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81.
Nobuya Tashiro Ayako Urakawa Kazuyuki Shoji Yoichi Matsuo Toyozo Sato 《Journal of General Plant Pathology》2018,84(5):381-385
In early January 2015–2017, anthracnose was detected on Satsuma mandarin orange (SMO) (Citrus unshiu) fruits kept in farmers’ storage rooms in Saga Prefecture, Japan. Three single-spore isolates from rotten fruits reproduced the postharvest anthracnose symptoms in wound-inoculated SMO fruits and were re-isolated from lesions. The isolates were identified as Colletotrichum fioriniae based on conidial morphology, culture characteristics, rDNA-ITS, and beta-tubulin-2 gene sequences. This is the first report of postharvest anthracnose on SMO caused by C. fioriniae. 相似文献
82.
Keiji Takami Hiroyuki Okubo Shuichi Yamasaki Minoru Takeshita Yoichi Takanami 《Journal of General Plant Pathology》2006,72(6):391-392
Momordica charantia L. plants systemically infected with Cucumber mosaic virus (CMV) were found in Oita Prefecture. The virus isolated from the host plant was characterized by biological, serological,
and molecular biological methods. The purified virus was used to mechanically inoculate the host and produced green mottle,
green mosaic, and/or chlorotic spots in the noninoculated upper leaves of the host. The virus was identified as an isolate
of CMV containing genomic RNA3 derived from subgroup IA by several lines of evidence based on electron microscopy, serological
detection, host range, symptoms, and the entire nucleotide sequence of RNA3. 相似文献
83.
A strain of Cucumber mosaic virus (CMV-D8) systemically infects Japanese radish (Raphanus sativus), but the Y strain of CMV (CMV-Y) only infects the inoculated leaves. Both of these strains cause severe systemic mosaic on
the plants after dual infection with Turnip mosaic virus (TuMV). Synergistic interactions on long-distance transport of CMV-Y and CMV-D8 with TuMV were analyzed using an immunobinding
assay. Direct tissue blots probed with either anti-CMV-Y or anti-TuMV antiserum clearly showed that CMV-Y efficiently spread
and accumulated in the tissues of noninoculated upper leaves and roots when co-inoculated with TuMV, and that long-distance
movement of CMV-D8 was enhanced by the presence of TuMV.
Received 16 September 1999/ Accepted in revised form 5 February 2000 相似文献
84.
Mikiko FUKUDA Mayuko INOUE Daisuke MURAMATSU Hitoshi MIYACHI Yoichi SHINKAI 《The Journal of reproduction and development》2016,62(1):121-125
Production of knockout mice using targeted embryonic stem cells (ESCs) is a powerful approach for
investigating the function of specific genes in vivo. Although the protocol for gene
targeting via homologous recombination (HR) in ESCs is already well established, the targeting efficiency
varies at different target loci and is sometimes too low. It is known that knockdown of the Bloom syndrome
gene, BLM, enhances HR-mediated gene targeting efficiencies in various cell lines. However,
it has not yet been investigated whether this approach in ESCs is applicable for successful knockout mouse
production. Therefore, we attempted to answer this question. Consistent with previous reports,
Blm knockdown enhanced gene targeting efficiencies for three gene loci that we examined by
2.3–4.1-fold. Furthermore, the targeted ESC clones generated good chimeras and were successful in germline
transmission. These data suggest that Blm knockdown provides a general benefit for efficient
ESC-based and HR-mediated knockout mouse production. 相似文献
85.
86.
87.
Aoshima H Takeda K Okita Y Hossain SJ Koda H Kiso Y 《Journal of agricultural and food chemistry》2006,54(7):2514-2519
Beer induced the response of the ionotropic gamma-aminobutyric acid receptors (GABA(A) receptors) expressed in Xenopus oocytes, indicating the presence of gamma-aminobutyric acid (GABA)-like activity. Furthermore, the pentane extract of the beer, hop (Humulus lupulus L.) oil, and myrcenol potentiated the GABA(A) receptor response elicited by GABA. The GABA(A) receptor responses were also potentiated by the addition of aliphatic esters, most of which are reported to be present in beer flavor. Aliphatic esters showed the tendency to decrease in the potentiation of the GABA(A) receptor response with an increase in their carbon chain length. When myrcenol was injected to mice prior to intraperitoneal administration of pentobarbital, the pentobarbital-induced sleeping time of mice increased additionally. Therefore, the beer contained not only GABA-like activity but also the modulator(s) of the GABA(A) receptor response. 相似文献
88.
A sharp peak of the zero-temperature penetration depth at optimal composition in BaFe2(As(1-x)P(x))2
Hashimoto K Cho K Shibauchi T Kasahara S Mizukami Y Katsumata R Tsuruhara Y Terashima T Ikeda H Tanatar MA Kitano H Salovich N Giannetta RW Walmsley P Carrington A Prozorov R Matsuda Y 《Science (New York, N.Y.)》2012,336(6088):1554-1557
In a superconductor, the ratio of the carrier density, n, to its effective mass, m*, is a fundamental property directly reflecting the length scale of the superfluid flow, the London penetration depth, λ(L). In two-dimensional systems, this ratio n/m* (~1/λ(L)(2)) determines the effective Fermi temperature, T(F). We report a sharp peak in the x-dependence of λ(L) at zero temperature in clean samples of BaFe(2)(As(1)(-x)P(x))(2) at the optimum composition x = 0.30, where the superconducting transition temperature T(c) reaches a maximum of 30 kelvin. This structure may arise from quantum fluctuations associated with a quantum critical point. The ratio of T(c)/T(F) at x = 0.30 is enhanced, implying a possible crossover toward the Bose-Einstein condensate limit driven by quantum criticality. 相似文献
89.
Molecular sexing of Japanese cormorants used for traditional fishing on the Nagara River in Gifu City 总被引:2,自引:0,他引:2
Miho INOUE-MURAYAMA Yuko UEDA Tetsuji YAMASHITA Chizuko NISHIDA-UMEHARA Yoichi MATSUDA Toshiaki MASEGI Shin'ichi ITO 《Animal Science Journal》2002,73(5):417-420
The Japanese cormorants used in traditional fishing in Japan are wild derived and their sex cannot be determined from their appearance. Applicability of molecular sex determination based on the size difference between CHD1Z and CHD1W introns was confirmed in male and female Japanese cormorants whose sexes had been ascertained by pathological autopsy. All of 21 birds of unknown sex reared by a cormorant fishing master were identified as males. The molecular sexing method will provide valuable information on sex differences of wild Japanese cormorants, including tameness, trainability, behavior and fishing capability, as well as for future trials involving artificial reproduction. 相似文献
90.
Yoichi Miyake Shingo Kimura Tomohiko Kawamura Takashi Kitagawa Motoyuki Hara Hiroshi Hoshikawa 《Fisheries Science》2010,76(4):561-570
Most juveniles of Haliotis discus hannai have been found to be descendants of wild individuals, although most adults were artificially produced (released) individuals
as a result of restocking inside the refugium located near the head of Oshoro Bay, Hokkaido, Japan. To estimate the larval
supply from released and wild individuals into the refugium and to compare the suitability of locations as larval sources,
we simulated larval dispersal using a coupled hydrodynamic and particle-tracking model. The simulation results indicated that
more larvae may be supplied from the wild adults inside the bay to the refugium than from the released adults. These results
are consistent with the observed high abundance of wild juveniles in the refugium. Most larvae from the refugium were predicted
to disperse out of the bay. We found that larval retention in the bay was at least one order of magnitude higher than that
in the refugium. Thus, it may be more effective in terms of self-replenishment and reproduction if the refugium were to be
expanded to the bay scale. There were only minor differences among the compared sites at the head of the bay in terms of their
suitability as larval sources. Consequently, the establishment of new refugia in this area could be expected to provide an
effectiveness equal to that of the current refugium. 相似文献