Effect of phosphogypsum application on the growth and Ca uptake of Komatsuna Brassica rapa L. cv. Natsurakuten

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Application of phosphogypsum PG and limestone to Andisol were compared in terms of their effects on the growth and uptake of Ca in Komatsuna Brassica rapa L. cv. Natsurakuten. The studies were carried out in soil pot cultures, and both PG and limestone were applied to the Andisol at the rates of 0, 0.30, 0.75, 1.50 g kg⁻¹ in the form of CaO.

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  Limestone application resulted in an increase in the soil pH. On the other hand, no change was observed in the case of PG application at pH 6.1 ± 0.1 regardless of the application rate. Both soil EC and water-soluble Ca increased in proportion to the PG application rate, and this increase was approximately 5 times greater than that observed when limestone was applied at 1.50 g kg⁻¹. The w-Ca/ex-Ca ratio (water-soluble Ca: 1 M acetic acid-extractable Ca) was clearly increased from 0.12 up to 0.26 in PG, while the value was slightly decreased from 0.12 to 0.05 in limestone.

       

黑龙江省主栽水稻品种抽穗模型研究初报

采用黑龙江省生产上主栽的早熟、中熟和晚熟的6个水稻品种,在哈尔滨地区做分期栽培田间试验。调查记载播种、插秧和抽穗期。采取平行观测的气象资料,分析温度对水稻抽穗期和水稻营养生长期的影响。结果表明:不同的插秧期水稻抽穗期变化很大,不同熟期的水稻品种插秧至抽穗期需要的有效积温差别明显,但每个品种需要的有效积温很稳定。     

Expression of podoplanin in various types of feline tumor tissues

Podoplanin is expressed in various human tumors where it promotes tumor progression, epithelial-mesenchymal transition, and distant metastasis. Podoplanin is also expressed in cancer-associated fibroblasts and induces tumor malignancy. The objective of this study was to evaluate podoplanin expression in various types of feline tumor tissues. Immunohistochemical analysis revealed that podoplanin was expressed in cells of 13/15 (87%) squamous cell carcinomas and 5/19 (26%) fibrosarcomas. Moreover, cancer-associated fibroblasts expressed podoplanin in most tumor types, including 18/21 (86%) mammary adenocarcinoma tissues. Our findings demonstrate that various types of feline tumor tissues expressed podoplanin, indicating the importance of the comparative aspects of podoplanin expression, which may be used as a novel research model for podoplanin biology.     

Cloning of estrogen receptor-β cDNA of common Japanese conger Conger myriaster and expression of the gene during oocyte development

ABSTRACT:   Estrogen receptor (ER) cDNA was cloned from the liver of common Japanese conger Conger myriaster . Common Japanese conger ER cDNA contains 2543 nucleotides including an open reading frame that encodes 596 amino acids, with a molecular weight of 66.1 kDa. The deduced amino-acid sequence of the common Japanese conger ER shares 85% identity with that of eel Anguilla japonica ER-β, and 39–46% and 53–59% identity with the deduced sequences of ER-α and ER-β of other species, respectively. In a phylogenetic analysis, the common Japanese conger ER was found to cluster with the ER-β of other species. The common Japanese conger ER-β also has six domains (A–F), which are conserved in the nuclear receptor superfamily. The amino-acid sequences in the C and E domains were highly conserved between common Japanese conger ER-β and other ER. However, the other domains showed low homology. In the female common Japanese conger, ER-β mRNA was highly expressed in the liver and pituitary gland. The expression levels of the ER-β gene increased from the oil droplet stage to the primary yolk globule stage and were maintained until the migratory nucleus stage.     

Evaluation of Low Level Laser Therapy on Primary Healing of Experimentally Induced Full Thickness Teat Wounds in Dairy Cattle

Objective —The purpose of this study was to evaluate the effect of low-level laser therapy (LLLT) on sutured wounds of the teat in dairy cattle.
Study Design —By using the Latin square design, the effect of LLLT was evaluated by radiography, measurement of microcirculation flow, histopathology, tensiometry, and hydroxyproline analysis.
Animals or Sample Population —Sixteen teats of four dairy cattle.
Methods —Full thickness wounds were made on the cranial surface of the teats. Teats were distributed into four groups; group A and B wounds were closed with a Gambee pattern, group C and D wounds were closed with three-layers of continuous suture pattern. Group B and D wounds were treated with 3.64 J/cm² of LLLT using a helium-neon system continuous wave (632.8 nm) output of 8.5 mW.
Results —The teat wall in non-LLLT groups was significantly thicker than in LLLT groups on day 7, 14 and 21. The mean blood flow differences between control and sutured sites in LLLT groups were significantly lower than those in non-LLLT groups. The morphology of the epidermis in LLLT groups more closely resembled the normal epidermis than that of non-LLLT groups. Collagen fibers in LLLT groups were denser, thicker, better arranged and more continuous with existing collagen fibers than those in non-LLLT groups. The mean tensile strength was significantly greater in LLLT groups than in non-LLLT groups.
Conclusion —The LLLT affects various aspects of the healing process, including minimizing inflammation, formation of edema, improvement of skin regeneration and enhancement of collagen synthesis.
Clinical Relevance —The LLLT could accelerate healing of sutured wounds of the teat in dairy cattle.     

VIRTUAL ENDOSCOPY OF DOGS USING MULTI-DETECTOR ROW CT

Virtual endoscopy uses CT data to display hollow viscera such as the stomach, duodenum and colon as if by real endoscopic observation. The results are independent of the operator skill because virtual endoscopy does not require direct manipulation of endoscopic equipment. The purpose of this study was to evaluate the utility of virtual endoscopy in small animals. Ten beagles were examined by conventional endoscopy and virtual endoscopy using multidetector CT. Virtual images were image-processed at a workstation designed for depicting virtual endoscopy using CT data. Virtual images were visually comparable with conventional endoscopic images, especially in the area of the angular incisure and pylorus. The advantages of virtual endoscopy are observation from any viewing angle, a reduced learning curve for diagnostic interpretation, quantification of lesion size in three dimensions and a potential for reducing anesthesia time. The disadvantages of virtual endoscopy are a lack of color recognition information, an inability to retrieve biopsy samples, limited visualization of the duodenum, artifacts from respiration/peristalsis motion and an inability to observe function. Based on our results, we suggest that conventional endoscopy is still superior to virtual endoscopy in dogs. However, as technology improves, virtual endoscopy may emerge as a suitable alternative or adjunctive diagnostic tool for certain digestive disorders in small animals.