Dimethylnitrosamine-induced liver fibrosis and recovery in NOD/SCID mice

There is a need for a new liver fibrosis model of immunodeficient mice to study the effects of cell therapy on liver disease because there are not many animal models available to study the effects of cell therapy. In this study, we induced liver fibrosis using dimethylnitrosamine (DMN) in NOD/SCID mice to create an animal model for liver disease. DMN (5 mg/kg, i.p.) was injected intraperitoneally for three consecutive days per week for 6 or 8 weeks, and the mice were sacrificed at weeks 0, 4 and 8 after the last DMN injection. The 6-week DMN-treated group gradually recovered from serum biochemical changes, histopathological toxic effects and lesions in the liver at weeks 4 and 8 after the last DMN injection. However, the progression of liver fibrosis and toxic levels were maintained in the 8-week DMN-treated group at week 4 after the last DMN injection. The increases in iron and extracellular matrix (collagen) in the DMN-treated group were confirmed by Prussian blue (PB) and Masson's trichrome (MT) staining, respectively. Additionally, activation of hepatic stellate cells was observed by alpha smooth muscle actin (α-SMA) immunostaining and western blot. In conclusion, treatment of NOD/SCID mice with 5 mg/kg of DMN for 8 weeks can be used to induce an appropriate animal model of disease for liver fibrosis. This model may be useful for evaluation of the efficacy and safety of cell therapies such as human mesenchymal stem cell therapy.     

中能中蛋白水平下维生素A、D3对0～6周龄肉仔鸡生产性能的影响

采用3×3随机交叉试验设计,研究中能中蛋白水平下日粮VA、VD3对肉仔鸡生产性能的影响。1日龄AA肉仔鸡540只随机分为9组,每组3个重复,每重复20只鸡。3个VA添加水平为3000、6000、12000IU/kg;3个VD3添加水平为200、400、800IU/kg;0～3和4～6周龄基础日粮代谢能、粗蛋白水平分别为12.34MJ/kg,21%;12.76MJ/kg,19%。结果表明:①日粮VA水平对体增重、耗料量、料重比、存活率均无显著影响(P>0.05);②随VD3添加水平的增加,体增重、耗料量、存活率显著增加(P<0.05);③综合考虑生产性能和经济效益,认为0～3和4～6周龄肉仔鸡日粮VA添加量均以3000IU/kg较为适宜,VD3添加量均以800IU/kg较为适宜。     

活禽交易对十堰市公共卫生安全的影响及对策

本文针对十堰市活禽养殖和交易的现状,结合工作实际,分析了活禽交易方式对公共卫生安全、禽流感防控、环境污染等方面的影响,以及活禽交易存在的问题及产生的原因,从政策建议、防疫条件、检疫监管、疫情监测预警、市场准入、可追溯体系建设、动物经纪人管理等方面提出了措施和建议,为进一步加强禽流感防控工作和动物产品质量安全管理提出了有益的探讨。     

松针褐斑病毒素诱发紫茎泽兰电解质渗漏对几种真菌的影响

松针褐斑病菌毒素是一种非寄主专化性毒素,能够伤害紫茎泽兰叶片,引起细胞电解质渗漏。电导率测定结果表明,毒素粗提液对紫茎泽兰的伤害活性最强,LA-I次之,LA-II最弱。孢子萌发试验和菌丝生长试验显示,紫茎泽兰电解质对青霉菌、木霉菌、尖孢镰刀菌、链格孢菌的孢子萌发和菌丝生长都有不同程度的抑制作用,但是对泽兰尾孢菌的孢子萌发和菌丝生长有促进作用。毒素粗提液对供试真菌的的孢子萌发和菌丝生长没有明显的抑制作用和促进作用。分析认为将松针褐斑病菌毒素与泽兰尾孢菌混和将可能提高对紫茎泽兰的除草效果。     

ULTRASONOGRAPHIC DIAGNOSIS—PYLOROGASTRIC INTUSSUSCEPTION IN A DOG

A 7-year-old female Maltese presented for evaluation of severe vomiting. A diagnosis of pylorogastric intussusception was made during ultrasonographic examination. The intussusception spontaneously underwent reduction by the following morning.     

蜂王浆的抗菌活性研究

本文采用平板培养法研究蜂王浆的抗菌活性 ,发现新鲜蜂王浆对10种试验菌均有不同程度的抑制作用。根据抑菌斑的大小判断 ,蜂王浆对各种试验菌株的抑制程度为 :枯草杆菌>威克氏酵母>蜡状芽孢杆菌>隐球酵母>鼠伤寒沙门氏菌>大肠杆菌O111>福氏志贺氏2型菌>假单孢杆菌>N1 米曲霉>霉菌W15。     

Spectral Doppler ultrasound in the major arteries of normal conscious immature micropigs

Spectral waveform analysis of blood flow velocity in the major arteries of six healthy, conscious immature micropigs was determined using Doppler ultrasonography. Doppler spectral tracings were recorded from the external iliac artery, femoral artery, and renal arcuate artery. Tracings were also taken from three parts of the common carotid artery and two parts of the abdominal aorta. Spectral Doppler parameters included peak systolic velocity, early diastolic velocity, peak systolic velocity-to-end diastolic velocity ratio, resistive index, and pulsatility index. In addition, the diameter of major arteries and indirect blood pressure were measured. These results from spectral Doppler analysis in major arteries may be useful as reference ranges in the future studies of vascular hemodynamics in immature micropigs.     

2006年第二季度大豆市场回顾与第三季度展望——大豆期货渴望攀高

1 行情回顾 2006年第二季度全球大宗商品市场演绎了一场波澜壮阔的完美风暴，以黄金、铜、原油为首的商品市场出现了疯狂的上涨，市场资金蜂拥而上，持仓、成交持续放大。大宗商品价格在天价区间运行。存在即合理，商品价格由市场决定。但农产品大豆、豆粕与其他火热商品相比，就显得冷清，整个二季度，豆类市场价格几乎无像样的涨跌之势，而市场各种各样的良好预期也随着时间的推移一次又一次地让市场否定。