环境激励条件建筑结构的试验建模研究

通过环境激励模态识别技术对一座中高层新结构大楼环境激励试验建模研究。首先介绍了试验模型设计 ,并在现场测量整栋大楼在环境激励下的振动响应。然后采用新发展的频率空间域方法 ( FSDD)进行模态识别 ,分别在 0～ 4.5 Hz和 0～ 6.5 Hz频率范围识别出 9阶弯曲和扭转模态频率和振型。采用频率空间域方法识别了结构的阻尼特性 ,并得到满意的结果。所得试验模型已成功应用于 CFT大楼的有限元动态模型修正。所研发的试验建模技术可望在结构响应预报 ,健康监测和振动控制中发挥重要作用     

Induction of 12-chromosome plants from anther culture in a tuberousSolanum

Summary Haploid plants (2n=12) were produced by anther culture from the tuber-bearing wild speciesS. verrucosum.     

Expression and localization of tight junction-related proteins in adult rat pituitary stem/progenitor cell niches

Pituitary endocrine cells are supplied by Sox2-expressing stem/progenitor cells in the anterior lobe of the adult pituitary gland. These SOX2-positive cells are maintained in two types of microenvironments (niches): the marginal cell layer (MCL)-niche and the parenchymal-niche. Recently, we isolated dense SOX2-positive cell clusters from the parenchymal-niche by taking advantage of their resistance to protease treatment as parenchymal stem/progenitor cell (PS)-clusters. In the present study, by analyzing these isolated PS-clusters, we attempted to identify novel structural characteristics of pituitary stem/progenitor cell niches. Quantitative real-time PCR showed that tight junction-related genes were distinctly expressed in the isolated PS-clusters. Immunocytostaining showed that the tight junction molecules, ZO-1 and occludin, were localized in the apical membrane facing the pseudo-follicle-like structure of the isolated PS-clusters regardless of the expression of S100β, which distinguishes the sub-population of SOX2-positive cells. Furthermore, immunohistochemistry of the pituitary glands of adult rats clearly demonstrated that ZO-1 and occludin were densely present in the parenchymal-niche encircling the pseudo-follicle, while they were observed in the apical membrane in the MCL-niche facing the residual lumen. Collectively, these tight junction-related proteins might be involved in the architecture and maintenance of the plasticity of pituitary stem/progenitor cell niches.