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31.
32.
Non-native pollinator species are now widely utilized to facilitate pollination of agricultural crops. Evaluation of the ecological risk of alien pollinators is necessary because they could have a large impact on native ecosystems through disturbing native plant-pollinator interactions. We conducted a greenhouse experiment to examine the impact of the non-native commercialized European bumblebees, Bombus terrestris, on the pollination success of seven Japanese bumblebee-pollinated plant species. Plants were exposed to three groups of bumblebees: native bumblebee(s) only (NATIVE treatment); the alien bee only (ALIEN) and a mix of the two (MIX). ALIEN treatment had negative effects on fruitset and/or fruit quality of five plants, including self-incompatible and compatible, herb and woody, and queen- and worker-pollinated species. The negative effects were caused by a decrease in legitimate flower visitation due to (1) physical inaccessibility to nectary in deep-corolla flowers by the alien bee with insufficient tongue length and, (2) biased flower preference between short-corolla flowers. Fruitset tended to decrease drastically for the self-incompatible species while fruit quality decreased moderately for the self-compatible species. Effects of MIX were not intermediate between NATIVE and ALIEN in most plant species, and caused pollination success to vary in an unpredictable manner amongst plant species, probably due to interaction between native and alien bees. This non-linear relationship between plants’ pollination success and the relative density of the alien suggests that the alien bee can disturb pollination of a plant species even when only representing a small fraction of the total pollinator community. 相似文献
33.
Katsuyuki Hayakawa Keiko Tanaka Toshiki Nakamura Shigeru Endo Tsuguhiro Hoshino 《Cereal Chemistry》1997,74(5):576-580
The viscoelastic properties and molecular structure of the starch isolated from waxy (amylose-free) hexaploid wheat (WHW) (Triticum aestivum L.) were examined. WHW starch generally had lower gelatinization onset temperature, peak viscosity, and setback than the starch isolated from normal hexaploid wheat (NHW). Differential scanning calorimetry (DSC) showed that WHW starch had higher transition temperatures (To, Tp, and Tc) and enthalpy (ΔH) than NHW starch. However, when compared on the basis of amylopectin (AP) content, ΔH of WHW starch was almost statistically identical to that of its parental varieties. Typical A-type X-ray diffraction patterns were observed for the starches of WHW and its parental varieties. Somewhat higher crystallinity was indicated for WHW starch. WHW starch was also characterized by having greater retrogradation resistance. The high-performance size-exclusion chromatography (HPSEC) of amylopectin showed that each amylopectin yielded two fractions after debranching. Although WHW amylopectin had somewhat long B chains, little difference was observed in the ratio of Fr.III/ Fr.II between WHW and its parental varieties. 相似文献
34.
Igakura T Stinchcombe JC Goon PK Taylor GP Weber JN Griffiths GM Tanaka Y Osame M Bangham CR 《Science (New York, N.Y.)》2003,299(5613):1713-1716
Cell contact is required for efficient transmission of human T cell leukemia virus- type 1 (HTLV-I) between cells and between individuals, because naturally infected lymphocytes produce virtually no cell-free infectious HTLV-I particles. However, the mechanism of cell-to-cell spread of HTLV-I is not understood. We show here that cell contact rapidly induces polarization of the cytoskeleton of the infected cell to the cell-cell junction. HTLV-I core (Gag protein) complexes and the HTLV-I genome accumulate at the cell-cell junction and are then transferred to the uninfected cell. Other lymphotropic viruses, such as HIV-1, may similarly subvert normal T cell physiology to allow efficient propagation between cells. 相似文献
35.
Nobuko Katayanagi Yuichiro Furukawa Tamon Fumoto 《Soil Science and Plant Nutrition》2013,59(3):360-372
The DNDC (DeNitrification-DeComposition)-Rice model, one of the most advanced process-based models for the estimation of greenhouse gas emissions from paddy fields, has been discussed mostly in terms of the reproducibility of observed methane (CH4) emissions from Japanese rice paddies, but the model has not yet been validated for tropical rice paddies under alternate wetting and drying (AWD) irrigation management, a water-saving technique. We validated the model by using CH4 and nitrous oxide (N2O) flux data from rice in pots cultivated under AWD irrigation management in a screen-house at the International Rice Research Institute (Los Baños, the Philippines). After minor modification and adjustment of the model to the experimental irrigation conditions, we calculated grain yield and straw production. The observed mean daily CH4 fluxes from the continuous flooding (CF) and AWD pots were 4.49 and 1.22?kg?C?ha?1?day?1, respectively, and the observed mean daily N2O fluxes from the pots were 0.105 and 34.1?g?N?ha?1?day?1, respectively. The root-mean-square errors, indicators of simulation error, of daily CH4 fluxes from CF and AWD pots were calculated as 1.76 and 1.86?kg?C?ha?1?day?1, respectively, and those of daily N2O fluxes were 2.23 and 124?g?N?ha?1?day?1, respectively. The simulated gross CH4 emissions for CF and AWD from the puddling stage (2 days before transplanting) to harvest (97 days after transplanting) were 417 and 126?kg?C?ha?1, respectively; these values were 9.8% lower and 0.76% higher, respectively, than the observed values. The simulated gross N2O emissions during the same period were 0.0279 and 1.45?kg?N?ha?1 for CF and AWD, respectively; these values were respectively 87% and 29% lower than the observed values. The observed total global warming potential (GWP) of AWD resulting from the CH4 and N2O emissions was approximately one-third of that in the CF treatment. The simulated GWPs of both CF and AWD were close to the observed values despite the discrepancy in N2O emissions, because N2O emissions contributed much less than CH4 emissions to the total GWP. These results suggest that the DNDC-Rice model can be used to estimate CH4 emission and total GWP from tropical paddy fields under both CF and AWD conditions. 相似文献
36.
Tanaka A Yamane Y Matsuda H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2001,63(7):811-813
Although mast cells contribute to host protective immunity against bacterial infections, the exact mechanism of their recruitment at the affected site has been unclear. Recently, we have reported that both mouse and human mast cells are capable of producing matrix metalloproteinase (MMP)-9, a matrix-degrading enzyme necessary for leukocyte transmigration. Here, we demonstrated that bacterial lipopolysaccharide (LPS) enhanced MMP-9 production of mouse bone marrow derived-cultured mast cells. This action of LPS was partially suppressed by the pretreatment of cultured mast cells with a protein kinase C (PKC) inhibitor, indicating the possible involvement of PKC signaling pathways in the production of MMP-9 by LPS. Thus, these suggest the upregulation of mast cell MMP-9 by bacterial components, thereby resulting in their migration at the affected site. 相似文献
37.
The present studies were undertaken to characterise oxidative metabolism with diverse substrates in hepatic mitochondria of acidotic chicks. Metabolic acidosis was experimentally induced by replacement of drinking water with ammonium chloride solution (15 g/l) for 5 d. State 3 oxidation rates in liver mitochondria were significantly reduced in acidotic chicks only for pyruvate and glutamate as substrates requiring complex I, III and IV of the electron transport chain, while they were not changed for either succinate-requiring complexes II, III and IV, ascorbate+TMPD-requiring complex IV, or alpha-ketoglutarate requiring complexes I, III and IV. It can be concluded that the impairment of oxidation rate was substrate-specific in liver mitochondria of acidotic animals and not associated with functional damage of the respiratory chain in mitochondria. Possible reasons for the reductions in oxidation rate with pyruvate and glutamate are discussed. 相似文献
38.
Tanaka R Murota A Nagashima Y Yamane Y 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2002,16(4):446-451
Platelet life span was measured in dogs with experimentally-induced mitral regurgitation (MR) by using an in vitro whole-blood biotinylation technique to evaluate the effects of shear stress induced by MR. Mitral regurgitation was created in healthy dogs by ablation of the mitral valve chordae tendineae. Mitral regurgitation was identified by auscultation and postoperative color Doppler echocardiography. Platelets were biotinylated. and derivatized blood was reinfused into the dogs. Biotinylated platelet disappearance was measured over time to determine platelet life span. Pre- and postablation platelet life span was compared in each dog. Platelet life span was shorter in dogs with experimentally induced MR. Shear stress from MR was postulated to shorten platelet life span. Alternations in platelet function and life span may contribute to the progression of cardiovascular disease in dogs with MR. Further studies are required to determine if platelet dysfunction is related to disease progression in these patients. 相似文献
39.
40.
Prevalence of Bartonella henselae, Bartonella clarridgeiae and the 16S rRNA gene types of Bartonella henselae among pet cats in Japan 总被引:5,自引:0,他引:5
Maruyama S Nakamura Y Kabeya H Tanaka S Sakai T Katsube Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2000,62(3):273-279
The authors investigated bacteriologically the prevalence of Bartonella infection among 690 pet cats derived from 10 private animal hospitals in six cities (Sapporo, Hokkaido Prefecture; Sendai, Miyagi Prefecture; Joetsu, Niigata Prefecture; Fujisawa, Kanagawa Prefecutre; Kyoto, Kyoto Prefecture; Sanda, Hyogo Prefecutre) and 4 counties (Mishima, Osaka Prefecture; Hikawa, Shimane Prefecture; Aira, Kagoshima Prefecture; Shimajiri, Okinawa Prefecture) located from the north to the south of Japan. Bartonella species were isolated from 7.2% (50/690) of all the cats examined. No Bartonella species were isolated from the cats in Sapporo or Sendai. The isolation rate varied from 2% in Joetsu and Sanda to 20% in Shimajiri. Bartonella clarridgeiae was isolated from two of 50 cats in Kyoto, three of 50 in Mishima and one of 50 in Shimajiri, but not in cats from the other cities or counties. Though the cats of Joetsu, Fujisawa, Kyoto, Sanda, Aira and Shimajiri were infected with either B. henselae or B. clarridgeiae, one of eight infected cats in Mishima was harboring both Bartonella species. Type I of 16S rRNA gene was the predominant type among the isolates of B. henselae, but only one isolate derived from Shimajiri was found to be of type II. Prevalence of B. clarridgeiae and the 16S rRNA gene type of B. henselae among cats in Japan was demonstrated for the first time in this investigation. 相似文献