黄土丘陵区不同土地利用方式下土壤养分及生态化学计量特征

[目的]探究黄土丘陵区土地利用方式变化前后土壤养分及生态化学计量比的变化特征,为该区农业生产以及生态恢复工作提供科学依据。[方法]选取山西省永和县岔口小流域因生态工程的实施而改变土地利用方式的坡改梯地(梯田)、退耕还林地(林地)、地埂核桃园地(园地)3种样地为研究对象,以现有坡耕地为对照,利用时空互代法,比较不同土地利用方式变化前后0—20 cm,20—40 cm土壤C,N,P,K含量以及生态化学计量比的差异。[结果]①流域各种生态恢复措施实施后,土壤C,N元素含量明显增加,梯田、林地、园地0—40 cm土壤有机C含量分别是坡耕地的1.38,2.44和1.09倍,全N含量分别是坡耕地的1.76,2.66,1.68倍。P,K元素增加量不明显,林地相比其他2种土地利用方式对C,N元素的固持更加明显;②通过分析土壤养分化学计量比的关系,发现流域土壤的限制性元素为C,N元素;③流域土壤养分及计量比之间存在着稳定的耦合关系,C-N之间的耦合关系最为显著且在各元素和生态化学计量比稳定方面发挥着重要作用。[结论]流域3种土地利用方式有效地改变了土壤全量养分含量和元素的生态化学计量比,有利于流域的生态保护和植被修复。     

不同药剂组合对马铃薯晚疫病的防治效果

马铃薯晚疫病是马铃薯生产中的重要病害,是影响马铃薯产量和品质的重要因素之一,在云南春作马铃薯上普遍发生,且危害较重,给当地马铃薯产业带来了巨大的经济损失。为减轻马铃薯晚疫病对马铃薯生产造成的损失,试验研究了11种药剂7种药剂组合(包衣剂+保护剂+治疗剂)通过播种期种薯包衣,现蕾期叶面喷施一次保护剂,发病初期和发病中期叶面各喷一次治疗剂的方法防治马铃薯晚疫病。结果表明,药剂组合种薯包衣+甲霜·锰锌+氟吡菌胺·霜霉威的防治效果最好,防治效果达到60.99%,增产率176.28%,增效率152.27%;其次是种薯包衣+霜脲·锰锌+氟吡菌胺·霜霉威组合,防治效果达到60.15%,增产率173.20%,增效率150.55%。这2个药剂组合对马铃薯晚疫病均有极显著的防治效果,增产增效明显。     

Suppression subtractive hybridization and microarray analysis reveal differentially expressed genes in the <Emphasis Type="Italic">Lr39/41</Emphasis>-mediated wheat resistance to <Emphasis Type="Italic">Puccinia triticina</Emphasis>

Wheat leaf rust caused by Puccinia triticina (Pt) is one of the most severe fungal diseases threatening the global wheat production. The use of leaf rust resistance (Lr) genes in wheat breeding programs is the major solution to solve this issue. Wheat isogenic line carrying the Lr39/41 gene has shown a moderate to high resistance to most of the Pt pathotypes detected in China. In the present study, a typical hypersensitive response (HR) was observed using microscopy in leaves of the Lr39/41 isogenic line inoculated with the avirulent Pt pathotype THTT from 48 h-post inoculation. Two Lr39/41 resistance-associated suppression subtractive hybridization (SSH) libraries with a total of 6000 clones were established. Microarray hybridizations were performed on all obtained SSH clones using RNAs extracted from leaves of the Pt-inoculated and non-inoculated Lr39/41 isogenic lines, and leaves of the Pt-inoculated and non-inoculated Thatcher susceptible lines. Differentially expressed clones were analyzed by significance analysis of microarrays (SAM), followed by further sequencing. A total of 36 Lr39/41-resistance-related differentially expressed genes (DEGs) were identified, many of which had been previously reported to be involved in the plant defense response. The expression levels of eight selected DEGs during different stages of the Lr39/41-mediated resistance were further quantified by a qRT-PCR assay. Several pathogenesis-related (PR) and HR-related genes seem to be crucial for the Lr39/41-mediated resistance. In general, a brief profile of DEGs associated with the Lr39/41-mediated wheat resistance to Pt was drafted.     

Comparative study on calretinin immunoreactivity in gerbil and rat retina

Expression of calretinin in retina has been ascribed to multiple biological and functional aspects in the visual system. In this study, we examined the distribution patterns of calretinin immunoreactivity in gerbil and rat retina. In the gerbil, calretinin immunoreactivity was present in bipolar and amacrine cells of the inner nuclear layer and in neurones of the ganglion cell layer. In the rat, amacrine and ganglion cells showed calretinin immunoreactivity, but bipolar cells did not contain calretinin immunoreactivity. In both species, calretinin immunoreactivity was absent in cones, cone bipolars, and horizontal cells. In conclusion, gerbil as well as rat has a rod-dominant retina. The differences in calretinin expression between rat and gerbil require further investigations under various functional and developmental conditions.     

转盘式对虾开背工艺分析与参数优化

针对目前对虾开背环节工艺不完善、实际应用装备缺乏等问题,以中型南美白对虾为研究对象,探究了对虾开背关键工艺方法,建立了对虾开背过程力学模型,获得影响开背效果主要因素为刀具角度、加工中心盘转速和刀具安置距离;采用单因素试验研究和分析了各因素对开背成功率、虾仁损伤率、虾线裸露成功率和感官评分值的影响,确定影响因素最佳区间为：刀具角度20°~60°、加工中心盘转速10~40r/min和刀具安置距离9~11mm;利用Design-Expert软件进行多因素响应面试验研究分析,并采用多目标优化方法,得到最优参数组合为：刀具角度43.838°、加工中心盘转速28.391r/min、刀具安置距离9.801mm时,开背成功率为99.161%、虾仁损伤率为2.825%、虾线裸露成功率为90.727%、感官评分值为86.944。验证试验结果表明：针对中型南美白对虾,当刀具角度45°、加工中心盘转速28r/min、刀具安置距离9.8mm时,开背成功率为98.89%、虾仁损伤率为3.33%、虾线裸露成功率为87.78%、感官评分值为85.33,与理论优化值的绝对误差均较小,优化后的对虾开背装置性能满足作业要求。研究可为对虾开背工序装置设计和参数选择提供理论基础和科学依据。     

金属离子对糖化酶舌活性的影响

研究了六种金属离子对糖化酶活性的影响,结果表明不同的金属离子对糖化酶活性有不同程 度的影响,除Ｍｇ２＋有激活作用外,其他五种离子都有抑制作用。此外,还采用通用旋转组合回归设计方 法,探讨了Ｃｕ(２＋),Ｚｎ(２＋),Ｍｎ(２＋)抑制糖化酶活性的交互作用。     

油松花粉离体萌发及萌发特征研究

为了加强油松有性杂交,为种子园育种研究奠定基础,对油松花粉离体萌发的适宜条件及萌发特征进行了系统研究。结果表明,油松花粉在无蔗糖培养基中仍具一定萌发能力,蔗糖的添加可显著促进花粉萌发,但高浓度蔗糖可抑制花粉管的生长;油松花粉管易形成分枝,蔗糖浓度与分枝花粉频率呈正相关;硼酸的添加在培养初期可有效加速花粉萌发,但过高浓度的硼酸对花粉萌发具抑制作用;Ca2+对花粉萌发的影响较小,不同浓度Ca2+处理均可获得较高的萌发率;培养温度对花粉萌发的促进作用明显,但温度的持续升高将抑制花粉萌发。综合萌发率、花粉管生长及分枝花粉管频率,油松花粉萌发的最适液体培养基组成为：2.00%蔗糖+0.075%硼酸+0.005% Ca2+,26℃下光照培养,72 h时萌发率可达(96.10±0.47)%。     

Identification of QTLs associated with salinity tolerance at late growth stage in barley

Salinity is a major abiotic stress to barley (Hordum vulgare L.) growth and yield. In the current study, quantitative trait loci (QTL) for yield and physiological components at the late growth stage under salt stress and non-stress environments were determined in barley using a double haploid population derived from a cross between CM72 (salt-tolerant) and Gairdner (salt-sensitive). A total of 30 QTLs for 10 traits, including tiller numbers (TN), plant height, spikes per line (SPL), spikes per plant (SPP), dry weight per plant, grains per plant, grain yield, shoot Na⁺ (NA) and K⁺ concentraitions (K) in shoot, and Na⁺/K⁺ ratio (NAK), were detected, with 17 and 13 QTLs under non-stress and salt stress, respectively. The phenotypic variation explained by individual QTL ranged from 3.25 to 29.81%. QTL flanked by markers bPb-1278 and bPb-8437 on chromosomes 4H was associated with TN, SPL, and SPP under salt stress. This locus may be useful in the breeding program of marker-assisted selection for improving salt tolerance of barley. However, QTLs associated with NA, K, and NAK differed greatly between non-stress and salt stress environments. It may be suggested that only the QTLs detected under salt stress are really associated with salt tolerance in barley. D. Xue and Y. Huang contributed equally to the article.     

Metabolic characterization of newly isolated Pseudomonas nitroreducens Jin1 growing on eugenol and isoeugenol

Newly isolated soil bacterium strain Jin1 was able to grow on both eugenol and isoeugenol each as sole source of carbon and energy. Based on bacterial 16S rDNA analysis, Jin1 belongs to Pseudomonas nitroreducens with a similarity of 98.92% (14/1297). P. nitroreducens Jin1 was found to biotransform eugenol and isoeugenol to vanillin by different pathways. Eugenol was biotransformed to vanillin through coniferyl alcohol and ferulic acid similarly to the pathway shown previously by Pseudomonassp. HR199 and vanillin produced from eugenol was rapidly metabolized to vanillic acid. Contrastively, Pseudomonas nitroreducens Jin1 did not appear to produce metabolic intermediates during the biotransformation of isoeugenol to vanillin which was finally biotransformed to vanillic acid with much slower rate. These results indicate that there seems to be different metabolic regulation systems for the biotransformation of eugenol and isoeugenol by this bacterium. Herein, we report on Pseudomonas nitroreducens Jin1, a novel bacterium that produces vanillin from eugenol and isoeugenol by two different metabolic pathways.     

Molecular epidemiology of Newcastle disease viruses in Vietnam

Newcastle disease virus (NDV) causes significant economic losses to the poultry industry in Southeast Asia. In the present study, 12 field isolates of NDV were recovered from dead village chickens in Vietnam between 2007 and 2012, and were characterized. All the field isolates were classified as velogenic. Based on the sequence analysis of the F variable region, two distinct genetic groups (Vietnam genetic groups G1 and G2) were recognized. Phylogenetic analysis revealed that all the 12 field isolates fell into the class II genotype VII cluster. Ten of the field isolates, classified as Vietnam genetic group G1, were closely related to VIIh viruses that had been isolated from Indonesia, Malaysia, and Cambodia since the mid-2000s, while the other two field isolates, of Vietnam genetic group G2, clustered with VIId viruses, which were predominantly circulating in China and Far East Asia. Our results indicate that genotype VII viruses, especially VIIh viruses, are predominantly responsible for the recent epizootic of the disease in Vietnam.