play an important role against the mineralized substance. 相似文献
96.
97.
Takako Yazaki Yuki Hiradate Yumi Hoshino Kentaro Tanemura Eimei Sato 《Animal Science Journal》2013,84(5):395-402
We investigated the effect of oxidative stress induced by hydrogen peroxide (H2O2) on lipid peroxide (LPO) level and nuclear maturation in porcine oocytes cultured with or without cumulus cells. After 22 h of pre‐culture, oocytes with attached cumulus cells (COC group) or denuded oocytes (DO group) were cultured with H2O2, and intra‐oocyte H2O2 and LPO levels were quantitatively analyzed using immunofluorescence. This is the first report evaluating LPO levels in porcine oocytes. After H2O2 supplementation, the DO group showed severe accumulation of H2O2 and LPO in the oocytes. Similarly, while inhibition of progression of nuclear maturation was observed in both groups, the effect was more severe in the DO group. These results demonstrate that cumulus cells reduce the accumulation of H2O2 stress in oocytes. Furthermore, we attempted to reduce the oxidative stress by H2O2 with L‐carnitine, a H2O2 scavenger. L‐carnitine decreased H2O2 and LPO levels in the oocytes in both groups, and improvement in the progression of impaired nuclear maturation was observed. These effects were different by the presence of cumulus cells. Our results provide that L‐carnitine is useful for alleviating H2O2‐induced oxidative stress by reducing LPO levels and improving the progression of nuclear maturation. 相似文献
98.
Doi T Kotani Y Takahashi K Hashimoto S Yamada N Kokoshima H Tomonari Y Wako Y Tsuchitani M 《Journal of toxicologic pathology》2010,23(2):103-106
Spontaneous malignant mesothelioma was found in a 104-week-old male Crj:CD(SD) rat. The tumor was scattered on the surface of the lung, heart, mediastinal pleura and thoracic wall and metastasized to the alveolar septa. Histopathologically, small flattened or cuboidal tumor cells proliferated with stroma, formed almost normal papillary structures and reacted positively to colloidal iron stain and immunohistochemical staining for mesothelin. Round hyalinous stromata were pronounced, which is a characteristic feature, and the possible reason for this is as follows; at first, a small amount of collagen fibers was formed in the center of the clusters of several tumor cells, and then the cell clusters expanded like balloons with an increase in the collagen fibers. 相似文献
99.
100.
To demonstrate the psychophysical effect of edge-grain patterns on visual impressions received by human observers, printed images of the edge-grain patterns of 16 species were prepared as well-regulated visual stimuli, and impressions of the patterns on 32 subjects were evaluated using six categories. Image characteristics of the patterns were expressed numerically using contrast values that were derived by multi-resolution contrast analysis (MRCA) proposed by the authors, and relationships between the image characteristics and the visual impressions were examined. The difference in lightness between local areas with a certain size in the image was used as the contrast value, which could be used to detect the change in lightness due to the particular feature in the edge-grain pattern. While some impressions were almost independent of the contrast values, other impressions showed highly positive or negative linear relationships to the contrast values, derived at the optimal filter size in MRCA. Moreover, these optimal sizes were rarely affected by color information. The contrast value proposed in this paper would be a good numerical index that could be used not only to describe image characteristics of the edge-grain patterns but also to estimate some of their visual impressions. 相似文献