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31.
Naoki SASAKI Yoshinori MORITA Tomoe MORIYAMA Haruo YAMADA 《Journal of Equine Science》2009,20(2):19-22
The purpose of this study was to evaluate the effect of the canine cranial cruciate
ligament repair system on laryngeal hemiplegia in heavy draft horses. Twenty-four heavy
draft horses diagnosed with grade 4 laryngeal hemiplegia were allocated to either the
prosthetic laryngoplasty (PL) group (n=14) or a canine cranial cruciate ligament repair
system (CCCLRS) group (n=10). Right to left angle quotients (RLQs) of abductions of the
arytenoid cartilages were endoscopically evaluated before and after surgery.
Post-operative RLQs in the CCCLRS group were significantly lower than those of the PL
group (P<0.01). The canine cranial cruciate ligament repair system was revealed to be a
good surgical procedure for laryngeal hemiplegia in heavy draft horses. 相似文献
32.
Takahisa YAMADA Seiki SASAKI Shin SUKEGAWA Takeshi MIYAKE Tatsuo FUJITA Hiroyuki KOSE Mitsuo MORITA Youichi TAKAHAGI Hiroshi MURAKAMI Fumiki MORIMATSU Yoshiyuki SASAKI 《Animal Science Journal》2009,80(4):486-489
Marbling, defined by the amount and distribution of intramuscular fat, is an economically important trait of beef cattle in Japan. The endothelial differentiation , sphingolipid G-protein-coupled receptor , 1 ( EDG1 ) gene has been considered as a positional functional candidate for the gene responsible for marbling. We have recently reported that 2 single nucleotide polymorphisms (SNPs), c.-312A > G in the 5' untranslated region (UTR) and c.*446G > A in the 3' UTR in EDG1 were associated with marbling in Japanese Black beef cattle, but this was not functional and a causal mutation for marbling. In the present study, we detected 2 novel SNPs, referred to as g.1475435G > A and g.1471620G > T , in the 5' flanking region of the EDG1 between low-marbled and high-marbled steer groups, which were previously shown to have EDG1 expression differences in musculus longissimus muscle. The g.1475435G > A SNP seemed not to segregate in Japanese Black beef cattle. The g.1471620G > T SNP was associated with the predicted breeding value for beef marbling standard number by the analyses using Japanese Black beef cattle population. Based on these findings, we hypothesized that the g.1471620G > T SNP might have an impact on EDG1 expression and also marbling. 相似文献
33.
Hepatic AA amyloidosis in a cat: cytologic and histologic identification of AA amyloid in macrophages 下载免费PDF全文
Sakurako Neo‐suzuki Takayuki Mineshige Junichi Kamiie Hideki Kayanuma Yukako Mochizuki Masaharu Hisasue Ryo Tsuchiya Kinji Shirota 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2017,46(2):331-336
A 3‐year‐old, spayed female, Domestic Shorthair cat presented with anorexia, lethargy, vomiting, probable hemoabdomen, and multiple masses on the right lateral liver lobe. Clinicopathologic and imaging abnormalities included anemia, azotemia, icterus, and hepatomegaly with hypoechoic masses. On cytologic evaluation of a fine‐needle aspiration of a liver mass there was abundant extracellular pink‐ to purple‐colored material between hepatocytes. The amorphous material was stained with direct fast scarlet (DFS), and green birefringent areas were observed under polarized light, confirming the presence of amyloid. A unique finding on the cytologic smear were macrophages containing amorphous and fibrillar amyloid‐like protein. Histopathologic examination using H&E and Congo red staining confirmed amyloid deposits within the space of Disse, along the sinusoids, portal tracts, blood vessel walls, and within the cytoplasm of macrophages. Immunohistochemical staining with anti‐AA amyloid antibodies further confirmed the presence of AA amyloid. To the author's knowledge, this is the first report of the cytologic finding of AA amyloid protein within macrophages and DFS stain detection of amyloid on a cytologic smear. 相似文献
34.
Fumie MAGATA Mitsuo ISHII Eri OIKAWA Hidefumi FURUOKA Kazutaka YAMADA Naoki SASAKI Syo SHIMIZU Hisashi INOKUMA 《Journal of Equine Science》2010,21(2):17-20
A 42-day-old heavy draft horse fell into sudden astasia. Significant swelling and heat
sensation of the left femoral region were observed. Because of a friction sound in the
left hip, we supposed that the hip joint was dislocated or the hip bone was fractured.
Computed Tomography (CT) examination showed that the left hip joint was dislocated and the
left femoral head was disjunct. We carried out a pathological autopsy, and made a
diagnosis of the foal as fracture of the hip bone and femoral head with suppurative
umbilical arteritis. Pathologic changes in the umbilical artery and hind leg were
completely unilateral, suggesting that left umbilical arteritis spread to the blood
circulation, causing arthritis and dislocation of the hip bone. 相似文献
35.
Naoto WATANABE Takahisa YAMADA Sachiyo YOSHIOKA Masayuki ITOH Youichi SATOH Masako FURUTA Shigeki KOMATSU Yoshihiko SUMIO Tatsuo FUJITA Yoshiyuki SASAKI 《Animal Science Journal》2010,81(1):142-144
Our previous study detected a single nucleotide polymorphism (SNP), g.1471620G > T , in the 5' flanking region of the endothelial differentiation sphingolipid G-protein-coupled receptor 1 ( EDG1 ) gene, which has been considered as a positional functional candidate for the gene responsible for marbling, and showed association of the g.1471620G > T SNP with marbling in Japanese Black beef cattle. In the present study, we investigated the allele frequency distribution of the g.1471620G > T SNP among the 5 cattle breeds, Japanese Black, Japanese Brown, Japanese Short Horn, Holstein, and Brown Swiss breeds. The T allele at the g.1471620G > T SNP associated with high marbling was found at high frequency in Japanese Black breed that has been subjected to a strong selection for high marbling, while the allele was absent or at very low frequencies in the other breeds that have not been strongly selected for high marbling. Based on this finding, we hypothesized that the pressure of the strong selection for high marbling in Japanese Black breed has increased the frequency of the T allele at the g.1471620G > T SNP in the EDG1 . 相似文献
36.
Koichi CHIKUNI Mika OE Keisuke SASAKI Masahiro SHIBATA Ikuyo NAKAJIMA Koichi OJIMA Susumu MUROYA 《Animal Science Journal》2010,81(5):600-605
To assess the role of muscle fiber type in beef taste‐traits, we analyzed cooked meats from bovine masseter, diaphragm, psoas major, longissimus thoracis, and semitendinosus muscles with an electric taste sensing system (INSENT SA402B). The system is composed of five taste sensors of polymer membranes fixing different lipids. The sensors, CT0, CA0, AAE, C00 and AE1 are designed to respond to the individual tastes of salty, sour, umami, bitter and astringent, respectively. The system found significant differences in the converted outputs of CA0 (cvCA0), C00 (cvC00) and AE1 (cvAE1) among the bovine muscles. The slow‐type muscles (masseter and diaphragm) showed lower cvCA0, higher cvC00, and higher cvAE1 than did the fast‐type muscles (psoas major, longissimus thoracis, and semitendinosus). Lactic acid content was different among muscle types and was highly related to the cvCA0 output and pH. carbonyl compounds and free fatty acids were higher in the slow‐type muscles. Free fatty acids were major components causing the difference in the C00 output among the muscle types. Iron content was also different among the muscle types and related to the cvC00 and cvAE1 outputs. These results suggested that the muscle fiber type affects the beef taste characteristics. 相似文献
37.
Osamu SASAKI Mitsuo AIHARA Koichi HAGIYA Akiko NISHIURA Kazuo ISHII Masahiro SATOH 《Animal Science Journal》2012,83(2):95-102
The objective of this study was to confirm the stability of the genetic estimation of longevity of the Holstein population in Japan. Data on the first 10 lactation periods were obtained from the Livestock Improvement Association of Japan. Longevity was defined as the number of days from first calving until culling or censoring. DATA1 and DATA2 included the survival records for the periods 1991–2003 and 1991–2005, respectively. The proportional hazard model included the effects of the region-parity-lactation stage-milk yield class, age at first calving, the herd–year–season, and sire. The heritabilities on an original scale of DATA1 and DATA2 were 0.119 and 0.123, respectively. The estimated transmitting abilities (ETAs) of young sires in DATA1 may have been underestimated, but coefficient δ, which indicated the bias of genetic trend between DATA1 and DATA2, was not significant. The regression coefficient of ETAs between DATA1 and DATA2 was very close to 1. The proportional hazard model could steadily estimate the ETA for longevity of the sires in Japan. 相似文献
38.
不同地下水位胡杨蒸腾速率与叶水势的变化分析 总被引:3,自引:0,他引:3
本文研究了不同地下水位胡杨蒸腾速率、叶水势及蒸腾速率的季节变化等水分生理特性,结果表明:地下水位影响胡杨蒸腾作用,同时土壤水分状况也影响蒸腾速率,当地下水位在2.28~2.43 m时,胡杨蒸腾速率变化幅度大,蒸腾速率的日最大值为7.50 mmol.m-2s-1,日均值为4.73 mmol.m-2s-1,对应叶水势的日最低值为-3.55(Mpa)和日均值为-1.94(Mpa);地下水位2.91~3.33 m时,叶水势的日最低值为-2.61(Mpa)和日均值为-1.62(Mpa)。胡杨蒸腾速率季节变化在7月份达到最大值,靠近河岸胡杨蒸腾速率变化较远离河岸胡杨大,表明地下水位低时,胡杨保持较高的水势就能从环境中吸收到水分来满足其正常生长。 相似文献
39.
Takai S Zhuang D Huo XW Madarame H Gao MH Tan ZT Gao SC Yan LJ Guo CM Zhou XF Hatori F Sasaki Y Kakuda T Tsubaki S 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(7):739-742
Little is known about the distribution of Rhodococcus equi in the soil environment of native horses in China. One hundred and eight soil samples were collected from native-horse farms in the Hulun Beier grasslands of eastern Mongolia, the Xilin Goler grasslands of southern Mongolia, and Tongliao City in Inner Mongolia, China. The isolation rates of R. equi from soil samples from the Hulun Beier and Xilin Goler grasslands ranged from 25.9% to 30.0%. In contrast, isolation rates from soil samples from Tongliao City were as high as 82.3% and the mean number of R. equi in soil samples from Tongliao City was 10 times more than those of samples from the grasslands. The 488 isolates were examined using PCR for the presence of genes that encode virulence-associated 15-17 kDa antigen protein (VapA) and the 20 kDa antigen protein (VapB). All isolates were negative for virulence-associated proteins. Plasmid profiles of these avirulent isolates showed that cryptic plasmids of various sizes were present with an incidence of 13.3% to 21.5%. The results of the present study contrast with those of our recent study (J. Vet. Med. Sci. 67:611-613, 2005), in which we reported that R. equi was absent from Mongolian horses in Ulaanbaatar, Mongolia. It is suggested that the difference between the results of these two studies is due to the mobile pasturing system in Mongolia and nonmobile pasturing system in Inner Mongolia. 相似文献
40.
Sang-gun ROH Yeon-hee HONG Daisuke HISHIKAWA Hiroaki TSUZUKI Hisae MIYAHARA Yukihiko NISHIMURA Chizu GOTO Yasuki SUZUKI Ki-choon CHOI Hong-gu LEE Shinichi SASAKI 《Animal Science Journal》2005,76(4):381-386
Ghrelin and growth hormone secretagogues receptor (GHS‐R or ghrelin receptor) have been reported as being one of the factors of adipogenesis in adipocytes. To investigate the involvement of ghrelin and GHS‐R in adipocytes, the effect of the GHS‐R antagonist, [D‐Lys‐3]‐GHRP‐6 (His‐D‐Trp‐D‐Lys‐Trp‐D‐Phe‐Lys‐NH2), on the process of adipogenesis in ovine and rat adipocytes was evaluated. [D‐Lys‐3]‐GHRP‐6 (10?7 mol/L) significantly inhibited adipogenic differentiation of ovine and rat preadipocytes prepared from adipose tissues. The level of peroxisome proliferator activated receptor (PPAR)‐γ2 mRNA, an adipogenic marker, was decreased during the differentiation of adipocytes treated with [D‐Lys‐3]‐GHRP‐6 for 10 days. Ghrelin stimulated adipogenesis, also causing an increment of glycerol‐3‐phosphate dehydrogenase and upregulation of PPAR‐γ2. Furthermore, the antilipolytic effect of ghrelin was attenuated by treatment with [D‐Lys‐3]‐GHRP‐6 in both types of isolated adipocytes. Overall, the results of the present study highlight that GHS‐R in adipogenesis can be blocked by treatment with [D‐Lys‐3]‐GHRP‐6. 相似文献