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21.
Kittaka-Katsura H Fujita T Watanabe F Nakano Y 《Journal of agricultural and food chemistry》2002,50(17):4994-4997
Vitamin B(12) content of an algal health food, Chlorella tablets (Chlorella sp.), was determined by both Lactobacillus leichmannii ATCC 7830 microbiological and intrinsic factor-chemiluminescence methods. The values of 200.9-211.6 microg/100 g dry weight determined by the chemiluminescence method were similar to the values (201.3-285.7 microg/100 g dry weight) determined by the microbiological method. A corrinoid compound was purified to homogeneity from the Chlorella tablets and characterized. The purified corrinoid compound was identified as vitamin B12, on the basis of silica gel 60 TLC, C18 reversed-phase HPLC, 1H NMR spectroscopy, and UV-Vis spectroscopy. 相似文献
22.
A phase transition from a classical thermal mixed state to a quantum-mechanical pure state of exciton polaritons is observed in a GaAs multiple quantum-well microcavity from the decrease of the second-order coherence function. Supporting evidence is obtained from the observation of a nonlinear threshold behavior in the pump-intensity dependence of the emission, a polariton-like dispersion relation above threshold, and a decrease of the relaxation time into the lower polariton state. The condensation of microcavity exciton polaritons is confirmed. 相似文献
23.
Kittaka-Katsura H Ebara S Watanabe F Nakano Y 《Journal of agricultural and food chemistry》2004,52(4):909-911
A Japanese fermented black tea (Batabata-cha) contained a considerable amount of vitamin B(12) (456 +/- 39 ng per 100 g dry tea leaves and 2.0 +/- 0.3 ng per 100 mL of tea drink). A corrinoid compound was partially purified and characterized from the tea leaves. The patterns of the purified compound by the silica gel 60 thin-layer chromatography and C18 reversed phased high-performance liquid chromatography were identical to those of authentic vitamin B(12). When 20 week old vitamin B(12) deficient rats, which excreted substantial amounts (about 250 mg/day) of methylmalonic acid in urine as an index of vitamin B(12) deficiency, were fed the tea drink (50 mL/day, 1 ng of vitamin B(12)) for 6 weeks, urinary methylmalonic acid excretion (169 +/- 29 mg/day) of the tea drink-supplemented 26 week old rats decreased significantly relative to that (250 +/- 32 mg/day) of the deficient rats. The results indicate that the vitamin B(12) found in the fermented black tea is bioavailable in mammals. 相似文献
24.
Morifusa Eto Sachiko Okabe Yoshihisa Ozoe Kazuyuki Maekawa 《Pesticide biochemistry and physiology》1977,7(4):367-377
O,S-Dimethyl phosphoramidothiolate, the insecticide methamidophos, is converted oxidatively into a potent anti-cholinesterase agent by the action of m-chloroperbenzoic acid. The active intermediate is not stable enough to be isolated. From some chemical evidence, the sulfoxide, i.e., dimethyl phosphoramidothiolate S-oxide, is most probably the active intermediate. A similar activation may occur by biological oxidation. 相似文献
25.
Shimamura S Shimizu M Kobayashi M Hirao H Tanaka R Yamane Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2005,67(9):935-937
A seven-year-old, female, domestic short hair cat was presented with a history of chronic anorexia. Radiographic examination revealed a large space-occupying calcified mass in the abdominal cavity. The mass was located in pylorus and did not extend into the duodenum and surrounding tissues. Billroth I gastroduodenostomy was conducted to remove the mass. Histopathological examination of the mass showed a lymphoma. Although Recovery following the operation was excellent, the patient showed intermittent vomiting unrelated to feeding. Radiographical examination revealed a megaesophagus, which was assumed to be a complication of the Billroth I procedure, since the condition was not observed before the procedure. 相似文献
26.
The effect of intermittent administration of sustained release isosorbide dinitrate (sr-ISDN) in rats with volume overload heart 总被引:1,自引:0,他引:1
Shimamura S Ohsawa T Kobayashi M Hirao H Shimizu M Tanaka R Yamane Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(1):49-54
Recently, it has been reported that intermittent administration of nitrate, with a nitrate-free interval of 10 to 12 hr eliminated expression of tolerance, and maintained its hypotensive effect. In the present study, we evaluated whether nitrate tolerance developed or not with an intermittent administration of sr-ISDN (5 mg/kg/ once a day) in Wistar rats. The effect of this administration protocol for sr-ISDN on the volume overload heart model, aortovenous fistula, was also examined. Furthermore, blood pressure was monitored by radio telemetry during sr-ISDN (5 mg/kg/once a day) administration. Nitrate tolerance did not develop, and eccentric hypertrophy due to volume overload was moderated by sr-ISDN administration. Sr-ISDN administration maintained blood pressure lower level than the placebo group. In conclusion, prolonged intermittent administration of sr-ISDN maintained its hypotensive effect during the entire experiment period, without developing tolerance, and moderated efferent hypertrophy with attenuated volume overload. 相似文献
27.
Shimizu M Tanaka R Uchida M Orito K Shimamura S Yamane Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(3):227-233
It has been reported that cardiac chymase has an effect on cardiac fibrosis through the Angiotensin (Ang) II formation and an Ang II-independent mechanism. In the present study, Ang II type 1 (AT1) receptor blocker (candesartan cilexetil) was administered to dilated cardiomyopathic (DCM; Bio TO2) hamsters for 4 weeks to study the effect of AT1 receptor blocker on cardiac chymase-like activity and cardiac fibrosis. Echocardiography, histological examination, and assessment of cardiac angiotensin-converting enzyme (ACE)/chymase-like activities were conducted. Hamsters showed cardiac dysfunction due to increased left ventricular dimensions and decreased ventricular wall thickness, significant increase in cardiac chymase-like activity, and fibrosis. This result indicates that the cardiac chymase-like activity is responsible for cardiac fibrosis. When candesartan cilexetil was administered to Bio TO2 hamsters, cardiac chymase-like activity increased significantly, whereas cardiac fibrosis decreased significantly. Cardiac ACE and chymase-like activities were unchanged in non-DCM hamsters with candesartan cilexetil. This suggests that the cardiac Ang II formation mechanism was stimulated by suppressing the effect of cardiac Ang II, and cardiac chymase-like activity could be increased. Moreover, this mechanism may be more highly activated if cardiac Ang II is activated in the heart. In conclusion, we demonstrated that AT1 receptor blocker reduced cardiac fibrosis, although cardiac chymase-like activity increased. Because the Ang II-forming pathway and the effect of chymase in hamsters is similar to that in dogs, the results of the present study may supplement the available information for dogs. 相似文献
28.
Ushiro Y Hashimoto O Seki M Hachiya A Shoji H Hasegawa Y 《The Journal of reproduction and development》2006,52(4):487-495
Activins, TGF-beta superfamily members, have multiple functions in a variety of cells and tissues. Additional activin beta subunit genes, betaC and betaE, have been identified in humans and rodents. To explore the role of activin betaC subunit, we generated recombinant human activin C using Chinese hamster ovary cells. Recombinant activin C from the conditioned medium was purified by consecutive hydrophobic, size-exclusion, and high performance liquid chromatography. SDS-PAGE and Western blot analysis of the purified protein revealed that activin C formed disulfide bridges. However, activin C had no effect on the proliferation of cultured liver cells. Furthermore, there were no significant differences in erythroid differentiation and follicle stimulating hormone secretion in vitro. It was also shown that immunoreactive bands indicated the hetrodimer of activin betaC, and inhibin alpha subunits were detected in the conditioned medium from the activin C-producing cells, which were stably transfected with inhibin alpha subunit cDNA. This suggests that activin betaC subunit may have been present and that it may exert its effect as inhibin C. 相似文献
29.
Byung-Jae Kang Hak-Hyun Ryu Sung Su Park Yoshihisa Koyama Masanori Kikuchi Heung-Myong Woo Wan Hee Kim Oh-Kyeong Kweon 《Journal of veterinary science (Suw?n-si, Korea)》2012,13(3):299-310
Alternative sources of mesenchymal stem cells (MSCs) for replacing bone marrow (BM) have been extensively investigated in the field of bone tissue engineering. The purpose of this study was to compare the osteogenic potential of canine MSCs derived from adipose tissue (AT), BM, umbilical cord blood (UCB), and Wharton''s jelly (WJ) using in vitro culture techniques and in vivo orthotopic implantation assays. After canine MSCs were isolated from various tissues, the proliferation and osteogenic potential along with vascular endothelial growth factor (VEGF) production were measured and compared in vitro. For the in vivo assay, MSCs derived from each type of tissue were mixed with β-tricalcium phosphate and implanted into segmental bone defects in dogs. Among the different types of MSCs, AT-MSCs had a higher proliferation potential and BM-MSCs produced the most VEGF. AT-MSCs and UCB-MSCs showed greater in vitro osteogenic potential compared to the other cells. Radiographic and histological analyses showed that all tested MSCs had similar osteogenic capacities, and the level of new bone formation was much higher with implants containing MSCs than cell-free implants. These results indicate that AT-MSCs, UCB-MSCs, and WJ-MSCs can potentially be used in place of BM-MSCs for clinical bone engineering procedures. 相似文献
30.
Takuro Hirai Kei Sawata Ali Awaludin Yoshihisa Sasaki Takeyoshi Uematsu 《Journal of Wood Science》2012,58(2):128-134
Shaking table tests of the wall-floor joints of wooden light-frame constructions under forced harmonic vibrations are conducted
in this study so as to observe the dynamic responsive characteristics. The principal results are as follows: The responsive
characteristics of timber constructions under strong earthquakes cannot be directly correlated with their resonant frequencies
under free or forced vibrations with low input accelerations, because they behave as continuous bodies when the input accelerations
are less than the apparent frictional limits of structural joints. The apparent frictional limits are reduced by periodic
fluctuation of the effective vertical loads as a result of the vertical motion of the specimens. The characteristic dynamic
responses of wall-floor joints depend clearly upon the frequency and input accelerations of forced vibrations. These dependencies
arise from the nonlinear load-slip relationship of the wall-floor joints. The equivalent stiffness in their successive transient
phases decreases as joint slip increases, which gradually changes the resonant frequencies of the wall-floor joints. This
indicates that the frequency components dominant to ultimate or safety-limit resistance should be distinguished from those
dominant to allowable or serviceability-limit resistance. 相似文献