排序方式: 共有83条查询结果,搜索用时 31 毫秒
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Saishu YOSHIDA Masashi HIGUCHI Hiroki UEHARU Naoto NISHIMURA Mitsuyoshi TSUDA Hideji YAKO Mo CHEN Hideo MITSUISHI Yoshiya SANO Takako KATO Yukio KATO 《The Journal of reproduction and development》2014,60(4):295-303
The pituitary is an important endocrine tissue of the vertebrate that produces and secretes many hormones. Accumulating data
suggest that several types of cells compose the pituitary, and there is growing interest in elucidating the origin of these cell
types and their roles in pituitary organogenesis. Therein, the histogenous cell line is an extremely valuable experimental tool
for investigating the function of derived tissue. In this study, we compared gene expression profiles by microarray analysis and
real-time PCR for murine pituitary tumor-derived non-hormone-producing cell lines TtT/GF, Tpit/F1 and Tpit/E. Several genes are
characteristically expressed in each cell line: Abcg2, Nestin, Prrx1,
Prrx2, CD34, Eng, Cspg4 (Ng2),
S100β and nNos in TtT/GF; Cxcl12, Raldh1,
Msx1 and Twist1 in Tpit/F1; and Cxadr, Sox9,
Cdh1, EpCAM and Krt8 in Tpit/E. Ultimately, we came to the following conclusions: TtT/GF cells
show the most differentiated state, and may have some properties of the pituitary vascular endothelial cell and/or pericyte.
Tpit/F1 cells show the epithelial and mesenchymal phenotypes with stemness still in a transiting state. Tpit/E cells have a
phenotype of epithelial cells and are the most immature cells in the progression of differentiation or in the initial
endothelial-mesenchymal transition (EMT). Thus, these three cell lines must be useful model cell lines for investigating pituitary
stem/progenitor cells as well as organogenesis. 相似文献
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KATO M 《Science (New York, N.Y.)》1958,127(3309):1291-1292
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ABSTRACT: The low salt extract from scallop striated adductor muscle contained extra proteins in addition to sarcoplasmic proteins and was turned into a gel upon standing or immediately by the addition of Ca2+ . The amount of extra protein, which was composed of a large amount of actin and a small amount of myosin, decreased with a decrease in adenosine triphosphate (ATP) content in the muscle during storage at 5°C. Actin was easily extracted from scallop myofibrils in low salt solution with ATP at 1 mM or above. Furthermore, ATP was required to induce the gelation of the low salt extract. A visual observation of the gelation of low salt extract is therefore a simple and easy method to inspect prerigor state of scallop adductor muscle. 相似文献
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Rieko OKAME Keiko NAKAHARA Yumiko KATO Makoto BANNAI Noboru MURAKAMI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(6):661-668
It has been suspected that in comparison with glucose or fatty acids, the levels of amino
acids may readily change with different forms of exercise. In the present study, we
measured the concentrations of amino acids, glucose, triglycerides, total protein and
total cholesterol in the blood and/or cerebrospinal fluid (CSF) of rats subjected to
forced running exercise on a treadmill, and voluntary running exercise using a wheel, with
a constant running distance of 440 m. Rats that performed no running and rats subjected to
immobilization stress were used as controls. We observed a few significant changes in the
levels of plasma glucose, triglycerides, total protein and total cholesterol in all
groups. Whereas, plasma amino acid levels were significantly changed by exercise and
stress, especially during the light period. The plasma levels of many amino acids were
specifically increased by forced running; some were decreased by immobilization stress.
Few amino acids showed similar changes in their levels as a result of voluntary running.
In addition, there was a significant difference in the degree of amino acid imbalance
between blood and CSF. These results provide the first information on changes in levels of
amino acids in plasma and CSF resulting from forced and voluntary exercises. 相似文献
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人工合成甘蓝型油菜的繁育特性与试管繁殖 总被引:5,自引:0,他引:5
通过甘蓝品种(2n=18,CC)与白菜品种(2n=20,AA)杂交并借助胚珠培养和染色体加倍,获得一系列人工甘蓝型油菜新品系。对18个人工合成甘蓝型油菜新品系的花粉育性、自交结实性、异交结实性等研究表明,人工油菜品系花期自交不结实或结实率很低。蕾期自交一朵花平均获3.6粒种子,平均结角率为19.7%。但是,人工甘蓝型油菜无论作父本还是作母本与天然甘蓝型油菜杂交的结实率都较高。以人工油菜作父本与天然油菜杂交。42个组合平均杂交一朵花获得10.3粒杂交种子;以人工油菜作母本与天然油菜杂交,15个组合平均杂交一朵花获得8.5粒杂交种子。分析结果表明。本试验的人工油菜品系具有很强的自交不亲和性,蕾期自交结实率也较低,但对异交结实影响不大,因此可以直接用于杂种生产。试验对自交结实特别困难的5个人工甘蓝型油菜新品系进行了试管繁殖,采取子叶、下胚轴和茎尖联合培养方式。22株无菌苗经过2个月快繁,获得260株苗。平均繁殖系数为12.4。不同基因型繁殖系数差异较大。变幅为3.3~21.0。 相似文献
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Yuko TOISHI Nobuo TSUNODA Masaaki TAGAMI Hiromitsu HASHIMOTO Fumiki KATO Tsukasa SUZUKI Kentaro NAGAOKA Gen Watanabe Shota TOKUYAMA Kiyoshi OKUDA Kazuyoshi TAYA 《Journal of Equine Science》2013,24(3):47-51
Evaluation of a new chemiluminescent enzyme immunoassay, the PATHFAST assay system
(PATHFAST), for measurement of circulating progesterone in mares was performed. Five mares
at the mid-luteal stage were administrated a single i.m. injection of prostaglandin F2α
analog (PGF2α; cloprostenol 250 μg/ml), and then blood samples were collected from the
jugular vein at 0, 15, 30 and 45 min, at one-hour intervals until 24 and at 48 hr via a
catheter in the jugular vein. To monitor the physiological changes in circulating
progesterone in mares after induced luteolysis, concentrations of progesterone in whole
blood and serum samples were measured by PATHFAST. In addition, concentrations of
progesterone in serum samples measured by PATHFAST were compared with those measured by
radioimmunoassay (RIA) and enzyme immunoassay (EIA). Using PATHFAST, the serum
concentrations of progesterone in mares correlated highly with those of whole blood
samples (r=0.9672, n=88). The serum concentrations of progesterone as measured by PATHFAST
correlated well with RIA (r=0.9654, n=88) and EIA (r=0.9323, n=112). An abrupt decline in
circulating progesterone in whole blood samples was observed within 2 hr (50%), followed
by a gradual decline until 48 hr later. The results for progesterone in whole blood
samples correlated highly with those in serum samples, and the declining pattern
paralleled that of the serum samples. These results demonstrated that PATHFAST is useful
in the equine clinic as an accurate diagnostic tool for rapid assay of progesterone within
26 min, using unextracted whole blood. 相似文献