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排序方式: 共有156条查询结果,搜索用时 15 毫秒
111.
Yamada M Nakamura K Saido-Sakanaka H Asaoka A Yamakawa M Yamamoto Y Koyama Y Hikosaka K Shimizu A Hirota Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2005,67(10):1005-1011
Anti-bacterial activity of two synthesized oligopeptides, RLYLRIGRR-NH2 (peptide A) and RLRLRIGRR-NH2 (peptide B), both which based on a putative active site of defensin, an anti-bacterial peptide from the beetle Allomyrina dichotoma, was examined by macroscopic and histopathologic assessment during the course of infection in mice inoculated with methicillin-resistant Staphylococcus aureus (MRSA) in vivo. Both peptides A and B decreased the mortality of mice inoculated with MRSA. Peptides A and B decreased the macroscopical and histopathological lesions by MRSA infection in mice even seven days after the challenge. The anti-bacterial activity of peptides A and B has a therapeutic effect on MRSA infection in mice even seven days after being challenged. 相似文献
112.
Fuki Kawaguchi Yuto Kitamura Ryuji Nakajima Masayoshi Takahashi Hiroaki Goto Yuichi Washida Yoshikazu Yamamoto Shinji Sasazaki Hideyuki Mannen 《Animal Science Journal》2018,89(1):257-258
The objective of this study was to discriminate between original Japanese and Australian Wagyu beef, which is sold in the Singapore markets, using six previously developed DNA markers. To effectively evaluate the six markers for breed identification, the probability of identification as Australian Wagyu beef was calculated based on the estimated allele frequencies using 130 Australian Wagyu individuals. The combined use of six markers would allow the discrimination of Australian Wagyu beef with an estimated probability of 0.776. The probability to discriminate Australian Wagyu from Japanese Wagyu beef was sufficiently high. In addition, Australian Wagyu has maternal mitochondrial DNA of Bos indicus cattle with moderate high frequency of 0.377. The DNA marker system could also be used as a deterrent force against false sales, and contribute to the reduction and prevention of incorrect or falsified labeling of beef. 相似文献
113.
Kenji Takeda Hiromi Hara Naohiko Kobayashi Kazuo Kita Yoshikazu Sugimoto Kei Hanzawa 《Animal Science Journal》2016,87(9):1178-1181
Isoleucyl‐tRNA synthetase (IARS) c.235G > C (p.V79L) is a causative mutation for a recessive disease called IARS disorder in Japanese black cattle. The disease is involved in weak calf syndrome and is characterized by low birth weight, weakness and poor suckling. The gestation period is often slightly extended, implying that intrauterine growth is retarded. In a previous analysis of 2597 artificial insemination (AI) procedures, we suggested that the IARS mutation might contribute toward an increase in the incidence of prenatal death. In this study, we extended this analysis to better clarify the association between the IARS mutation and prenatal death. The IARS genotypes of 92 animals resulting from crosses between carrier (G/C) × G/C were 27 normal (G/G), 55 G/C and 10 affected animals (C/C) (expected numbers: 23, 46 and 23, respectively). Compared to the expected numbers, there were significantly fewer affected animals in this population (P < 0.05), suggesting that more than half of the affected embryos died prenatally. When the number of AI procedures examined was increased to 11 580, the frequency of re‐insemination after G/C × G/C insemination was significantly higher at 61‐140 days (P < 0.001). The findings suggested that the homozygous IARS mutation not only causes calf death, but also embryonic or fetal death. 相似文献
114.
Expression of inflammatory-related factors in porcine anterior pituitary-derived cell line 总被引:2,自引:0,他引:2
Nagai Y Ogasawara H Taketa Y Aso H Kanaya T Miyake M Watanabe K Ohwada S Muneta Y Yamaguchi T 《Veterinary immunology and immunopathology》2008,124(3-4):201-208
Recent studies have shown that undifferentiated stem cells act as immunomodulators. To investigate the immunomodulatory function of the progenitor cells of the anterior pituitary gland, we attempted to establish a stem/progenitor cell line from the porcine anterior pituitary gland, and to detail its inflammatory cytokine expression. A cloned cell line from the porcine anterior pituitary gland was established and was designated as the porcine anterior pituitary-derived cell line (PAPC). PAPC expressed the mRNA of Nanog and Oct-4, and showed positive immunoreactivity for beta-catenin and Hes1 in its nucleus. PAPC grew stably by repeated passage and rapidly in the EGF and bFGF containing medium. RT-PCR showed that PAPC expressed mRNA of IL-1alpha, IL-6, IL-12, IL-15, IL-18 and TLR4. PAPC expressed S100alpha and IL-18 protein, which was localized in the marginal epithelial cells of Rathke's pouch. These results suggest that PAPC is a stem/progenitor cell and may regulate anterior pituitary cell function through an immuno-endocrine pathway. 相似文献
115.
116.
Shuhei HIDAKA Mitsutoshi KOBAYASHI Kunihide ANDO Yoshikazu FUJII 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(7):829-835
Lomefloxacin is a broad-spectrum fluoroquinolone antibiotic used for the treatment of
bacterial extraocular disease. This study investigated the efficacy and safety of
lomefloxacin eye drops for bacterial extraocular disease in horses. Lomefloxacin
ophthalmic solution (0.3%) was instilled three times daily for 2–5 days in 65 horses
diagnosed with bacterial extraocular disease based on clinical findings. Clinical
observations and bacteriological examinations were performed at the start of treatment, 2
and 5 days after the start of treatment, and at the discontinuation or termination of
treatment. Of the 65 horses, 64 were positive for bacteria, and 22 bacterial genera and 47
bacterial species were identified. The efficacy of lomefloxacin was evaluated in 63
horses; one horse with a negative culture and another with suspected bacterial
contamination were excluded. Lomefloxacin was considered to be clinically effective in 54
horses. The major bacterial species identified were Staphylococcus
aureus, Streptococcus equi subsp. zooepidemicus,
Acinetobacter lwoffii, Staphylococcus xylosus,
Staphylococcus vitulinus, Enterobacter agglomerans,
Flavimonas oryzihabitans and Staphylococcus sciuri,
with a cumulative disappearance rate of 80% or more at the termination of instillation.
Excluding one horse that did not undergo a bacteriological examination, the remaining 62
horses were assessed for bacteriological outcome. Full or partial bacterial clearance was
detected in 95% or more of the 62 horses. One of the 65 horses reported adverse events
that had no causal relation with the eye drops. Our results showed that lomefloxacin is
safe and effective for the treatment of bacterial extraocular disease in horses. 相似文献
117.
Kazuko OGATA Aiko SASAKI Yuka KATO Arisa TAKEDA Mikio WAKABAYASHI Borjigin SARENTONGLAGA Mio YAMAGUCHI Asuka HARA Rika FUKUMORI Yoshikazu NAGAO 《The Journal of reproduction and development》2015,61(2):116-122
The present study was conducted to evaluate whether supplementation of semen extender with glutathione (GSH) can maintain the quality of frozen-thawed canine spermatozoa. Eighteen ejaculates were obtained from 5 dogs and placed in extender (20% egg yolk, Tris, citric acid, lactose, raffinose, antibiotics and 6.5% glycerol) containing 0 (control), 2.5, 5, 7.5 or 10 mM GSH. The samples were cooled to 4 C and then frozen in liquid nitrogen vapor. Motility parameters of the sperm were evaluated at 0, 1, 2, 3, 4, 12 and 24 h after thawing. Sperm motility was higher in the 5 mM GSH group than in the control or 2.5 and 10 mM GSH groups; this effect was observed at 1 to 24 h after thawing (P < 0.05). The 5 mM GSH group had a higher sperm viability index at 12 and 24 h after thawing compared with the other groups (P < 0.05). Acrosome integrity, evaluated at 4 h after thawing, was greater in two of the GSH-treated groups (5 and 10 mM) compared with the control. Lipid
peroxidation (LP) levels immediately after thawing were lower in the 5 and 10 mM GSH groups compared with the control, while those at 12 h after thawing did not differ significantly. Frozen-thawed semen in the 5 mM GSH group was used for transcervical insemination of 4 bitches, resulting in delivery of 5 puppies from 2 bitches. These results indicate that supplementation of semen extender with 5 mM GSH was effective in improving motility, longevity and acrosomal integrity and inhibiting LP levels in post-thaw canine spermatozoa, without any adverse impacts on full-term development after transcervical insemination. 相似文献
118.
Roudkenar MH Bouzari S Kuwahara Y Roushandeh AM Baba T Oloomi M Fukumoto M 《Iranian Biomedical Journal》2008,12(1):1-6
BACKGROUND: Immunotoxins are comprised of both the cell targeting and the cell killing moieties. We previously established a new immunotoxin, i.e. Shiga toxin granulocyte macrophage-colony stimulating factor (StxA1-GM-CSF), comprises of catalytic domain of Stx, as a killing moiety and GM-CSF, as a cell targeting moiety. In this study, the ability of the immunotoxin to induce apoptosis and double strand breaks (DSB) on different cell lines was investigated. METHODS: The recombinant hybrid protein was expressed in bacterial expression system and purified with nickel-nitrilotriacetate acid resin. The K562 (erythroid leukemia) cell line and LS174 (colon carcinoma) were used in this study. The neutral comet assay was carried out for the detection of DSB and Hoechst staining was performed for apoptosis. RESULTS: StxA1-GM-CSF effectively induced apoptosis on K562 cell line and DNA Double Strand Break (DSB) were observed on colon cancer cell line treated with StxA1-GM-CSF. CONCLUSION: This novel action i.e. DNA damage might be a relevant mechanism of action for StxA1-GM-CSF that is designed to act as immunotoxin, although further investigation is required. 相似文献
119.
Haruka Nishikawa Ichiro Yasuda Sachihiko Itoh Kosei Komatsu Hideharu Sasaki Yoshikazu Sasai Yoshioki Oozeki 《Fisheries Oceanography》2013,22(6):509-522
Particle‐tracking experiments were performed to infer the distribution of larvae of the Japanese sardine (Sardinops melanostictus) and to detect effects of transport environment on sardine recruitment, using the output of a high‐resolution ocean general circulation model and observed data of sardine spawning grounds during 1978–2004. By the 60th day following spawning, approximately 50% of the larvae had been transported to the Kuroshio Extension (KE). Whereas the spawning period and grounds changed markedly in relation to the stock level, the proportion of larvae transported to the KE remained relatively constant and no significant correlations were found between sardine recruitment and the transport proportion. Instead, the recruitment was found to be correlated with physical parameters including the mixed layer depth and the sea surface temperature along several major transport trajectories of sardine larvae. The correlations were most significant for the trajectories in the region 0.5° south to 1° north of the Kuroshio axis (defined as the location of velocity maxima at each longitude) and for larvae spawned in February and March during the high stock period (1978–94), and for larvae spawned in March and April during the low stock period (1995–2004). 相似文献
120.
Yuta KINOSHITA Hironaga KAKOI Taichiro ISHIGE Takashi YAMANAKA Hidekazu NIWA Eri UCHIDA-FUJII Toshio NUKADA Takanori UENO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(1):129
Taylorella equigenitalis causes contagious equine metritis. Here we compared seven nucleic acid amplification tests for T. equigenitalis to select a rapid and reliable diagnostic method. The 95% detection limits of each assay varied greatly: real-time PCR had the lowest detection limit (0.77 fg/reaction); those of some of the conventional PCRs (cPCRs) were >100 fg/reaction. In experimentally infected samples, real-time PCR and semi-nested PCR showed the highest positive numbers (33 out of 42 samples), but two of the cPCRs detected only 2 and 7 positive results. Our results indicate that the use of sensitive molecular assays is important for the efficient detection of T. equigenitalis in clinical samples. 相似文献