全文获取类型
收费全文 | 553篇 |
免费 | 19篇 |
国内免费 | 20篇 |
专业分类
林业 | 126篇 |
农学 | 15篇 |
基础科学 | 8篇 |
79篇 | |
综合类 | 38篇 |
农作物 | 33篇 |
水产渔业 | 56篇 |
畜牧兽医 | 172篇 |
园艺 | 20篇 |
植物保护 | 45篇 |
出版年
2024年 | 2篇 |
2023年 | 6篇 |
2022年 | 34篇 |
2021年 | 27篇 |
2020年 | 18篇 |
2019年 | 14篇 |
2018年 | 16篇 |
2017年 | 15篇 |
2016年 | 12篇 |
2015年 | 21篇 |
2014年 | 27篇 |
2013年 | 32篇 |
2012年 | 35篇 |
2011年 | 38篇 |
2010年 | 15篇 |
2009年 | 17篇 |
2008年 | 29篇 |
2007年 | 48篇 |
2006年 | 23篇 |
2005年 | 32篇 |
2004年 | 25篇 |
2003年 | 25篇 |
2002年 | 11篇 |
2001年 | 17篇 |
2000年 | 15篇 |
1999年 | 5篇 |
1998年 | 14篇 |
1997年 | 4篇 |
1996年 | 6篇 |
1995年 | 1篇 |
1992年 | 2篇 |
1991年 | 1篇 |
1987年 | 1篇 |
1985年 | 1篇 |
1975年 | 1篇 |
1974年 | 1篇 |
1973年 | 1篇 |
排序方式: 共有592条查询结果,搜索用时 15 毫秒
81.
东北坡耕地春季融雪侵蚀观测研究 总被引:5,自引:4,他引:1
为研究东北地区坡耕地的春季融雪侵蚀特征及其影响因素,选取吉林省吉兴小流域内坡耕地进行原位观测,通过分析融雪过程中径流量和含沙量的变化,以及融雪径流、土壤解冻深度等指标对融雪侵蚀的影响,探讨坡耕地融雪侵蚀过程及变化规律。结果表明,在日平均温度0~3.8℃的气象条件下,春季融雪侵蚀较为集中,径流与含沙量变化均先增加后减少。融雪径流与表层土壤解冻深度是影响融雪侵蚀的重要因素,初期融雪产流,土壤未解冻,径流急剧增加,径流量占融雪期总径流量的59.15%;中期积雪融化趋于稳定,土壤表层开始解冻,径流减少含沙量增加,侵蚀量达到最大且占融雪期总侵蚀量的41.74%;末期融雪产流停止,土壤解冻深度增加,含沙量达到最大(8.00kg/m~3)。坡耕地融雪侵蚀受垄作区域与集水洼地地形变化的影响,产流产沙具有较强规律性,二者峰值出现频次一致时,径流—泥沙呈"8"字循环滞后关系,反之呈复式循环滞后关系。 相似文献
82.
Improving the antibacterial activity against Staphylococcus aureus of composite sheets containing wasted tea leaves by roasting 总被引:1,自引:0,他引:1
We used various kinds of wasted tea leaves to develop composite sheets with antibacterial properties. Antibacterial tests
showed that the number of viable bacterial cells for the sheet containing wasted green tea leaves was around 10:6–107 CFU/ml after 18 h culture compared to 108 CFU/ml for a tea-free sheet. This indicates that cell growth was signifi cantly
inhibited. For sheets containing other types of tea leaves (oolong, black, hojicha, and pu-erh), living cells were not found, indicating that these sheets had superior antibacterial effects. Living cells were also not
found in sheets containing wasted black tea leaves or roasted tea leaves at a concentration of 60% by weight after 6 h cultivation.
Therefore, roasting treatment of wasted green tea leaves was examined to improve the antibacterial activity of the sheet.
In particular, the focus was on structural conversion of catechins by heating. 相似文献
83.
Masako Kubo Koji Shimano Hitoshi Sakio Yuji Isagi Keiichi Ohno 《Journal of Forest Research》2010,15(5):337-340
Cercidiphyllum japonicum and C. magnificum are deciduous tree species that produce large numbers of sprouts. They are found mainly in riparian and/or disturbed areas.
C. japonicum is distributed in the montane zone in Japan, whereas C. magnificum is distributed mostly in the subalpine zone of central Japan. However, the two species sometimes coexist, e.g., on the talus
slope at the valley head of the Chichibu Mountains. We investigated differences in sprouting traits in these two species by
comparing class distributions of sprout diameter at breast height (DBH) and heights of individuals on the talus slope. Sprout
DBH and individual height were smaller in C. magnificum as compared to C. japonicum. Moreover, the analysis of the DBH-class distribution of each species indicated that C. magnificum had numerous small sprouts and experienced high mortality, whereas C. japonicum had fewer small sprouts and low mortality. It is likely that the sprouting traits of C. magnificum make it more adapted to severe conditions in the subalpine zone than those of C. japonicum. 相似文献
84.
Murakami Y Okazaki Y Okayama S Fujihira S Noto T Nakatsuji S Oishi Y 《Journal of toxicologic pathology》2010,23(2):85-89
We report here the interesting case of a 5-year-old male cynomolgus monkey with goblet cell hyperplasia and thickening of the muscular layer throughout the small intestine without exhibiting any clinical symptoms. Necropsy examination showed diffuse thickening of the intestinal wall from the jejunum to the ileum, with an appearance likened to a rubber tube. Histopathologically, marked thickening was observed in both the mucosal and muscular layers in the jejunum and ileum, and slight thickening was observed in the duodenum. Goblet cell hyperplasia with extension of the circular folds and villi was prominently observed. The mucosal surface was covered with a thick mucus layer containing desquamated mucosal epithelial cells, and both the inner and outer muscular layers were markedly thickened due to smooth muscle hypertrophy. Neither macroscopic nor histopathological examination identified any causative factors, such as infection, enteritis and intestinal stenosis, or obstruction that may have caused development of this lesion. Given these observations, this case may simply be considered of spontaneous goblet cell hyperplasia and muscular layer thickening in the small intestine of a cynomolgus monkey. 相似文献
85.
Keitaro Ohmori Akane Tanaka Yuka Makita Masaki Takai Yuji Yoshinari Hiroshi Matsuda 《Veterinary dermatology》2010,21(5):477-483
Ultrapure soft water (UPSW) is water in which calcium and magnesium ions have been replaced with sodium ions using a cation‐exchange resin. We recently demonstrated that washing with soap and UPSW reduced the clinical severity of dermatitis and improved the skin barrier function in NC/NgaTnd mice, a murine model for human atopic dermatitis. The purpose of this pilot study was to evaluate the efficacy of shampoo treatment with UPSW for dogs with pruritus. Eleven dogs with pruritus were randomly assigned to two groups depending on whether they received weekly shampoo treatment with UPSW or tap water for 4 weeks. After a washout period, the treatment protocol was switched such that each dog received both treatments. The pre‐treatment and post‐treatment values of the following were compared: pruritus scores assessed by the owners; dermatitis scores recorded by an investigator; and transepidermal water loss (TEWL). Shampoo treatment with UPSW significantly decreased pruritus and dermatitis scores in the dogs, whereas shampoo treatment with tap water did not. In addition, shampoo treatment with UPSW, but not with tap water, significantly reduced TEWL in the dogs. Adverse events due to the treatment were not observed in the dogs. Furthermore, we found that topical application of UPSW for barrier‐disrupted skin caused by tape stripping in healthy dogs decreased TEWL more rapidly than topical application of tap water. Our findings suggest that shampoo treatment with UPSW promotes skin barrier recovery and thus could be considered as a possible therapeutic option in the management of pruritus and dermatitis in dogs. 相似文献
86.
J Qiu Y Kitamura Y Miyata S Tamaru K Tanaka T Tanaka T Matsui 《Journal of agricultural and food chemistry》2012,60(32):8036-8043
The aim of this study is to illustrate the in vivo and in vitro absorption of theasinensins B and A that are (-)-epigallocatechin-3-O-gallate (EGCG)-(-)-epigallocatechin (EGC) dimer and EGCG dimer, respectively, and their transport pathway across the intestinal membrane. Our animal study by a single oral administration to rats demonstrated the intact absorption of theasinensins into the blood system, which was estimated to be a >10-fold lower absorption amount than EGCG. The in vitro absorption study indicated that theasinensins can be transported across Caco-2 cell monolayers, while their permeability coefficients were also >10-fold lower than those of EGCG and EGC. Transport experiments using cytochalasin D or quercetin as a tight junction (TJ) modulator and a non-saturable permeation revealed that theasinensins were transported across Caco-2 cells in a TJ paracellular diffusion route. In conclusion, the dimers of condensed catechins, theasinensins B and A, can be absorbed intact into rat blood and transported across Caco-2 cell monolayers probably through a TJ paracellular pathway. 相似文献
87.
Cloned porcine embryos can maintain developmental ability after cryopreservation at the morula stage
Nakano K Matsunari H Nakayama N Ogawa B Kurome M Takahashi M Matsumoto M Murakami H Kaji Y Nagashima H 《The Journal of reproduction and development》2011,57(2):312-316
The aim of the present study was to clarify the overall efficiency of porcine somatic cell nuclear transfer (SCNT) by incorporating cryopreservation of the cloned embryos before transfer. The SCNT embryos reconstructed with preadipocytes and in vitro-matured (IVM) oocytes were cultured to harvest morula stage embryos; they were then subjected to delipation (removal of cytoplasmic lipid droplets) and vitrification. After warming and culture, the embryos developing to blastocysts were transferred to recipients to obtain cloned piglets. From 372 reconstructed embryos, 188 (50.5%) reached the morula stage and 117 (31.5%) developed to blastocysts after vitrification. Transfer of 98 (26.3%) morphologically normal blastocysts gave rise to 6 (1.6%) piglets, including 1 stillborn. The efficiency of the cloned piglet production was comparable with that obtained using SCNT embryos without cryopreservation (2.7%, 17/635). Here, we demonstrate that porcine somatic cell cloning can be performed without a significant reduction in efficiency even when the SCNT embryos are cryopreserved before transfer. 相似文献
88.
This study assessed the effects of cryoprotectant concentration during equilibration on the efficiency of bovine blastocyst vitrification and the expression of selected developmentally important genes. In vitro produced bovine blastocysts were equilibrated in either 7.5% ethylene glycol (EG) + 7.5% DMSO (Va group) or in 2% EG + 2% DMSO (Vb group) then vitrified on Cryotop® sheets in 16.5% EG + 16.5% DMSO + 0.5M sucrose. After warming, embryos were cultured for 48 hr. Re‐expansion, hatching, and the numbers of total and membrane damaged cells were compared among vitrified groups and a control. There was no significant difference between the vitrified groups in survival, cell numbers and the extent of membrane damage. Vitrification increased the number of membrane‐damaged cells in both groups, however, in a greater extent in the Vb group. Vitrification increased (p < .05) the expression of the HSP70 gene in Va but not in Vb embryos. The expression of IGF2R, SNRPN, HDAC1, DNMT3B, BAX, OCT4, and IFN‐t genes were the same in control and vitrified groups. In conclusion, the concentration of cryoprotectants during equilibration did not affect survival rates; however, normal cell numbers could be maintained only by equilibration in 15% cryoprotectants which was associated with increased HSP70 expression. 相似文献
89.
Tomoyuki Makino Syuichi Hasegawa Yasuhiro Sakurai Satoshi Ohno Hirokatsu Utagawa Yuji Maejima 《Soil Science and Plant Nutrition》2013,59(3):581-590
Abstract The changes in the exchangeable Mn (Ex-Mn), exchangeable Co (Ex-Co), and exchangeable Cu (Ex-Cu) contents by air-drying of soils were reported in our previous paper. The drying effect, however, was not analyzed in the field. The objective of this study was to investigate the influence of soil-drying under field conditions on Ex-Mn, Ex-Co, and Ex-Cu levels. Two paddy soils (Tsukuba and Yawara) were collected at four different soil depths (0?1, 1?5, 5?10, and 10?20 cm) under three different soil moisture conditions i.e., after harvest, after successive sunny days and after rainy days. After sunny days, Ex-Mn and Ex-Co contents in the surface soil layer markedly increased and drastically decreased after rainfall, while they remained almost unchanged in the lower soil layers. Ex-Cu contents slightly increased in the surface soil layer after sunny days. A similar tendency was observed in soil column experiments. The contents of Ex-Mn, Ex-Co, and Ex-Cu increased 12, 12, and 2 times in the upper layer of soil columns that had been sampled from the Yawara field and stored in a dark room for 8 d. The increase of the Ex-Mn and Ex-Co contents in the upper layer could not be explained by the capillary rise of soil water from the lower layers of the columns. In a soil-drying experiment under laboratory conditions, Ex-Mn and Ex-Co contents began to increase when the water content of the Yawara soil was lower than 0.09?0.13 kg kg?1. The water content of the surface soil of the Yawara field after sunny days was 0.08 kg kg?1. Ex-Cu content did not change apprecially with the water content. It is concluded that the increase of the Ex-Mn and Ex-Co levels in field surface soils after sunny days can be ascribed to soil-drying. Some of the excess Ex-Mn and Ex-Co is expected to leach down into lower layers due to rain, which may contribute to plant uptake of Mn and Co. 相似文献
90.
为进一步开展欧洲输华粮谷中有害生物风险分析,该文整理和统计2003—2017年我国自欧洲输华粮谷中截获的有害生物数据,并采用SPSS 20软件分别对所截获的主要杂草和昆虫进行了聚类分析、相关分析和主成分分析,确定其截获次数的主控因子。结果显示,我国自欧洲输华粮谷中截获的主要有害生物为杂草,截获记录数为7 905次,占总截获记录的79.99%;我国自欧洲输华大麦中截获的有害生物次数均最多,截获次数为57 843次,占总截获次数的51.39%;我国自乌克兰输华粮谷中截获的杂草和昆虫次数最多,分别为39 026次和678次;我国自欧洲输华粮谷中截获的杂草和昆虫分别为479种和61种;自乌克兰和大麦中截获的杂草和昆虫的Shannon-Weiner指数均相对较高;我国自欧洲输华大麦和玉米中截获的杂草和昆虫分别单独聚为一类,我国自乌克兰和法国输华粮谷中截获的杂草和昆虫分别单独聚为一类;欧洲输华粮谷贸易额是有害生物杂草和昆虫截获次数的主控因子。建议我国进一步做好有害生物截获数据收集工作,加强欧洲输华粮谷中有害生物风险分析,根据风险对不同国家和粮谷采取相应的检疫措施。 相似文献