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Yoichi INOUE Tetsushi ONO Keiichi HISAEDA Yutaka YAMADA Akihisa HATA Takako Shimokawa MIYAMA Kenichi SHIBANO Emi OHZAWA Hitoshi KITAGAWA Eri IWATA 《Journal of Equine Science》2022,33(2):27
The objective of this study is to analyze the relationships between the age and blood test results or body sizes in Noma horses by using the results of periodical health examination. Out of 45 hematological or physical items examined, statistically significant, but loose correlations were observed in 14 items. Red blood cell count, activities of aspartate aminotransferase, alkaline phosphatase, and creatinine kinase, concentrations of calcium and inorganic phosphorus decreased with aging. Conversely, mean corpuscular volume, mean corpuscular hemoglobin, lipase activity, γ-globulin and chloride concentrations, body height, chest circumference and cannon bone circumference increased with aging. The changes in a few items seemed unique to Noma horse. However, most age-related changes found in this study might be considered as a common trend in horse breeds rather than distinctive characteristic in Noma horse. 相似文献
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Naoki SASAKI Nao TSUZUKI Michiaki YAMADA Takuto MINAMI Haruo YAMADA 《Journal of Equine Science》2009,20(1):7-10
This study aimed to establish a standard dose and sample collection time for
13C phenylalanine and 13C-Dipeptide breath test in horses. To
evaluate dose-dependent effects, healthy horses received 2.5 mg/kg, 5 mg/kg, and 10 mg/kg
13C phenylalanine dissolved in 1 ml/kg distilled water and 1.25 mg/kg, 2.5
mg/kg, and 5 mg/kg 13C dipeptide dissolved in 2 ml/ kg distilled water. Tmax
was observed during the sample collection time. For 13C phenylalanine, the
standard deviation of Cmax at 5 mg/kg was lower than that of 10 mg/kg. For 13C
dipeptide, the standard deviation of Tmax was the lowest at 5 mg/kg. This study revealed
that an optimal dose for breath tests with 13C phenylalanine and 13C
dipeptide may be 5 mg/kg in horses. 相似文献
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Manabu YAMADA Kentaro MASUJIN Ken-ichiro KAMEYAMA Reiko YAMAZOE Takashi KUBO Kei IWATA Aiko TAMURA Hiroyuki HIBI Takayoshi SHIRATORI Shunjiro KOIZUMI Kousuke OHASHI Mitsutaka IKEZAWA Takehiro KOKUHO Makoto YAMAKAWA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2020,82(12):1835
We experimentally infected pigs with the African swine fever virus (ASFV) Armenia 07 strain (genotype II) to analyze the effect of different dose injections on clinical manifestations, virus-shedding patterns, histopathology, and transmission dynamics by direct contact. Each three pigs and four pigs were injected intramuscularly with 0.1 fifty percent hemadsorbing doses (HAD50)/ml, 101 HAD50/ml and 106 HAD50/ml of ASFV Armenia 07 strain, respectively. Each two of three pigs injected with 0.1 HAD50/ml and 101 HAD50/ml died by 10 days post inoculation. All pigs had a gross lesion of splenomegaly. Perigastric and renal lymph nodes were enlarged and resembled blood clots in nine of ten pigs. It was revealed that 0.1 HAD50/ml of this ASFV was sufficient to infect healthy pigs by intramuscular injection and caused sub-acute lethal disease. For the transmission study, two 8-week-old pigs were injected intramuscularly with 103 HAD50/ml of the same virus. Each of the experimentally inoculated pigs was co-housed with two 8-week-old naive pigs. All contact pigs exhibited clinical manifestations at 6 or 7 days after the experimentally inoculated pigs developed pyrexia. These findings suggest that this strain may spread slowly within a herd. Histologically, lymph nodes resembled blood clots were formed by severe blood absorption and followed hemorrhage result of disruption of the lymphoid sinus filling with absorbed red blood cells. The severity of the gross and histological lesions depended on duration after infection, regardless of the difference of injection doses in this study. 相似文献
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Salinity stress is a major factor limiting the growth of turfgrass irrigated with recycled wastewater. The change in lipid peroxidation in terms of malondialdehyde (MDA) content and the activities of superoxide dismutase (SOD), catalase (CAT), ascorbate peroxide (APX) and glutathione reductase (GR) in the shoots and roots of Kentucky bluegrass and tall fescue were investigated under salinity stress. Plants were subjected to 0, 50, 100, 150 and 200 mmol L 1 NaCl for 40 d. The MDA content under salinity stress was lower in tall fescue than in Kentucky bluegrass in both shoots and roots. Activities of SOD in the shoots of both species increased with salinity stress. The activities of CAT and APX decreased in Kentucky bluegrass, but no significant difference in the activities of CAT and APX was observed in tall fescue. The activities of SOD, CAT and APX in the shoots of tall fescue were higher than those in Kentucky bluegrass. In the roots of Kentucky bluegrass, SOD and GR activities increased and CAT and APX activities decreased in comparison with the control. In the roots of tall fescue, salinity increased the activities of SOD, CAT, and APX. These results suggested that tall fescue exhibited a more effective protection mechanism and mitigated oxidative stress and lipid peroxidation by maintaining higher SOD, CAT and APX activities than Kentucky bluegrass. 相似文献
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Oogenesis in the common Japanese conger Conger myriaster 总被引:1,自引:0,他引:1
TOMOKO UTOH NORIYUKI HORIE AKIHIRO OKAMURA YOSHIAKI YAMADA SATORU TANAKA NAOMI MIKAWA ATSUSHI AKAZAWA HIDEO P OKA 《Fisheries Science》2003,69(1):181-188
ABSTRACT: We investigated the process and characteristics of oogenesis in the common Japanese conger Conger myriaster . Young fish caught in November 1996 were reared for use in this experiment. Fish were sampled monthly from December 1997 to August 1998. Some were injected with human chorionic gonadotropin to stimulate ovarian maturation from May to August 1998. Oocytes from the chromatin nucleolus stage to the secondary yolk globule stage were obtained from non-hormone-treated fish; those of more advanced stages were obtained from hormone-treated ones. We divided oocyte development into eight stages from the chromatin nucleolus stage to the maturation stage. The yolk vesicle stage was not separated because yolk vesicles began to appear just after appearance of yolk globules. Oocyte, oil droplet, yolk globule and nucleus diameters all increased concomitant with oocyte development. Oil droplet and yolk globule diameters increased remarkably at the maturation stage. However, zona radiata thickness peaked at the secondary yolk globule stage, decreasing gradually thereafter. Increased gonadosomatic index was related to oocyte development as found in European and Japanese eels receiving hormone treatment to mature. The present study is the first report describing oogenesis characteristics in congrid eels. It indicates that oogenesis is almost identical to that of other anguillid eels. 相似文献
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