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71.
Tsuyoshi Ito Yoshitaka Katoh Yuko Shimada Aya Ohnuma-Koyama Naofumi Takahashi Maki Kuwahara Takanori Harada 《Journal of toxicologic pathology》2016,29(1):39-43
Extraskeletal osteosarcoma is extremely rare in mice. This case report demonstrates a spontaneous murine extraskeletal osteosarcoma that exhibited various histological growth patterns in an ICR mouse. At necropsy, the tumor mass was located in the abdominal wall and was 45 × 30 × 25 mm in size. Histopathologically, the tumor showed the following four growth patterns: a solid pattern of polygonal cells embedded in an osteoid eosinophilic matrix with calcification, an irregular sheet pattern of short spindle cells accompanying some eosinophilic multinucleated cells, a fascicular pattern of spindle cells and a cystic pattern lined by short spindle cells. Immunohistochemically, most of the tumor cells were positive for vimentin, proliferating cell nuclear antigen and osterix. The multinucleated cells mentioned above were desmin positive and were regarded as regenerative striated muscles but not tumor cells. Since no clear continuity with normal bone tissues was observed, the tumor was diagnosed as an “extraskeletal osteosarcoma.” 相似文献
72.
Takuro NAGAHARA Koichi OHNO Itsuma NAGAO Taisuke NAKAGAWA Yuko GOTO-KOSHINO Masaya TSUBOI James K. CHAMBERS Kazuyuki UCHIDA Hirotaka TOMIYASU Hajime TSUJIMOTO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(1):20
Intestinal lymphangiectasia (IL) is a common complication in dogs. Since nitric oxide (NO) is known to relax the lymphatic vessel, we evaluated inducible NO synthase (iNOS) expression using immunohistochemistry in 13 dogs with lymphoplasmacytic enteritis (LPE) with or without IL. The duodenal iNOS expressing cells were significantly increased in dogs with IL-negative or IL-positive LPE dogs (P=0.025, P=0.007) compared with control dogs. However, there was no significant difference in iNOS expression between IL-positive and IL-negative tissues. Based on these results, there is no clear evidence for the NO overproduction in the pathogenesis of IL in dogs with LPE. Factors other than NO could, thus, contribute to IL in dogs with LPE. 相似文献
73.
Miki YOSHIDA Kenjiro MIYOSHI Tomohiko TAJIMA Akihiko WADA Hiromi UEDA Takanori KOORIYAMA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(5):660
Irregular triangular cartilage or bone fragments are sometimes found in the fibrous triangle of the heart. Ossa cordis and/or cartilago cordis has been demonstrated in various terrestrial animal species. Regarding marine mammals, sperm whales lack heart bones, and there have been no studies on bones or cartilage in pinniped hearts. Therefore, we examined the ossa cordis and/or cartilago cordis of the Steller sea lion. Eleven Steller sea lion hearts were examined morphologically and histologically. Before dissection, some hearts were imaged by CT to confirm the presence of ossa cordis or cartilago cordis. As a result, ossa cordis-like fragments were confirmed in four adults and one pup. All of the fragments were found at the right fiber triangle, and one adult had ossified tissue, including adipose tissue in the bone marrow cavity. The ossa cordis probably support the aorta because they surround the aorta as in other terrestrial animals. Steller sea lions can dive to a few hundred meters, but they need to rest on land frequently. Hence, their ossa cordis help maintain heart function during the tachycardia that occurs upon repeated surfacing and movements on land after diving in water. 相似文献
74.
We investigated the effect of heat stress on Ca, P and Mg balance and bone turnover in lactating cows. In a 2 × 2 crossover design, four multiparous lactating Holstein cows were kept in a chamber and subjected to a constant moderate (18°C) ambient temperature (MT) or high (28°C) ambient temperature (HT). The cows were fed total mixed ration (Ca, 0.7%; P, 0.4%; Mg, 0.2%) ad libitum. The milk yield under HT (35.4 kg/day) tended to be lower (P < 0.10) than that under MT (43.2 kg/day). The concentrations of milk P (P < 0.05) and Mg (P < 0.01) were significantly lower under HT than MT. The Ca, P and Mg intake (P < 0.10); Ca (P < 0.10), P, and Mg (P < 0.05) secretion into milk; and Ca (P < 0.05), P (P < 0.01), and Mg (P < 0.05) absorption in the intestine were lower under HT than MT. The plasma osteocalcin, a marker of bone turnover, was significantly lower (P < 0.05) under HT than MT. Heat stress did not affect plasma C‐telopeptide of collagen type I, a bone resorption marker, and plasma parathyroid hormone concentration. 相似文献
75.
Kikumi OGIHARA Yuko NAYA Junichi KAMIE Shin HISAMATSU Michi KODAMA Yoshiharu ISHIKAWA Koichi KADOTA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(6):760
A cell line (PL38PB) was established from blood samples of a 6-month-old pig that was diagnosed with lymphoma with CD5 expression. Histopathological examination revealed neoplastic lesions in the spleen, liver and lymph nodes. Tumor cells were immunohistochemically positive for CD20 and immunoglobulin heavy chains (μ, γ and α). Membranous CD5 and cytoplasmic Immunoglobulin M (IgM), Immunoglobulin G (IgG) and Immunoglobulin A (IgA) were detected in PL38PB cells by flow cytometry. In addition, the cytoplasm of PL38PB cells were positive for IgM, IgG and IgA by immunofluorescent. However, no Ig secretion was detected in culture supernatant by Ouchterlony gel diffusion method. Results suggest that PL38PB cells express three Ig isotypes that are produced but not secreted. 相似文献
76.
Masanao Yokohira Yuko Nakano Nozomi Hashimoto Keiko Yamakawa Fumiko Ninomiya Sosuke Kishi Kousuke Saoo Katsumi Imaida 《Journal of toxicologic pathology》2012,25(4):257-263
In vivo, nicotine in cigarette smoke induces various effects not only on the
respiratory system but also the central and peripheral nerve systems, circulatory organs and
digestive organs, and there is a possibility of promotion of lung tumorigenesis. The present
experiment was conducted to examine histopathological changes caused by nicotine in the lung
with repeated intratracheal instillation (i.t.). Six-week-old male F344 rats were administered
nicotine by i.t. at doses of 0.05, 0.1 and 0.2 mg nicotine/rat every 3 weeks beginning at week
4, for up to a total of 9 times and were then sacrificed at week 30. The total number of
administrations, total dose of nicotine and effective number of rats were 9 times, 0.45 mg and
5 rats and 4 times, 0.20 mg and 5 rats for the 0.05 mg nicotine/rat group; 3 times, 0.30 mg and
5 rats and 4 times, 0.40 mg and 3 rats for the 0.1 mg group; and 3 times, 0.60 mg and 3 rats
for the 0.2 mg group, respectively. As a control group, 5 rats were administered 0.2 ml
saline/rat 9 times. Some rats administered 0.1 and 0.2 mg nicotine suffered convulsions just
after administration. Histopathologically, though proliferative changes were not observed,
neutrophil infiltration, edema and fibrosis in the lung were induced by nicotine. In
conclusion, repeated treatment of nicotine promoted neurologic symptoms in the acute phase, and
strong inflammation in the lungs in the chronic phase, even at a low dose. Toxicity of nicotine
is suggested to depend not on total dose of nicotine in the experiment but rather on repeated
injury with consecutive administration. 相似文献
77.
Taira O Watanugi I Hagiwara Y Takahashi M Arai S Sato H Maehara N 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2005,67(10):1059-1062
We cloned five new subtypes of cDNA encoding canine interferon-alpha (CaIFN-alpha) from a canine epithelial cell line. CaIFN-alphas were divided into two groups by amino acid sequences and a molecular phylogenic tree. Two subtypes of them were expressed in Escherichia coli, and IFN proteins were purified. Recombinant CaIFN-alphas were highly species-specific and showed antiviral activity against Vesicular stomatitis New Jersey virus and canine adenovirus-1 , but not against canine herpesvirus-1. 相似文献
78.
Kaewmanee S Watanabe G Keio M Yamamoto Y Yamamoto T Kishimoto M Nagaoka K Narushima E Katayanagi M Nakao R Sakurai Y Morikubo S Kaneko M Yoshihara M Yabe T Taya K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(3):379-383
This study was conducted to determine the correlation between reproductive hormones and musth in a male African elephant. Changes in circulating luteinizing hormone (LH), follicle stimulating hormone (FSH), testosterone and immunoreactive (ir-) inhibin and the degree of musth were evaluated for 4 years. LH increased 4 weeks before musth began. The highest concentrations of testosterone and ir-inhibin were observed from April to October. There were positive correlations among testosterone, ir-inhibin and musth behavior. These findings suggested that the surge-like LH in the pre-musth period might stimulate secretion of testosterone and ir-inhibin and thus initiate the musth behavior. This study also suggested that the high LH level before musth might be a useful biomarker for the beginning of the musth season. 相似文献
79.
Maho NAKAZAWA Hirotaka TOMIYASU Kanako SUZUKI Hajime ASADA Aki FUJIWARA-IGARASHI Yuko GOTO-KOSHINO Aki OHMI Koichi OHNO Michio FUJITA Hajime TSUJIMOTO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(3):456
Nasal lymphoma (NL) is the most common nasal tumor in cats, and radiotherapy, chemotherapy, or a combination of these treatments have been described as the treatment for this disease. However, the previous studies included various machines and protocols of radiotherapy. Therefore, we aimed to retrospectively compare the prognosis among cases treated with palliative hypofractionated radiotherapy, chemotherapy, and a combination of them with united machine and protocol of radiotherapy. When compared overall survival and progression free survival, there was no significant difference among these three groups. The data of this study suggested that similar efficacy could be achieved by palliative hypofractionated radiotherapy, chemotherapy, or a combination of them. 相似文献
80.
Teruo Nonomura Hiromi Tajima Yuko Kitagawa Naoko Sekiya Kayoko Shitomi Mami Tanaka Kazuhiko Maeda Yoshinori Matsuda Hideyoshi Toyoda 《Journal of General Plant Pathology》2003,69(1):45-48
Green fluorescent protein (GFP)-marked Fusarium oxysporum f. sp. melonis and nonmarked F. oxysporum f. sp. fragariae were stained with neutral red. The neutral red stained vacuoles of the fungi without disturbing GFP fluorescence in the cytoplasm.
GFP-marked fungi showed fluorescent hyphae with dark-stained vacuoles, whereas nonmarked fungi were detected as nonfluorescent
hyphae with dark-dotted vacuoles. Root colonization by these two fungi was monitored using this method. Microconidia attached
similarly to the root surface and elongated vegetative hyphae. Only the pathogenic fungi invaded, causing necrosis at the
inoculation site. Thus, the present method enabled us to track simultaneously the various formae speciales of F. oxysporum colonizing the root surface.
Received: March 25, 2002 / Accepted: September 27, 2002 相似文献