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91.
92.
Akhil Ranjan Baruah Noriko Ishigo-Oka Mieko Adachi Yasuyo Oguma Yoshiro Tokizono Kazumitsu Onishi Yoshio Sano 《Euphytica》2009,165(3):459-470
The present study was conducted to understand the pattern of variation and the genetic bases for cold tolerance at the early
growth stage in Asian rice. The genetic variation was investigated at the germination, plumule and seedling stages among 57
strains including cultivated rice (Oryza sativa ssp. indica and ssp. japonica) and its wild progenitor (Oryza rufipogon). The significant differentiation of cold tolerance was observed among the taxonomically divided groups. At the germination
stage, both indica and japonica subspecies tended to be more tolerant than O. rufipogon, whereas at the plumule and seedling stages, ssp. japonica tended to be more tolerant than ssp. indica and O. rufipogon. Furthermore, in cold tolerance at the plumule stage, the clinal variation across the latitude of origins was observed within
O. rufipogon and ssp. japonica, suggesting that the current pattern of variation seems to have been shaped by both their phylogenetic histories and on-going
adaptation to the local environments. QTL analysis between O. sativa ssp. japonica (tolerant) and O. rufipogon (susceptible) revealed five putative QTLs for cold tolerance at the plumule and seedling stages but not at the germination
stage. Substitution mapping was also carried out to precisely locate the two major QTLs for cold tolerance at the plumule
stage, which could be used for improvement of tolerance to cold stress in ssp. indica. 相似文献
93.
Tomohiro Nishizono Kunihiro Tanaka Kazuo Hosoda Yoshio Awaya Yasuhiko Oishi 《Journal of Forest Research》2008,13(5):264-274
We analyzed data from 28 long-term experimental monitoring plots installed in Japanese cedar (Cryptomeria japonica D. Don) plantations in northeastern Japan to examine how site productivity and thinning practices relate to culmination in
stand growth. Site productivity and thinning practices in the plots were evaluated by site index (dominant tree height at
40-years old) and by cumulative thinning rate (cumulative thinning volume divided by cumulative gross production during the
entire period of measurement). Culmination of stand growth was evaluated by culmination age of the mean annual increment (MAI)
and its maximum value (Max MAI). Max MAI for the mean annual gross increment (MAIgross) and mean annual net increment (MAInet)
increased with increasing site index, but did not change with cumulative thinning rate. Culmination age for MAIgross decreased
with increasing site index, but did not change with cumulative thinning rate. Culmination age for MAInet decreased with increasing
site index. Additionally, culmination age for MAInet increased with increasing cumulative thinning rate in sites with a high
site index (>19.3 m) but not in those with a low site index (<19.3 m). These results indicate that thinning extends the culmination
age without changing Max MAInet under high site productivity. Therefore, thinning increases total net yield in sites with
high productivity based on a long-term perspective. 相似文献
94.
Somfai T Inaba Y Aikawa Y Ohtake M Kobayashi S Konishi K Nagai T Imai K 《Acta veterinaria Hungarica》2010,58(4):465-474
The aim of the present study was to optimise the culture conditions for the in vitro production of bovine embryos. The development of in vitro fertilised bovine oocytes in CR1aa supplemented with 5% calf serum and IVD101 culture media were compared using traditional microdrops and Well of the Well (WOW) culture systems either under 5% or 20% oxygen tension. After 7 days of culture, a significantly higher blastocyst formation rate was obtained for embryos cultured in CR1aa medium compared to those cultured in IVD101, irrespective of O2 tensions and culture systems. The blastocyst formation in IVD101 was suppressed under 20% O2 compared to 5% O2 . Despite their similar total cell numbers, higher rates of inner cell mass (ICM) cells were observed in blastocysts developed in IVD101 medium than in those developed in CR1aa, irrespective of O2 tensions. There was no significant difference in blastocyst formation, total, ICM and trophectoderm (TE) cell numbers between embryos obtained by microdrop and WOW culture systems irrespective of the culture media and O2 tensions used. In conclusion, CR1aa resulted in higher blastocyst formation rates irrespective of O2 tension, whereas IVD101 supported blastocyst formation only under low O2 levels but enhanced the proliferation of ICM cells. 相似文献
95.
Kazuyuki Maeda Yoshio Kurahashi Shuichi Ohsato Katsuyoshi Yoneyama 《Journal of General Plant Pathology》2010,76(5):303-309
An unknown disease abruptly appeared on hydroponic cultures of common ice plant (Mesembryanthemum crystallinum) in the greenhouse, causing catastrophic damage. Although the symptoms of the plant were unlike typical Botrytis lesions on leaves and stems of other plants, fungi isolated from the necrotic lesions on the plant were similar to genus
Botrytis in terms of conidial shape, colony color and nature. A representative isolate, Ice-2, caused similar symptoms on the host plants after inoculation with conidia, and the same
fungus was isolated from the lesions. The conidia and conidiophores of Ice-2 were morphologically similar to those of B. cinerea but not to those of B.
allii, B. fabae or B. squamosa. The tested Botrytis fungi grew at temperatures between 5 and 30°C. Ice-2 grew faster than the others at the lower end of the temperature range.
Ice-2 was also more virulent than B. cinerea (Bay-1) in artificial inoculations, especially on common ice plant leaves. The glyceraldehyde-3-phosphate dehydrogenase gene
(G3PDH) sequence of Ice-2 was determined and compared with those from four Botrytis species. The gene sequence of Ice-2 appeared to be identical to that of B. cinerea. In leaf tests on common ice plant and kidney bean, the diseases caused by Ice-2 and Bay-1 were controlled equally well by
the primary Botrytis fungicides. Based on the results of the present studies, Ice-2 is thought to be Botrytis cinerea Person: Fries. 相似文献
96.
SIQIN Mari NAKAI Tomohiro HAGI Shinichi KATO Ali Mohammed PITIA Mai KOTANI Yuki ODANAKA Yasushi SUGAWARA Koh‐Ichi HAMANO Keiichiro YOGO Yoshio NAGURA Masaru FUJITA Hiroshi SASADA Eimei SATO Tetsuya KOHSAKA 《Animal Science Journal》2010,81(6):681-686
Relaxin‐like factor (RLF), also known as insulin‐like factor 3 (INSL3), is produced by testicular Leydig cells, but its specific receptor LGR8 (leucine‐rich repeat family of G‐protein‐coupled receptor 8) has not been identified in goats. This study aimed to identify complementary DNA (cDNA) sequences of goat LGR8, and characterize the expression of both RLF and LGR8 in goat testes by RT‐PCR and immunohistochemistry. Testes were collected from immature (3‐month‐old) and mature (24‐month‐old) Saanen goats, and partial cDNA sequences of the goat homologue of human LGR8 were identified. The sequence encoded a reduced peptide sequence of 167 amino acids, which corresponded to transmembrane regions 2 through 5, followed by the beginning of intracellular loop 3 of human LGR8. Expression of both LGR8 and RLF genes was drastically increased in mature testes compared with immature ones. Although RLF protein was restricted to Leydig cells, LGR8 protein was detected in both Leydig cells and seminiferous epithelial cells (possibly germ cells and Sertoli cells). These results reveal a possible existence of the RLF‐LGR8 ligand‐receptor system within the goat testis, suggesting that RLF may play a role in testicular function through LGR8 on Leydig cells and seminiferous epithelial cells in an autocrine and/or paracrine manner. 相似文献
97.
98.
The purpose of this study was to demonstrate the inhibitory effect of chemicals on methane emissions in paddy soil. We found that (4-hydroxyphenyl) chloromethanesulfonate (C-1) has a methanogenic inhibition activity, and we studied its inhibition mechanism using laboratory tests. The study found that C-1 treatment of flooded soil did not significantly affect the bacterial community but rather the archaeal community; particularly, Methanosarcina spp. C-1 strongly inhibited the aceticlastic methanogenesis route. It was suggested that the inhibitory target of C-1 was different from the well-known methanogenic inhibitor 2-bromoethanesulfonate, which targets methyl-coenzyme M reductase of methanogen. In addition, C-1 had a secondary effect of inhibiting the dechlorination of chlorophenols. Although field trials are required as the next development step, C-1 can be used to reduce methane emissions from paddy fields, one of the largest sources in the agricultural sector. 相似文献
99.
Shoko NAKAMUTA Yoshio YAMAMOTO Nobuaki NAKAMUTA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(7):885
In the olfactory organ of lungfish, recesses at the bases of lamellae comprise sensory and nonsensory epithelia. The sensory epithelium of the recesses, the recess epithelium, is distinguished from the olfactory epithelium covering the lamella by the absence of ciliated olfactory receptor cells. Therefore, it has been suggested that the recess epithelium is a primordium of the vomeronasal organ of tetrapods. However, developmental changes in the number and distribution of recesses in the olfactory organ of lungfish were unknown. We examined four Protopterus aethiopicus specimens of body lengths 215–800 mm to determine the localization of recesses in their olfactory organs. Histological examination showed recesses at the bases of lamellae in all individuals examined. The recesses were localized mainly in the medial and caudal parts of the olfactory organs, especially in juveniles. Compared to smaller fish, larger fish had a larger number of recesses, distributed more broadly in their olfactory organs. Significance of the recess localization and its relationship to the function of lungfish olfactory organ warrants further investigation. 相似文献
100.
Miyake M Yaguchi T Saze K Wang J Ogawa T Endo Y Suzuta Y Okazaki M Haga Y Waki T Takahashi SY Yamamoto Y Iwabuchi S 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(1):9-14
Hepatocyte growth factor (HGF) is a pleiotropic cytokine that stimulates a wide array of cellular targets, including hepatocytes and other epithelial cells, melanocytes, endothelial and hematopoietic cells. We have cloned a different form of cDNA, with a deletion of 15 base pairs predicted to result in the loss of 5 amino acids from the first kringle domain. To investigate the biological activity, original and deleted variant of feline HGF cDNAs were transiently expressed in COS-7 cells. Both recombinant feline HGFs showed almost the same dose-response curves in the stimulation of the growth of BNL CL.2 cells (a mouse hepatocyte cell line) and scatter activity of Madin-Darby canine kidney (MDCK) cells. The findings reported here suggest that the deleted variant of feline HGF has almost the same biological activity as the original in terms of the proliferation and scatter activity. 相似文献