不同激素处理对厚荚相思组培苗生根及移栽效应

以改良MS为基本培养基,使用不同浓度的NAA、IAA、ABT、IBA和MET对厚荚相思无菌苗进行生根诱导,生根苗按使用不同的激素处理分别进行移栽试验.厚荚相思生根诱导率为11%～100%,其中以改良MS NAA1.0 mg/L的生根率最高,达100%;以黄心土为基质对生根苗进行移栽,移栽成活率为41.25%～81.79%,其中成活率最高为使用NAA诱导生根的生根苗,成活率达81.79%.     

渥丹百合农艺性状及活性成分对钾元素的响应

为探明钾素与渥丹百合生长发育及鳞茎活性成分积累的关系,采用Hoagland营养液浇灌技术,研究不同钾水平(K0、K1、K2、K3、K4)对渥丹百合株高、茎粗、叶片数、叶绿素含量的影响,采用分光光度法、火焰光度法测定并分析了鳞茎中活性物质(多糖、总酚、总黄酮、皂苷)及钾含量的积累.结果表明:1)渥丹百合农艺性状对钾水平的...     

First record of avian Plasmodium DNA from mosquitoes collected in the Yaeyama Archipelago, southwestern border of Japan

We studied the prevalence of avian Plasmodium in 509 mosquitoes of 9 species collected from the Ishigaki and Iriomote islands in the Yaeyama Archipelago, located southwest from the mainland of Japan. Two identical avian Plasmodium lineages were detected from Culex (Culiciomyia) nigropunctatus. Detected lineages were phylogenetically classified into different clade to avian Plasmodium lineages from birds and mosquitoes in the mainland of Japan but identical to a lineage detected from a resident bird, White-breasted Waterken (Amaurornis phoenicurus). This is the first detection of avian Plasmodium DNA from mosquitoes in the Yaeyama Archipelago and suggested that resident birds might have been infected with an avian Plasmodium lineage specific to the studied area and C. nigropunctatus could be the candidate vector mosquito species.     

机械深松技术探讨

介绍机械深松的作用及运城市几种常用深松机的使用情况;探讨机械深松的有关技术问题.机械深松具有广泛的地域适应性和作物适应性,是一项低投入、高产出,集经济、社会、生态效益于一体的耕作技术措施,应大力推广应用.     

M、F型COII基因在雌雄三角帆蚌不同发育时期的表达差异研究

与传统的母系遗传不同,双壳贝类线粒体有M和F型2种mt DNA,称为双单亲遗传(doubly uniparental inheritance,DUI)。为了探究DUI的形成机制,实验利用RACE方法得到三角帆蚌M、F-type COⅡ基因的cDNA全长;荧光定量检测各时期各组织中M、F-type COⅡ基因的表达。结果发现,(1)M-type COⅡ基因的cDNA序列全长1244 bp,F-type COⅡ基因的cDNA序列全长808 bp,M型3′端较F型有一段546 bp的特异性延长序列;跨膜结构预测显示M-type COⅡ基因3′特异性延伸区域有4个跨膜结构,推测这段延伸序列具有一定的功能性;(2)早期幼龄(胚胎期-5月龄)三角帆蚌的组织团中,M、F-type COⅡ基因均能检测出,且同时期中F-type COⅡ基因的表达量显著高于M-type COⅡ;(3)6月龄蚌各组织中,F-type COⅡ基因在各组织中表达量明显高于M型的表达量,而M-type COⅡ基因在性腺中表达明显高于其他组织;(4)一龄雌性各组织中,M、F-type COⅡ基因均有表达;而一龄雄性各组织中,M-type COⅡ基因仅在性腺中表达;(5)二龄雌性各组织中,M、F-type COⅡ基因均有表达;二龄雄性各组织中,F-type COⅡ基因除性腺外的其他组织中均有低量表达,M-type COⅡ基因仅在性腺中表达,且表达量较高。研究表明,在三角帆蚌雌雄个体发育过程中,M-type mt DNA在组织中选择性降解或聚集。     

Effect of Bacillus subtilis spore administration on activation of macrophages and natural killer cells in mice

The effect of Bacillus subtilis (strain A102) spores on the activation of murine macrophages and natural killer cells (NK) was examined. The macrophage activity and NK activity were enhanced by oral administration of A102 spores, and slightly enhanced by oral administration of culture supernatant. There was no difference in the results of macrophage activity and NK activity using other live or dead spores. The NK activity and macrophage activity were increased with increments of concentration up to 0.1 g per mouse, and both activities were decreased at concentration of more than 0.15 g per mouse. The NK activity was increased 1 and 2 days after oral administration of A102 spores, and the activity level 2 days after administration was about 3-fold higher than the level prior to treatment. Macrophage activity was also increased from 1 to 3 days after oral administration of A102 spores, and the activity level 3 days after administration was about 3-fold higher than the level prior to treatment. The induction of interferons at 1 day after oral administration in mouse serum was 5-fold higher than that in controls. These findings indicate that oral administration of A102 gave rise to the induction of interferons, and it is likely that macrophages and NK cells were activated by interferons.     

抗冻蛋白在园艺植物遗传改良中的研究进展

低温冻害是园艺植物生产中的严重自然灾害,直接影响其地域的分布、品质、产量和经济效益.同时由于地理环境的差异,园艺植物的分布呈现一定的区域性和季节性.低温胁迫常使某些起源于热带、亚热带的园艺植物因其冷敏感性而无法在温带以北的地区露地安全越冬,大大限制了其种植范围,同时在引种过程中也会造成巨大的经济损失,因此园艺植物抗冻性的研究已成为该领域的研究热点之一.园艺植物产值高,具有观赏性和丰厚的经济价值,前人曾用常规育种方法改良园艺植物的遗传特性,使之避免冻害损伤以扩大种植范围,就目前来看,很难提高其抗冻性.近年来,随着分子生物学的发展,动、植物抗寒、抗冻机理的认识不断加深,基因工程技术已经成为培育抗冻性园艺植物新品种最有效的途径.本文旨在论述抗冻蛋白在园艺植物中的研究进展,为园艺植物的抗冻遗传改良提供借鉴.     

低能N+辐照提高小麦反转录转座子Wis 2-1A的转录并影响其邻近基因表达

为研究小麦反转录转座子Wis2-1A在低能离子束辐照小麦生物效应中的作用,用实时定量PCR的方法测定不同注量下Wis2-1A的转录水平及在基因组中的拷贝,并用转座子显示技术研究了Wis2-1A在注量为5×1017N+/cm2的低能离子束处理后的转录对其邻近基因表达的影响,结果显示:不同注量的N+辐照都能提高Wis2-1A的转录水平,但在基因组水平上的拷贝没有大的提高,且在较高注量下,由于Wis2-1A的高水平转录导致其邻近的某些基因沉默,这说明Wis2-1A在低能离子注入生物效应中起主要作用。