全文获取类型
收费全文 | 1030篇 |
免费 | 54篇 |
专业分类
林业 | 29篇 |
农学 | 44篇 |
基础科学 | 3篇 |
102篇 | |
综合类 | 260篇 |
农作物 | 124篇 |
水产渔业 | 87篇 |
畜牧兽医 | 382篇 |
园艺 | 11篇 |
植物保护 | 42篇 |
出版年
2023年 | 3篇 |
2022年 | 15篇 |
2021年 | 31篇 |
2020年 | 8篇 |
2019年 | 15篇 |
2018年 | 20篇 |
2017年 | 14篇 |
2016年 | 24篇 |
2015年 | 18篇 |
2014年 | 27篇 |
2013年 | 47篇 |
2012年 | 68篇 |
2011年 | 74篇 |
2010年 | 40篇 |
2009年 | 40篇 |
2008年 | 83篇 |
2007年 | 85篇 |
2006年 | 85篇 |
2005年 | 70篇 |
2004年 | 49篇 |
2003年 | 46篇 |
2002年 | 48篇 |
2001年 | 38篇 |
2000年 | 26篇 |
1999年 | 13篇 |
1998年 | 4篇 |
1997年 | 6篇 |
1996年 | 3篇 |
1995年 | 2篇 |
1994年 | 2篇 |
1993年 | 2篇 |
1992年 | 4篇 |
1990年 | 6篇 |
1989年 | 2篇 |
1988年 | 4篇 |
1987年 | 4篇 |
1986年 | 6篇 |
1985年 | 8篇 |
1984年 | 5篇 |
1983年 | 6篇 |
1980年 | 3篇 |
1978年 | 4篇 |
1977年 | 2篇 |
1976年 | 2篇 |
1975年 | 3篇 |
1974年 | 3篇 |
1973年 | 4篇 |
1971年 | 2篇 |
1970年 | 3篇 |
1969年 | 2篇 |
排序方式: 共有1084条查询结果,搜索用时 15 毫秒
131.
Eun-Joo Kim Sang-Hee Jeong Joon-Hyoung Cho Hyun-Ok Ku Hyun-Mi Pyo Hwan-Goo Kang Kyoung-Ho Choi 《Journal of veterinary science (Suw?n-si, Korea)》2008,9(3):257-266
This study aimed to discover potential biomarkers for dioxynivalenol (DON) intoxication. B6C3F1 male mice were orally exposed to 0.83, 2.5 and 7.5 mg/kg body weight (bw) DON for 8 days and the differential protein expressions in their blood plasma were determined by SELDI - Time-of-Flight/Mass Spectrometry (TOF/MS) and the immunoglobulins (Igs) G, A, M and E in the serum were investigated. 11.7 kDa protein was significantly highly expressed according to DON administration and this protein was purified by employing a methyl ceramic HyperD F column with using optimization buffer for adsorption and desorption. The purified protein was identified as a haptoglobin precursor by peptide mapping with using LC/Q-TOF/MS and MALDI-TOF/MS and this was confirmed by western blotting and ELISA. IgG and IgM in serum were decreased in a dose-dependent manner and IgA was decreased at 7.5 mg/kg bw DON administration, but the IgE level was not changed. To compare the expressions of haptoglobin and the Igs patterns between aflatoxin B1 (AFB1), zearalenone (ZEA) and DON intoxications, rats were orally administered with AFB1 1.0, ZEA 240 and DON 7.5 mg/kg bw for 8 days. Haptoglobin was increased only at DON 7.5 mg/kg bw, while it was slightly decreased at ZEA 240 mg/kg bw and it was not detected at all at AFB1 1.0 mg/kg bw. IgG and IgA were decreased by DON, but IgG, IgA, IgM and IgE were all increased by AFB1. No changes were observed by ZEA administration. These results show that plasma haptoglobin could be a diagnostic biomarker for DON intoxication when this is combined with examining the serum Igs. 相似文献
132.
Although the widespread occurrence of calf diarrhoea (CD) bovine coronavirus (BCoV) infections have been reported in most cattle producing countries, only the genetic differences in the BCoVs from American and Canadian isolates and/or strains have been identified and compared. Hence, it is unclear if the BCoVs circulating in the other countries have distinct genetic characteristics. The aim of this study was to determine the prevalence and genetic diversity of CD BCoVs based on the deduced amino acid (aa) sequences of the spike (S) and haemagglutinin/esterase (HE) proteins in South Korea. RT-PCR and nested PCR using the primer pairs specific to the nucleocapsid gene, BCoVs detected the BCoVs in 56 (15.6%) of 359 diarrhoeic faecal samples. Phylogenetic analysis of the entire S gene indicated that 10 Korean CD BCoV strains clustered with other Korean BCoV strains with different clinical forms but were different from the American and Canadian BCoV strains. Moreover, the phylogenetic data of the aa sequences of the HE gene revealed all the Korean CD strains to be distinct from the other Korean BCoV strains with different clinical forms. These results suggest that the Korean BCoVs cause endemic infections in diarrhoeic calves in Jeonnam province and have taken a different evolutionary pathway from the BCoVs in other countries. Moreover, the different BCoV strains are circulating in the different clinical forms in South Korea. These results also suggest that vaccines against the BCoVs can be developed with each Korean BCoV in different clinical forms. 相似文献
133.
The apparent digestibility of 20 rendered animal protein ingredients from various origins was determined in three digestibility trials. The ingredients consisted of eight blood meals, four feather meals, six meat and bone meals and two poultry by-product meals. Within each type of ingredient, a relatively large range of raw materials, processing conditions and equipment were represented. In each of three trials, a reference diet was mixed with test ingredients in a 70:30 ratio to produce a series of test diets. The reference and test diets were fed to rainbow trout reared at 15°C and fecal samples were collected using the “Guelph system”. Apparent digestibility coefficients (ADC) for protein and energy of the four feather meals varied between 81 and 87% and 76 and 80%. Significant differences in the ADC for protein of feather meal were observed and may be related to the drying equipment. The ADC for protein of the two poultry by-product meals was 87 and 91% and that of energy 77 and 87%. The ADC for protein and energy of the six meat and bone meals varied between 83 and 89% and 68 and 83%, respectively. Treatment of one of the meat and bone meals by air-classification to reduce its ash content resulted in a significant increase in the ADC for protein and lipid. ADC for protein of the blood meals varied between 82 and 99% whereas ADC for energy varied between 79 and 99%. Spray-dried blood products (whole blood, blood cells, blood plasma) were highly digestible (ADC protein=96-99%). Rotoplate-, steam-tube- and ring-dried blood meals had significantly lower ADC for protein and energy than spray-dried blood products. 相似文献
134.
Sung Hwoan Cho 《Journal of the World Aquaculture Society》2011,42(1):90-95
The effect on growth and body composition of various dietary additives with putative growth or health‐enhancing properties were determined in juvenile olive flounder (25 g initial weight). Nine experimental diets were prepared to contain one of the following additives: control (Con) with no additive, Opuntia ficus‐indica ver. saboten (OF), propolis (PP), lactic acid bacteria (LA), γ‐poly‐glutamic acid (PG), onion extract (OE), organic sulfur (OS), Biostone® (BS), and fig extract (FE). Fishmeal, dehulled soybean meal, and corn gluten were used as the protein source of the experimental diets. Wheat flour and soybean oil were used as the carbohydrate and lipid sources, respectively. Dietary additives were included in each experimental diet at 1% at the expense of wheat flour except for the FE (aqueous), which was substituted at 1% of the amount of water added to the diet. Fish were hand‐fed to satiation twice a day for 6 d/wk for 6 wk. Weight gain of fish fed the OE diet was higher than that of fish fed with the PP diet. Chemical composition of fish was not different among the experimental diets. OE was the most effective dietary additive to improve performance of olive flounder among additives used in this study. 相似文献
135.
Dong-Gyun Kim Yu-Ri Kim Eun-Young Kim Hyun Min Cho Sun-Hee Ahn In-Soo Kong 《Fisheries Science》2010,76(5):803-810
Vibrio anguillarum is a major pathogenic bacterium that causes vibriosis and septicemia in fish and shellfish. In this study, we identified the groESL genes, which encode bacterial chaperonins, from V. anguillarum. The groE gene cluster consisted of a 291-bp groES gene, a 69-bp intergenic spacer region, and a 1,635-bp groEL gene order. Sequence analysis with the groESL gene of Vibrio species exhibited that the groEL gene was more species-specific and suitable than the groES gene for V. anguillarum detection. Owing to the difficulty in distinguishing V. anguillarum from the closely related V. ordalii, we compared the sequences of groEL from V. anguillarum and the groEL homolog hsp60 from V. ordalii, in order to design a primer set based on a region dissimilar between the two. PCR with the groEL primer set produced a clear 195-bp amplicon in six serotypes of V. anguillarum, whereas 23 Vibrio species of 39 samples, including V. ordalii, and 10 species of enteric bacteria gave no bands. PCR using the groEL primers also amplified a unique product from V. anguillarum-infected flounder and oyster tissues. These results demonstrate that the groEL-target PCR assay is a sensitive and species-specific tool for the detection of V. anguillarum infection. 相似文献
136.
Wimonmuang Kanphassorn Cho Yoo-Hyun Lee Young-Sang 《Journal of Crop Science and Biotechnology》2023,26(1):97-106
Journal of Crop Science and Biotechnology - To understand high temperature-induced alterations in flavor-determining volatile property of fragrant rice, the brown rice of... 相似文献
137.
138.
Chi-Heung Cho Chang-Jun Lee Min-Gyeong Kim Bomi Ryu Jun-Geon Je Yoonsook Kim Sang-Hoon Lee 《Marine drugs》2022,20(6)
Advanced glycation end-products (AGEs) play a vital role in the pathogenesis of diabetic complications. Methylglyoxal (MGO), one of the major precursors of AGEs, is a highly reactive dicarbonyl compound that plays an important role in the pathogenesis of diabetic nephropathy. This study was designed to evaluate the therapeutic potential of phlorotannin-rich Ecklonia cava extract (ECE) on MGO-induced diabetic nephropathy in in vitro models using mouse glomerular mesangial cells. ECE showed anti-glycation activity via breaking of AGEs-collagen cross-links and inhibition of AGEs formation and AGE-collagen cross-linking formation. The renoprotective effects were determined by assessing intracellular reactive oxygen species (ROS) and MGO accumulation, cell apoptosis, and the Nrf-2/ARE signaling pathway. MGO-induced renal damage, intracellular ROS production level, and MGO-protein adduct accumulation were significantly decreased by pretreating ECE. Moreover, ECE pretreatment exhibited preventive properties against MGO-induced dicarbonyl stress via activation of the Nrf2/ARE signaling pathway and reduction of RAGE protein expression in mouse glomerular mesangial cells. Collectively, these results indicated potential anti-glycation properties and prominent preventive effects of ECE against MGO-induced renal damage. Additionally, ECE may be utilized for the management of AGE-related diabetic nephropathy. 相似文献
139.
An ELISA for porcine reproductive and respiratory syndrome: production of antigen of high quality. 总被引:4,自引:3,他引:4 下载免费PDF全文
A reliable method was developed to produce a viral antigen preparation from porcine reproductive and respiratory syndrome virus (PRRSV) infected MARC-145 cells by solubilizing the virus with Triton X-100. This method eliminated problems previously encountered with high background reactions associated with PRRSV antigen or cell control antigen. With this new antigen, an indirect enzyme-linked immunosorbent assay (ELISA) was adapted to detect swine serum anti-body against PRRSV. In the ELISA, non-specific reactions associated with test serum samples have been eliminated by utilizing an effective blocking serum diluent. The ELISA is more sensitive than an indirect immunofluorescent assay (IFA), particularly with late-infection sera, while maintaining a high diagnostic specificity. In a comparison of IFA and ELISA using sera collected from 250 pigs of various ages from 5 herds that had PRRS histories, IFA revealed 178 positive samples and 72 negative samples. All of the IFA-positive sera were proven to be ELISA reactors. However, nearly one-half (34/72) of the IFA-negative samples were also ELISA reactors. The diagnostic specificity and sensitivity of the ELISA were 100% and 96.6% with 257 serum samples collected from known healthy PRRS-negative swine herds and 57 sera collected from infected swine at 6 to 56 days after infection, respectively. The ELISA is technically superior to IFA, time-efficient and cost-effective, and suitable for testing of a large number of samples over a short period of time. 相似文献
140.
A-Tai Truong Sedat Sevin Seonmi Kim Mi-Sun Yoo Yun Sang Cho Byoungsu Yoon 《Journal of veterinary science (Suw?n-si, Korea)》2021,22(3)
BackgroundThe microsporidian parasite Nosema ceranae is a global problem in honeybee populations and is known to cause winter mortality. A sensitive and rapid tool for stable quantitative detection is necessary to establish further research related to the diagnosis, prevention, and treatment of this pathogen.ObjectivesThe present study aimed to develop a quantitative method that incorporates ultra-rapid real-time quantitative polymerase chain reaction (UR-qPCR) for the rapid enumeration of N. ceranae in infected bees.MethodsA procedure for UR-qPCR detection of N. ceranae was developed, and the advantages of molecular detection were evaluated in comparison with microscopic enumeration.ResultsUR-qPCR was more sensitive than microscopic enumeration for detecting two copies of N. ceranae DNA and 24 spores per bee. Meanwhile, the limit of detection by microscopy was 2.40 × 104 spores/bee, and the stable detection level was ≥ 2.40 × 105 spores/bee. The results of N. ceranae calculations from the infected honeybees and purified spores by UR-qPCR showed that the DNA copy number was approximately 8-fold higher than the spore count. Additionally, honeybees infected with N. ceranae with 2.74 × 104 copies of N. ceranae DNA were incapable of detection by microscopy. The results of quantitative analysis using UR-qPCR were accomplished within 20 min.ConclusionsUR-qPCR is expected to be the most rapid molecular method for Nosema detection and has been developed for diagnosing nosemosis at low levels of infection. 相似文献