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81.
Akinori Kiba Takako Ohgawara Kazuhiro Toyoda Miho Inoue-Ozaki Tadahiro Takeda Uppalapati Srinivasa Rao Toshiaki Kato Yuki Ichinose Tomonori Shiraishi 《Journal of General Plant Pathology》2006,72(4):228-237
In the plant cell wall of Pisum sativum seedlings, we found an NTPase (E.C. 3.6.1.5.) with ATP-hydrolyzing activity that was regulated by an elicitor and suppressors
of defense from pea pathogen Mycosphaerella pinodes. The ATPase-rich fraction was purified from pea cell walls by NaCl solubilization, ammonium sulfate precipitation, and chromatography
with an ATP-conjugated agarose column and an anion-exchange column. The specific activity of the final ATPase-rich fraction
increased 600-fold over that of the initial NaCl-solubilized fraction. The purified ATPase-rich fraction also had peroxidase
activity and generated superoxide, both of which were regulated by the M. pinodes elicitor and suppressor (supprescins). Active staining and Western blot analysis also showed that the ATPase was copurified
along with peroxidases. In this fraction, a biotinylated elicitor and the supprescins were bound primarily and specifically
to ca. 55-kDa protein (CWP-55) with an N-terminal amino acid sequence of QEEISSYAVVFDA. The cDNA clone of CWP-55 contained five ACR domains, which are conserved in
the apyrases (NTPases), and the protein is identical to a pea NTPase cDNA (GenBank accession AB071369). Based on these results, we discuss a role for the plant cell wall in recognizing exogenous
signal molecules. 相似文献
82.
OBJECTIVE: To report the clinical findings, surgical technique, and outcome after repair of urinary bladder rupture through a urethral incision in postpartum mares. STUDY DESIGN: Retrospective study. ANIMALS: Four Thoroughbred broodmares. METHODS: Medical records were reviewed for clinical signs, surgical technique, medical therapy, and outcome. The bladder was everted into the vagina through a urethral incision that included a sphincterotomy. The bladder defect was repaired with absorbable suture material in a single-layer, full thickness, simple, continuous pattern. The urethral incision was closed similarly. RESULTS: Depression, inappetence, signs of shock, dehydration, azotemia, and serum electrolyte abnormalities were consistent findings that increased temporally after bladder rupture. Each bladder defect was repaired successfully, and metabolic derangements were corrected with supportive medical therapy. All mares survived, conceived, and had more foals without further complications CONCLUSION AND CLINICAL RELEVANCE: Bladder rupture associated with parturition in mares can be repaired in a standing position by eversion of the bladder through a urethrotomy and urethral sphincterotomy. 相似文献
83.
Hiroyuki Takahara Kazuhiro Toyoda Gento Tsuji Yasuyuki Kubo Yoshishige Inagaki Yuki Ichinose Tomonori Shiraishi 《Journal of General Plant Pathology》2005,71(3):190-195
Mycosphaerella blight, caused by Mycosphaerella pinodes, is one of the major diseases of cultivated pea (Pisum sativum L.). To isolate the genes that are up- and down-regulated during spore germination, suppression subtraction hybridization (SSH) was performed between ungerminated and germinated spores. The 232 and 128 clones from forward and reverse libraries, respectively, were collected, sequenced, and analyzed with a BLASTX homology search. About 95% of the 32 selected clones were expressed during spore germination on a paper sheet and during infection of pea leaves. We discuss the applicability of the SSH libraries for analyzing M. pinodes genes involved in the early stage of infection. 相似文献
84.
Hiroyuki UGA Yuki O. KOBAYASHI Kyoji HAGIWARA Yohachiro HONDA Toshihiro OMURA Takahide SASAYA 《Journal of General Plant Pathology》2002,68(4):378-381
The causative virus (isolate No. 4) of gentian (Gentiana spp.) mosaic, which had been identified previously as Clover yellow vein virus (C1YVV) on the basis of host range and serological reactions, was re-identified as Bean yellow mosaic virus (BYMV) on the basis of the nucleotide sequences of the gene for the coat protein (CP) and the 3′-noncoding region, as well
as the predicted amino acid sequence of CP.
Received 16 April 2002/ Accepted in revised form 19 June 2002 相似文献
85.
Sakae Horisawa Masahide Sunagawa Yutaka Tamai Yuki Matsuoka Tohru Miura Minoru Terazawa 《Journal of Wood Science》1999,45(6):492-497
The physical properties of sawdust including porosity, water retention, and water drainage were analyzed to prove its suitability for use as an artificial soil in the automatic decomposer-extinguisher (GADE) machine. The physical and chemical properties of residual sawdust from the GADE machine were also analyzed, the mechanical abrasion of sawdust in the GADE machine was tested, and the morphology of this residue was observed through a scanning electron microscope to investigate changes of these properties in the medium of decomposing garbage. Sawdust, which showed a lower specific gravity and larger porosity than soil, is considered capable of supplying air to bacteria. It was found that sawdust became worn from the operation of the machine. The spaces of residual sawdust were still observed, but water drainage decreased. The portion of hollocellulose in residual sawdust decreased, although the extractives in it increased. Results indicated that the capacity of sawdust to function as an artificial soil in the GADE machine was decreased owing not only to the destruction of sawdust grain but also to the adherence of products from decomposition, such that sawdust needed to be replaced every few months.Part of this report was presented at the 43rd annual meeting of the Japan Wood Research Society, Morioka, April 1993 相似文献
86.
Jiang Tong Yuan Pengxiang Hirasaka Katsuya Hamada Yuki Hara Kenji Tachibana Katsuyasu Taniyama Shigeto 《Fisheries Science》2019,85(6):1099-1107
Fisheries Science - Blood is deposited in fish muscle during storage, and this deposition accelerates the deterioration of flesh quality. We have examined the effect of blood deposition on the... 相似文献
87.
88.
Trans geometric isomers of eicosapentaenoic acid (TEPA) have been found as minor components in human platelets. However, there
is little information on the mechanism of trans-isomerization of eicosapentaenoic acid (EPA) in vitro and in vivo. The effects of reactive radicals and heat on trans-isomerization of EPA were examined. Trans-isomerization occurred when EPA ethyl ester reacted with nitrogen dioxide radical (NO2) but not with 2,2′-azobis(2,4-dimethylvaleronitrile). TEPA was also produced from EPA ethyl ester heated at 200°C for 60
h. No TEPA, however, was detected in sardines Sardinops melanostictus after boiling, roasting, or microwave heating. These results suggest that EPA is trans-isomerized by NO2
in vivo while trans-isomerization of EPA does not occur during conventional cooking. 相似文献
89.
Terasawa N Saotome A Tachimura Y Mochizuki A Ono H Takenaka M Murata M 《Journal of agricultural and food chemistry》2007,55(10):4154-4159
Zuiki, a stalk of taro (Colocasia esculenta), is a traditional vegetable in Japan. Raw zuiki is often boiled and vinegared to eat. The surface color of zuiki is reddish. Here, we isolated a red pigment from zuiki and identified it as cyanidin 3-rutinoside using instrumental analyses. The color of zuiki disappeared by boiling, but the zuiki turned red again in an acetic acid solution. It seems that the cyanidin 3-rutinoside that exists on the surface of zuiki elutes in boiling water and then, the pigment that seeps out from the inside of the zuiki is exposed to an acid solution, and its surface turns red again. The radical scavenging activity of purified zuiki anthocyanin was 114 mg equivalent to BHT/g. About half of the anthocyanin in fresh zuiki was washed out by boiling, and the radical scavenging activity of zuiki was definitely reduced. 相似文献
90.
Sato Y Tsubono Y Nakaya N Ogawa K Kurashima K Kuriyama S Hozawa A Nishino Y Shibuya D Tsuji I 《Public health nutrition》2005,8(3):309-314
OBJECTIVE: Adequate fruit and vegetable intake has been suggested to protect against colorectal cancer. However, several recent prospective studies have reported no association. We therefore examined the association between fruit and vegetable intakes and the risk of colorectal cancer in a prospective cohort study in Japan. DESIGN: Between June and August 1990, 47 605 Japanese men and women completed a self-administered questionnaire, including a food-frequency questionnaire. We divided the subjects into quartiles based on their self-reported fruit and vegetable consumption. There were 165 colon cancer and 110 rectal cancer incidences identified during 7 years of follow-up, to the end of December 1997. We used Cox proportional hazards models to estimate the relative risk (RR) of developing colorectal cancer according to the level of fruit and vegetable consumption, applying adjustments for potential confounders. RESULTS: No statistically significant association was observed between fruit and vegetable consumption and the risk of colorectal cancer. The multivariate RR of colon cancer in the highest quartile of fruit and vegetable intake compared with the lowest was 1.13 (95% confidence interval (CI) 0.73-1.75), the RR for vegetables alone was 1.24 (95% CI 0.79-1.95) and that for fruit alone was 1.45 (95% CI 0.85-2.47). The corresponding multivariate RRs for rectal cancer were 1.12 (95% CI 0.67-1.89), 1.14 (95% CI 0.67-1.93) and 1.41 (95% CI 0.73-2.73). CONCLUSIONS: We found no association between the consumption of fruit and vegetables and the risk of colorectal cancer among the Japanese population. 相似文献