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101.
Major microbiota of lactic acid bacteria from Matsoni, a traditional Georgian fermented milk 总被引:1,自引:0,他引:1
Kenji UCHIDA Tadasu URASHIMA Nino CHANISHVILI Ikiti ARAI Hidemasa MOTOSHIMA 《Animal Science Journal》2007,78(1):85-91
A total of 26 samples of Matsoni were collected in Georgia. From these samples 80 strains of lactic acid cocci and 173 strains of lactobacilli were isolated. The number of lactic acid bacteria varied between 105 and 1010 colony forming unit (CFU)/mL. All the isolated lactic acid bacteria were thermophilic bacteria that could grow at 45°C. The predominant lactic acid bacteria were Streptococcus thermophilus and Lactobacillus delbrueckii spp. bulgaricus in 25 samples of Matsoni, while Lactobacillus helveticus was also a predominant species, together with the two previous species in one sample of Matsoni. We showed there was diversity in both S. thermophilus and L. delbrueckii spp. bulgaricus at the strain level by randomly amplified polymorphic DNA analysis. 相似文献
102.
103.
Watanabe Y Yang CH Tung KC Ooi HK 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(5):543-545
Several peripheral blood microfilaria concentration methods that use Acetone (Acetone test), 2% formalin (modified Knott method), 5% Tween 20 solution, distilled water, 1% or 0.1% SDS were compared for their efficacy in detecting Setaria digitata microfilaria in cattle. The Acetone test was found to be more efficacious than the modified Knott method or the 5% Tween 20 solution test for detecting the S. digitata microfilaria in bovine blood. However, besides the Acetone test, the modified Knott method was also found to be suitable for Dirofilaria immitis microfilaria detection in dogs. SDS and distilled water were found not to be effective as hemolytic agent for the disruption of the red blood cell of both the cattle and dogs. Thus, the Acetone test is recommended for the primary screening of microfilaremia of S. digitata in cattle. 相似文献
104.
Hayashi H Takata M Iwamaru Y Ushiki Y Kimura KM Tagawa Y Shinagawa M Yokoyama T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(5):515-520
Surveillance for bovine spongiform encephalopathy (BSE) in fallen stock in Japan is conducted with a commercial enzyme-linked immunosorbent assay (ELISA) for mass screening, with Western blotting (WB) and immunohistochemistry performed for confirmation of the ELISA. All tests are based on immunological detection of an abnormal isoform of the prion protein (PrP(Sc)) in brain tissues, which have sometimes deteriorated by the time samples from fallen stock reach a diagnostic laboratory. To evaluate BSE surveillance procedures for fallen stock, we examined PrP(Sc) detection from artificially deteriorated BSE-affected bovine brain tissues with a commercial ELISA kit and compared the results with those of WB. The optical density (OD) values of the ELISA decreased with advancing deterioration of the tissues, whereas no reduction in the signal for PrP(Sc) was observed in WB, even when performed after 4 days of incubation at 37 degrees C. The progressive decrease in the OD values in the ELISA appear to be caused by a partial loss of the N-terminal moiety of PrP(Sc) due to digestion by endogeneous and/or contaminated microbial enzymes, and by the presence of ELISA inhibitors that are generated in deteriorated tissues. These results suggest that WB is the most reliable test for fallen stock, especially for cattle brains within decaying carcasses. 相似文献
105.
Aruji Y Tamura K Sugita S Adachi Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(10):1283-1286
Microorganisms from 45 jungle crows (Corvus macrorhynchos) captured from July to December 2002 at Ueno Zoo, Tokyo were identified as Escherichia coli, Proteus mirabilis, Klebsiella pneumoniae, Klebsiella oxytoca, Enterobacter aerogenes, Enterobacter cloacae, Enterobacter agglomerans, Pseudomonas maltophila, Staphylococcus spp., Micrococcus spp., and Streptococcus spp. E. coli showed the highest rate of isolation (21.6%). In an in vitro susceptibility test for 29 isolates of E. coli to 14 antimicrobial agents, all the isolates were resistant to penicillin G, vancomycin, erythromycin, lincomycin, bicozamycin, sulfadimethoxine, and olaquindox. Several isolates of them were also resistant to tetracycline, oxytetracycline, streptomycin, chloramphenicol, and ampicillin. Twenty-nine isolates were divided into 19 serogroups and the most frequently identified serogroups were O8, O114 and O144, which showed the same multidrug-resistant patterns. 相似文献
106.
Koie H Shibuya H Sato T Sato A Nawa K Nawa Y Kitagawa M Sakai M Takahashi T Yamaya Y Yamato O Watari T Tokuriki M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(11):1453-1456
A castrated male border collie 23 months of age weighing 19.4 kg was referred to the Animal Medical Center of Nihon University with complaints of visual disturbance and behavioral abnormality, hyperacusis and morbid fear. The MRI examination revealed the slight dilated cerebral sulci and cerebellar fissures and left ventricular enlargement. This is the first report of MRI findings of canine neuronal ceroid lipofuscinosis. 相似文献
107.
Yuko Emoto Katsuhiko Yoshizawa Yuichi Kinoshita Michiko Yuki Takashi Yuri Airo Tsubura 《Journal of toxicologic pathology》2016,29(1):67-71
To evaluate the potential role of genetic background in the susceptibility to retinal degeneration induced by N-methyl-N-nitrosourea (MNU), female rats of the Sprague-Dawley (SD), Long-Evans (LE) and Copenhagen (CH) strains were administered 50 mg/kg MNU or saline at 7 weeks of age. Retina morphology and morphometric analysis of all rats was performed 7 days after MNU administration. Atrophy of both the peripheral and central outer retina occurred in all rat strains exposed to MNU. Decreased photoreceptor cell ratio and increased retinal damage ratio were observed. The severities of the retinal atrophy were similar among all three rat strains. In conclusion, MNU-induced photoreceptor degeneration developed consistently in all three strains regardless of the absence (SD rats) or presence (LE and CH rats) of melanin in the retina, suggesting that genetic and melanin factors did not affect photoreceptor cell death after MNU. 相似文献
108.
Yuko Ito Kohei Matsushita Takuma Tsuchiya Yukari Kohara Tsuyoshi Yoshikawa Makoto Sato Keisuke Kitaura Satoshi Matsumoto 《Journal of toxicologic pathology》2014,27(1):91-95
This report describes a spontaneous nephroblastoma with lung metastasis in a 10-week-old male Crl:CD(SD) rat. Macroscopically, a white mass in the kidney and two white masses in the lung were observed. Histopathologically, the renal mass was located in the cortex of a kidney, and it caused pressure on the surrounding renal parenchyma. Three components could be distinguished in the tumor: blastemal, epithelial (primitive glomerular/tubular structures) and mesenchymal (neoplastic connective tissues) elements. Immunohistochemically, the tumor cells were positive for Wilms tumor 1 protein (WT1) and vimentin. Metastasis was found in the lung. Thus, the case was diagnosed as a nephroblastoma with lung metastasis. 相似文献
109.
Kei IMURA James Kenn CHAMBERS Kazuyuki UCHIDA Shunsuke NOMURA Satoshi SUZUKI Hiroyuki NAKAYAMA Yasutsugu MIWA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(12):1667-1670
An 8-month-old common marmoset
(Callithrix jacchus) was presented with tic-like symptoms, and a
2-year-old pigmy marmoset (Callithrix pygmaea) was presented with dyspnea
and hypersalivation. Both monkeys died within a few days, and necropsies were performed.
Histopathological examinations revealed ulcerative stomatitis with epithelial cell
swelling and eosinophilic intranuclear inclusion bodies in the oral epithelium of both
cases. In the central and peripheral nervous systems, neuronal cell degeneration with
intranuclear inclusion bodies was observed. Immunohistochemical examination using
anti-herpes simplex virus type 1 antibody revealed virus antigens in both cases. Both
animals had been kept as pets with limited exposure to the ambient environment except via
their owners. Therefore, herpes simplex virus type-1 was probably acquired from close
contact with their owners. 相似文献