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91.
AIM:To explore the effect of brain ischemia injury on cell proliferation and nestin expression in cortex and subependymal zone (SEZ).METHODS:Using a local brain ischemia model(MCAO), BrdU positive cells of cortex and subependymal zone (SEZ), also nestin positive cells, were observed by immunohistochemistry.RESULTS:BrdU and nestin positive cells in SEZ of MCAO rats were obviously increased. In cortex, only nestin positive cells were observed.CONCLUSION:Neural stem cells in SEZ and cortex were activated after brain ischemia, it may be related with neural recovery after brain ischemia injury. 相似文献
92.
Glucoraphanin (4-甲基亚磺酰丁基芥子油苷 )在青花菜中含量丰富 ,其降解产物Sulforaphane具很强的抗癌活性。本试验以‘Marathon’青花菜为试材 ,将新鲜花球切成小花球后用 10 0mg/L的NaClO表面消毒 1min ,清水洗净。分别取 6 5 0g花球置于 0℃、 5℃和 10℃冷藏 ,并在 5℃下进行 5种气调处理 :1)Air,2 )Air+10 %CO2 ,3)Air+2 0 %CO2 ,4) 1%O2 ,5 ) 1%O2 +10 %CO2 。每一处理分别在处理当天、处理后 3、 6、 9、 12d取样 5 0g ,真空冷冻干燥 ,并研磨成粉末状贮藏于 4℃待用。将 0 .2g冷冻干燥样品在沸水中煮 10min ,35 0 0g离心 5m… 相似文献
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AIM: To investigate the application of atomic force microscope (AFM) in clinic diagnosis. METHODS: Topographic images and some parameters of large range field and microstructures of erythrocytes in the blood of normal subjects, lung cancer and myelodisplastic syndrome (MDS) patients were examined by atomic force microscope. RESULTS: Many clear topographic images of many erythrocytes, single erythrocyte, and microstructure of erythrocyte membrane surface were obtained. Many erythrocytes in lung cancer patients were found to change into echinocytes. One erythrocyte had 10-20 protuberances, most of which, with a mean width of 589. 0 nm and a length of 646. 7 nm, were on the edge of cells. The protuberances on the center of echinocytes are lodged and embedded. The erythrocytes of MDS patients were biconcave in shape. Many apertures with different diameters of tens to hundreds nanometer appeared on the surface of cell membrane. CONCLUSIONS: AFM can be widely applied in clinic pathological inspection, including quantification of cells, obtainment and comparison of many parameters (such as diameter, thickness, volume, surface, surface area/volume ratio), observation of topograph of single cell, and observation and comparison of membrane surface microstructure of cells, and so on. 相似文献
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AIM:To study the effect of TGF-β1 and TNF-α antisense PS-ODNS on ex vivo expansion of hematopoietic stem/progenitor cells (HSPC). METHODS:CD34+cells were purified from fresh umbilical cord blood by immunomagnetic beads, and mononuclear cells were purified from bone marrow by Ficoll-hypaque. The effects of TGF-β1 and /or TNF-α antisense PS-ODNS on ex vivo expansion of CD34+ cells、CFU-GEMM、CFU-GM、CFU-E and BFU-E were detected by using liquid and semi-solid culture systems.RESULTS:TGF-β1 antisense PS-ODNS cooperated with cytokines increased the number of CD34+ cells, CFU-GEMM, CFU-GM, CFU-E and BFU-E, which was as 4, 2.6, 2.7, 1.8, 2.1 times as that of the control (the cytokines combination), respectively. TNF-α antisense PS-ODNS cooperated with cytokines respectively increased the number of CD34+ cells, CFU-GEMM, CFU-GM, CFU-E and BFU-E by 4, 2.9, 2.6, 1.7, 1.8 times as that of the control. The above two antisense PS-ODNS cooperated with cytokines could respectively increased the number of CD34+ cells, CFU-GEMM, CFU-GM, CFU-E and BFU-E by 5.3, 2.1, 2.7, 1.9, 1.8 times as that of the control.CONCLUSION:Inhibition of endogenous TGF-β1 and TNF-α by antisense PS-ODNS will be one of the effective methods to expand HSPC ex vivo. 相似文献
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