全文获取类型
收费全文 | 1993篇 |
免费 | 102篇 |
专业分类
林业 | 51篇 |
农学 | 56篇 |
基础科学 | 3篇 |
336篇 | |
综合类 | 281篇 |
农作物 | 245篇 |
水产渔业 | 141篇 |
畜牧兽医 | 852篇 |
园艺 | 25篇 |
植物保护 | 105篇 |
出版年
2022年 | 15篇 |
2021年 | 37篇 |
2020年 | 22篇 |
2019年 | 26篇 |
2018年 | 49篇 |
2017年 | 46篇 |
2016年 | 47篇 |
2015年 | 45篇 |
2014年 | 60篇 |
2013年 | 91篇 |
2012年 | 103篇 |
2011年 | 136篇 |
2010年 | 64篇 |
2009年 | 57篇 |
2008年 | 96篇 |
2007年 | 108篇 |
2006年 | 68篇 |
2005年 | 79篇 |
2004年 | 71篇 |
2003年 | 75篇 |
2002年 | 51篇 |
2001年 | 33篇 |
2000年 | 43篇 |
1999年 | 37篇 |
1998年 | 11篇 |
1997年 | 20篇 |
1996年 | 16篇 |
1994年 | 14篇 |
1992年 | 23篇 |
1991年 | 33篇 |
1990年 | 31篇 |
1989年 | 27篇 |
1988年 | 20篇 |
1987年 | 28篇 |
1986年 | 27篇 |
1985年 | 27篇 |
1984年 | 20篇 |
1983年 | 19篇 |
1979年 | 20篇 |
1978年 | 11篇 |
1977年 | 19篇 |
1976年 | 11篇 |
1974年 | 17篇 |
1973年 | 23篇 |
1972年 | 18篇 |
1971年 | 14篇 |
1970年 | 18篇 |
1969年 | 19篇 |
1968年 | 12篇 |
1966年 | 11篇 |
排序方式: 共有2095条查询结果,搜索用时 63 毫秒
71.
Sprott LR Harris MD Forrest DW Young J Zhang HM Oyarzo JN Bellin ME Ax RL 《Journal of animal science》2000,78(4):795-798
In this study, semen samples from 25 bulls that had passed a breeding soundness evaluation were analyzed for the presence or absence of a 31-kDa protein, known as fertility-associated antigen (FAA), on spermatozoal membranes. Eighteen bulls had FAA on sperm (FAA-positive) and seven were devoid of FAA on sperm (FAA-negative). A single ejaculate from each bull was extended and frozen with 25 to 30 x 10(6) sperm in .5-mL straws. Crossbred replacement heifers (n = 865) were estrus-synchronized and artificially inseminated either at timed AI or 12 h after they were detected in estrus. Mature cows (n = 285) were inseminated 12 h after they were detected in estrus during a 45-d AI period. Pregnancy rates (pooled) to first AI service for females (n = 764) inseminated with FAA-positive sperm were 65.6% and were 49.7% for females (n = 386) inseminated with FAA-negative sperm (P < .005). Among the estrus-synchronized replacement heifers, pregnancy rates to synchronized AI service for heifers (n = 550) inseminated with FAA-positive sperm were 62% and were 45.7% for heifers (n = 315) inseminated with FAA-negative sperm (P < .005). These data indicate that pregnancy rates to first AI service at spontaneous and synchronized estrus are higher when using semen from bulls with detectable FAA on spermatozoal membranes compared to semen from bulls devoid of FAA on membranes. Fertility-associated antigen is an important determinant for fertility potential of sperm from bulls to be used in AI breeding programs. 相似文献
72.
Monoamines are important brain neurotransmitters. An investigation was carried out to determine if hypomagnesaemic tetany was associated with alterations in regional brain monoamine concentrations in bovines. The results, established in cows with normal magnesium status, demonstrated that regional differences existed in the distribution and concentration of brain monoamines in the adult bovine, which were similar to those in other species. In magnesium-deficient cows, severe hypomagnesaemia and lowered cerebrospinal fluid (CSF) magnesium concentrations were associated with significant alterations in monoamine concentrations in some brain regions. Alterations in 3,4-dihydroxyphenylalanine (DOPA) and dihydroxyphenylacetic acid (DOPAC) concentrations in the corpus striatum, and dopamine (DA) in the cerebral cortex and cerebellum were recorded. These regions play an important role in both voluntary and involuntary motor function, and therefore these alterations may play a role in the aetiology of hypomagnesaemic tetany. However, there was no significant change in DA concentrations in the corpus striatum (the main dopaminergic region in the brain) associated with hypomagnesaemia. In addition, a significantly lower norepinephrine (NE) concentration in the corpus striatum of hypomagnesaemic animals was also recorded. Norephinephrine is generally excitatory and therefore lowered NE concentrations would be expected to result in depression rather than stimulation of motor function. 相似文献
73.
FJ Pena JA Gines J Duque V Vieitez R Martinez –Pérez L Madejón I Nuñez Martinez JM Moran S Fernández-García 《Reproduction in domestic animals》2006,41(2):189-190
A 6-year-old female Alaska Malamute dog was presented for evaluation of abdominal enlargement referred by a local veterinarian. On the history, the owner complained of chronic abdominal enlargement initiated more than 4 months ago, reduced appetite, occasional vomiting and general dullness. He also complained of greenish mucous intermittent vaginal discharge starting 10 days ago. The bitch was chronically treated with medroxiprogesterone acetate. A laparatomy was performed and fluid in the abdomen was found and aspirated during the surgery. Also a very fluid-filled distended uterus and a mass in the distal part of the left uterine horn were found. The mass was encapsulated by the omentum, but areas of necrosis and calcification were identified. Histopathological diagnosis was endometrial adenocarcinoma. 相似文献
74.
Genetic characterization of canine rotavirus isolated from a puppy in Korea and experimental reproduction of disease. 总被引:3,自引:0,他引:3
75.
Marie Ren Gramer Jee Hoon Lee Young Ki Choi Sagar M. Goyal Han Soo Joo 《Canadian journal of veterinary research》2007,71(3):201-206
The H3N2 subtype of influenza A viruses isolated from pigs in the United States and Canada has shown both genetic and antigenic diversity. The objective of this study was to determine the serologic and genetic characteristics of contemporary strains of these viruses. Genetic analysis of 18 reference strains and 8 selected strains demonstrated differences in 1% to 9% of the nucleotides of the hemagglutinin (HA) gene. Phylogenetic analysis of the HA gene revealed 3 genetic clusters, as well as divergence of cluster III viruses from a cluster III prototype virus (A/Swine/Illinois/21587/99). By means of 1-way cross-hemagglutination inhibition with antiserum against 5 field isolates and 3 vaccine viruses, most of 97 isolates tested could be placed in 1 of 3 serogroups. The several isolates that did not react with any antiserum were in genetic cluster III, which suggests that continuous antigenic drift in cluster III may have resulted in virus variants. The efficacy of commercial vaccines against these virus variants should be evaluated with vaccination and challenge studies. 相似文献
76.
77.
78.
79.
Physiological Responses of Rainbow Trout Oncorhynchus mykiss to Crowding and Anesthesia with AQUI-S™
Glen W. Davidson Peter S. Davie Graham Young Reece T. Fowler 《Journal of the World Aquaculture Society》2000,31(1):105-114
Abstract.— Following exposure to the anesthetic AQUI-S™, plasma cortisol concentration in immature rainbow trout was measured as (mean) 293 ± 48 ng/ mL, which was significantly ( P > 0.05) higher than the mean concentration in resting fish. Cortisol concentrations remained significantly ( P > 0.05) elevated for at least 24 h after treatment. This was accompanied by a significant increase and decrease in hematocrit and plasma potassium, respectively. These perturbations continued for at least 48 h following recovery from anesthesia. Plasma concentrations of total protein and sodium remained unchanged following anesthesia with AQUI-S™. Crowding stress is commonly encountered by fish during manipulation in aquaculture situations. Anesthetising fish prior to, and during, manipulation may reduce the associated stress. Changes in cortisol values resulting from crowding (30 min; 0.1 kg/L) during anesthesia with AQUI-S™ were not appreciably different from those in fish crowded without anesthesia. Thus, anesthesia with AQUI-S™ at the recommended dose of 17 mg/L did not appear to be effective for alleviating the stress of crowding under the conditions of our experiments. 相似文献
80.
The pathogenesis of velogenic Newcastle disease virus infection of chickens of different ages and different levels of immunity 总被引:2,自引:0,他引:2
Chickens of 7 weeks or 20 weeks of age were divided into three groups according to their antibody status (high, low, absent) and were infected with a velogenic viscerotropic Newcastle disease virus. To follow patterns of viral replication, birds were necropsied at regular intervals up to 22 days and organs were sampled from each bird. In non-immune birds, virus could be isolated from all organs examined. In birds with antibody, virus was most frequently isolated from the proventriculus, cecal tonsil, bursa, and brain. However, because no one organ could be recommended for all situations, all four should be sampled for field diagnosis. In immune birds, although clinical signs were either mild or absent, widespread virus replication occurred up to 19 days post-challenge. 相似文献