从油橄榄引种看我国木本食用油料产业的发展

为了科学制定木本油料产业发展规划,论述了我国发展木本食用油料产业的优势和特点,分析了橄榄油的营养价值和市场动力,简述了我国油橄榄引种栽培的历史,指出了我国目前木本食用油料产业发展中存在的主要问题,并提出了促进木本食用油料产业发展的对策与措施。     

巴西养殖业和饲料工业发展现状

近几年,巴西的家禽和猪生产发展迅速,特别是自2002年起,无论是在量上还是在总额上,开始成为全球主要禽肉、猪肉出口国。这些都得益于其大豆、玉米等原料的自给自足。而巴西日益增长的玉米出口也给效率日益提高的畜禽生产增加了压力。巴西的主要玉米种植户也是联营企业主,他们在畜禽和饲料上进行投资,作为使其玉米“增值”的手段。同时,他们也发现,把目标瞄准那些有利可图的禽肉和猪肉出口市场会带来更多益处。纵向和横向的一体化发展势头在较大的玉米种植州巴拉那州较为迅猛——凭其散装产品和集装箱港的得天独厚的条件而优势突出。1巴拉那…     

矢量数据和栅格数据在土地预测中的应用对比——以山西岔口小流域为例

研究目的:为了对比矢量数据和栅格数据在土里利用过程中的差别。研究方法:借助于Super Map2008、MATLAB R2009a、Arc GIS9.2和IDRISI Andes15.0等软件,建立马尔科夫模型,得到转移概率矩阵进行对比分析。研究结果:矢量数据的结果一般更为精确,但软件利用程度低。栅格数据的结果比较而言没有那么精确,但是思路简单,软件利用程度高,结果易得且也能满足预测要求。研究结论:不论是矢量数据还是栅格数据都可以很好地用于土地利用中的预测。     

ELISA法对禽蛋中氯霉素残留量检测

试样中残留的氯霉素与氯霉素酶标记物共同竞争氯霉素抗体,形成有酶标记或无酶标记的抗原抗体复合物而被吸附于微孔底板。用酶标仪在波长450 nm处测定吸光度,根据吸光度得出样品中氯霉素的残留量。对禽蛋中氯霉素残留量进行测定,并对其加标回收率、再现性、重复性进行了分析研究,均能够达到国际氯霉素残留量的检测要求。     

浅析物联网与智慧林业

物联网是物物相连的互联网,其用途十分广泛,在传统林业向智慧林业的转变过程中起到了不可替代的作用。本文拟对物联网在林业中的资源监测、保护等方面的应用进行分析,以期为物联网在今后智慧林业方面的发展提供技术支持。     

细胞色素C氧化酶的纯化及动力学性质研究

采用硫酸铵分级沉淀和CytochromeC -Sepharose 4B亲和层析 ,从牛心线粒体中纯化了细胞色素C氧化酶 (CytochromeCOxidase ,简称CCO ) ,其比活性为 1 7 88s- 1 ·mg- 1 ,聚丙烯酰胺凝胶电泳为一条带 ,血红素A (HemeaA)含量达到 1 5 4nmol/mg。纯化后的CCO具有典型的CCO特征 ,还原态CCO峰值出现在 60 5nm和 443nm ,氧化态CCO峰值出现在 598nm和 42 4nm。CCO的稳态动力学参数Vm1、Km1、Vm2 、Km2 分别为 1 42s- 1 、 0 53μmol/L、 2 71s- 1 、 1 81 μmol/L。     

Effects of isorhapontigenin on cell cycle arrest and down-regulation of cyclin D1 expression in bladder cancer cells

AIM：To explore the inhibitory mechanism of isorhapontigenin (ISO) on the proliferation, migration and invasion of UMUC3 bladder cancer cells. METHODS：Human UMUC3 bladder cancer cells were pretreated with ISO, and the proliferation of the cells was observed under phase-contrast microscope and by ATPase assay. The expression of cyclin D1 was determined by RT-PCR and Western blotting. The cell cycle alteration was detected by flow cytometry, and the cell migration was examined by wound-healing assay. RESULTS：Over 20 μmol/L of ISO significantly inhibited the proliferation of UMUC3 cells with the IC50 of (22.5±2.8) μmol/L. The mRNA and protein levels of cyclin D1 in UMUC3 cells were markedly decreased after treatment with ISO. Exposure of UMUC3 cells to low dose (5 μmol/L) of ISO led to significant induction of G0/G1 growth arrest at both 12 h (58.82%) and 24 h (63.94%), compared with the negative control cells (47.33%) without inducing obvious apoptosis. ISO at dose of 5 μmol/L also markedly inhibited the cell migration. CONCLUSION：ISO significantly exhibits inhibitory effects on the proliferation and migration of human bladder cancer cells by down-regulation of cyclin D1 expression accompanying with G0/G1 cell cycle arrest.     

Immune responses in piglets infected with highly pathogenic porcine reproductive and respiratory syndrome virus

Porcine reproductive and respiratory syndrome virus (PRRSV) infection compromises the host's innate and adaptive immunity. The aim of this study was to investigate the immune responses of piglets infected with highly pathogenic (HP) PRRSV (HuN4 strain) with or without the immunization with CH-1R attenuated PRRSV vaccine. The response was evaluated for the clinical signs, pathological changes and virus load in immune organs, antibody responses and levels of serum IFN-γ, IL-4 and IL-10. The result showed that in comparison with the piglets received the immunization, the piglets infected with HP-PRRSV alone had the thymus atrophy, decreased serum levels of IL-4 and increased serum levels of IL-10 and INF-γ. These results suggest that elevated IL-10 levels at the early stage of the infection may enhance virus survival and delay the induction of protective immunity, while increased levels of IL-4 induce the effective immune responses and increase the animals' health status.