奶水牛乳中体细胞数与乳成分的相关分析

乳汁体细胞数(somatic cell count,SCC)是检查奶牛乳房炎和牛奶质量的指标,为了解南宁某规模化奶牛场奶水牛SCC情况及SCC与其他乳成分的关系,试验随机选取该场152份泌乳早期乳样,测定其SCC、乳汁比重、蛋白质、脂肪、总固形物、非脂固形物及乳糖的含量,并对其进行相关性分析。结果表明,SCC小于50万/mL的乳样占被检奶样总数的88.16%,大于100万/mL的占1.97%;乳汁比重、乳蛋白、乳糖和非脂固形物含量均随着SCC的升高而下降,而乳脂含量随着SCC的升高而呈上升趋势;SCC与乳糖及非脂固形物含量呈极显著负相关(P<0.01)。因此,奶水牛乳中SCC不仅与乳糖和非脂固形物含量密切相关,也提示其与奶水牛产后营养代谢状况有关。     

计算机串口与GSM手机通讯的研究与实现

文章对基于计算机串口的GSM短信通讯系统进行研究,实现在计算机上通过串口连接GSM模块并使用GSM网络与远端的手机进行通讯。设计中采用C#程序开发语言和运用计算机串口通讯技术,实现计算机通过串口对GSM模块（TC35）进行控制,并解决了PDU编码解码、AT指令控制TC35、以及软件界面的设计等问题,最终完成计算机终端实时与TC35模块通讯,实现短信息服务。研究表明,通过拓展计算机串口与GSM模块的应用可以建立各种远程控制,达到较好的远程控制效果。     

Low levels of haptoglobin and putative amino acid sequence in Taiwanese Lanyu miniature pigs

Porcine haptoglobin (Hp) is an acute phase protein. Its plasma level increases significantly during inflammation and infection. One of the main functions of Hp is to bind free hemoglobin (Hb) and inhibit its oxidative activity. In the present report, we studied the Hp phenotype of Taiwanese Lanyu miniature pigs (TLY minipigs; n=43) and found their Hp structure to be a homodimer (beta-alpha-alpha-beta) similar to human Hp 1-1. Interestingly, Western blot and high performance liquid chromatographic (HPLC) analysis showed that 25% of the TLY minipigs possessed low or no plasma Hp level (<0.05 mg/ml). The Hp cDNA of these TLY minipigs was then cloned, and the translated amino acid sequence was analyzed. No sequences were found to be deficient; they showed a 99.7% identity with domestic pigs (NP_999165). The mean overall Hp level of the TLY minipigs (0.21 +/- 0.25 mg/ml; n=43) determined by enzyme-linked immunosorbent assay (ELISA) was markedly lower than that of domestic pigs (0.78 +/- 0.45 mg/ml; p<0.001), while 25% of the TLY minipigs had an Hp level that was extremely low (<0.05 mg/ml). In addition, the initial recovery rate (first 40 min) in the circulation of infused fluorescein isothiocyanate (FITC)-Hb was significantly higher in the TLY minipigs with extremely low Hp levels than those with high levels. This data suggests that the low concentration of Hp-Hb complex is responsible for the higher recovery rate of Hb in the circulation. TLY minipigs have been used as an experimental model for cardiovascular diseases; whether they can be used as a model for inflammatory diseases, with Hp as a marker, remains a topic of interest. However, since the Hp level varies significantly among individual TLY minipigs, it is necessary to prescreen the Hp levels of the animals to minimize variation in the experimental baseline. The present study may provide a reference value for future use of the TLY minipig as an animal model for inflammation-associated diseases.     

我国大豆精量播种机械发展现状及趋势

大豆播种机械作为大豆机械化生产中的主要工具,其性能的优劣直接影响到播种质量及大豆产量。介绍了我国东北地区、黄淮海区域和南方区域大豆精密播种技术的应用现状,阐述了大豆精量排种器以及免耕防堵技术的理论研究现状,指出我国大豆精密播种技术需要解决的关键问题,从而为我国大豆精量播种机械的发展方向提供参考。     

基于CI-600的绿萝根系生长状态监测

【目的】探究绿萝生长与水分胁迫之间的关系,为绿萝种养提供合适的灌溉参考依据,减少农业生产水资源浪费问题。【方法】选取绿萝为试验材料,对其进行4组不同梯度的水分处理,通过CI-600植物根系生长监测设备采集并分析根数量、根长度、根面积、根体积与水分胁迫之间的关系,并利用Excel软件进行数据处理,绘制相关参数与土壤水分胁迫之间的关系曲线,采用最小二乘法,运用二次项拟合的方法进行模型拟合,最终得出植物根系生长与水分胁迫之间的关系。【结果】利用二次项进行拟合的模型,决定系数R2绝大多数大于0.9,拟合程度高。在35%湿度条件下绿萝根数量、根面积、根体积增长速率最快,在25%～35%湿度区间内有利于根长度的增加。从拟合曲线看,25%、35%湿度条件下根数量及根长度两个指标较为一致。水分胁迫通过影响根长度及根数量,进而作用于根系面积和体积的变化;根系伸长也依赖于其直径的拓展以获取有效的吸收面积;根系体积的变化与根数量、根系直径等均有联系。【结论】绿萝最适宜的生长湿度为35%,干旱胁迫（15%）和淹水胁迫（45%）对根系生长均具有不利的影响;根系的各项生理指标在不同的水分胁迫中有不同的趋势和表现,...     

北京市马属动物赛事监管制度的建立

通过对近年来重大马术活动的监管,结合《北京市动物防疫条例》的实施,北京市逐步形成马属动物展览演出比赛的“赛事报告、报检报验、档案记录、防疫条件审查、隔离观察、赛中监管、应急预备和宣传告知”八大监管制度,确保了北京市马属动物无疫安全。     

赖氨酸采食量对早期断奶仔猪生长发育及胰腺内分泌机能的影响

将55头21日龄断奶的雄性仔猪随机分为5组，各组仔猪饲喂赖氨酸含量为0．60％、0．80％、1．00％、1．20％和1．40％的试验日粮21d。42日龄结束饲养试验时进行葡萄糖灌注试验，观察赖氨酸采食量对早期断奶仔猪生长发育和胰腺内分泌的影响。结果表明：早期断奶仔猪体增重与赖氨酸采食量为强正相关，根椐体增重指标，21～42日龄阶段，仔猪的赖氨酸采食量不应低于3．16g／d，日粮赖氨酸含量不应低于1.40％。赖氨酸严重缺乏(采食量小于1．45g／d)时，胰岛素的分泌量下降，对高血糖应激的反应能力降低。     

稻谷颗粒模型离散元接触参数标定与试验

EDEM与FLUENT耦合模拟稻谷清选时,稻谷颗粒参数设置的准确性直接影响仿真结果的可信度。结合稻谷颗粒堆积角的实测试验与仿真试验,标定了稻谷颗粒模型间静摩擦系数、滚动摩擦系数2个主要接触参数。设计了堆积角形成装置,该装置采用坍塌法和注入法同时形成2种堆积角,可以减小非同时形成所引起的测量误差;创建了稻谷颗粒的离散元模型,结合堆积角的实测试验与仿真试验,建立了2个主要接触参数与2种堆积角之间的二元回归方程;以稻谷颗粒2种堆积角的实测结果作为目标值,对回归方程进行数值求解,得到颗粒模型间的静摩擦系数、滚动摩擦系数分别为0.433 6、0.161 5;设计了抛洒试验对标定后的参数进行验证,抛洒长度、宽度和近抛洒端堆积角等特征参数的实测结果与仿真结果的相对误差小于5.7%,这表明颗粒的模拟运动轨迹与实际运动轨迹基本一致,所创建的稻谷颗粒离散元模型及标定得到的接触参数可以为EDEM-FLUENT耦合仿真稻谷清选过程提供参考。     

Injection molding of vertebral fixed cage implant

A vertebral cage is a hollow medical device which is used in spine surgery. By implanting the cage into the spine column, it is possible to restore disc and relieve pressure on the nerve roots. Most cages have been made of titanium alloys but they detract the biocompatibility. Currently PEEK (polyether ether ketone) is applied to various implants because it has good properties like heat resistance, chemical resistance, strength, and especially biocompatibility. A new shape of vertebral cage is designed and injection molding of PEEK is considered for production. Before injection molding of the cage, it is needed to evaluate process conditions and properties of the final product. Variables affecting the shrinkage of the cage are considered, e.g., injection time, packing pressure, mold temperature, and melt temperature. By using the numerical simulation program, MOLDFLOW, several cases are studied. Data files obtained by MOLDFLOW analysis are used for stress analysis with ABAQUS, and shrinkage and residual stress fields are predicted. With these results, optimum process conditions are determined.     

马白细胞介素18成熟蛋白(mEIL-18)基因在大肠埃希氏菌中的高效表达与纯化

用RT—PCR从经ConA刺激的马外周血单个核细胞（PBMC）中扩增出马白细胞介素18（Equine interleukin-18，EIL-18）前体蛋白（precursor EIL-18，pEIL-18）基因的cDNA，然后克隆至载体pCR2．1-TOPO中，鉴定并命名为pCR2．1-pEIL-18。自pCR2．1-pEIL-18中扩增马白细胞介素18成熟蛋白（mature EIL-18，mEIL-18）基因，并将其亚克隆至原核表达载体pET-28a（＋）中。将筛选出的阳性克隆进行测序、诱导表达并纯化其表达产物。结果表明，mEIL-18基因全长474bp，含1个开放阅读框，编码157个氨基酸的成熟蛋白；表达产物以可溶性和包涵体两种形式存在，经SDS-PAGE和Western—blot分析，重组蛋白相对分子量约为20ku，且具有免疫生物学活性。mEIL-18基因在大肠杆菌中高效表达并在非变性和变性条件下采用Ni^＋亲和层析纯化方法获得了高纯度的重组mEIL-18，为探究马白细胞介素18的生物学活性及其应用奠定了基础。