Model establishment and biological behaviour observation of mouse blastocyst co-cultured with hepatocarcinoma cell lines with differently invasive and metastatic potential in vitro

AIM: To explore interaction and biological behaviour changes of two kinds of cells-blastocysts and hepatocarcinoma cells in the same microenvironment. METHODS:The models of mouse blastocysts co-cultured with human hepatocarcinoma cell lines were established, then biological behaviours and mutual effects of the two kinds of cells in co-culture system were observed. RESULTS: Compared with control group, hepatocarcinoma cells with differently invasive and metastatic potential significantly enhanced the rates of blastocyst hatchment , attachment and outgrowth(P＜0.05). There was no significant difference in those among hepatocarcinoma cells co-cultured groups (P＞0.05). The blastocyst hatched and attached to hepatocarcinoma cells with differently invasive and metastatic potential. Then, differential trophoblasts invaded hepatocarcinoma cells. The clear-cut interfaces were gradually formed between both sides. Hepatocarcinoma cells on interface showed changes of growth direction and cell shapes and did not invade blastocysts. CONCLUSIONS: Hepatocarcinoma cells promoted blastocyst development. Blastocysts implanted and invaded hepatocarcinoma cells with differently invasive and metastatic potential in vitro, which indicate that blastocyst implantation in vitro does not relate with the kinds and differential level of interactional cells and the low selectivity maybe relate with high adaptability of early life.     

Impact of cold-season grazing and supplementary feeding on rumen metabolic parameters and micro-organisms in the ewes of Gansu Alpine Fine Wool Sheep北大核心CSCD

An experiment was conducted to scientifically identify the appropriate feed for Gansu Alpine Fine Wool ewes in order to change the animals' breeding mode, which currently can only be mated at the age of 2.5 years due to insufficient nutritional supply. The experiment selected 48 12-month-old ewes and randomly divided them into group a (Diet 1), group b (Diet 2), group c (Diet 3), and a control group with no supplementary feeding. The feed test was run for 90 days. The rumen pH of groups a and b were very significantly higher (P<0.01) or group c significantly lower (P<0.05) than control group. The rumen ammonia nitrogen, protein nitrogen and total nitrogen of groups a, b and c were all higher than control group (P<0.01). The total volatile fatty acids of group a and b were all higher than the control group (P<0.01), group b significantly higher than group c (P<0.05). The molar ratio of acetic acid and propionic acid in group a was very significantly higher than control group (P<0.01), group b and c were significantly higher than control group (P<0.05). The molar ratio of butyric acid in group a was very significantly higher than group c (P<0.01). The ratio of acetic acid and propionic acid in group b was significantly lower than control group (P<0.05). The number of several rumen fiber-degrading bacteria and protozoa in groups a, b and c were very significantly (P<0.01) or significantly (P<0.05) higher than the control. In conclusion, this study shows that supplementary feeding can positively affect the rumen metabolic parameters and the number of several rumen microorganisms in Gansu Alpine Fine Wool ewes. In general, the effects of Diet 1 were the strongest, while Diet 2 also produced notable improvements. © 2018, Editorial Office of Acta Prataculturae Sinica. All rights reserved.     

如何在和谐社会中培养优秀的音乐教育人才

未来社会需要高素质的、德才兼备的、具有宽广知识、一专多能的音乐教育人才.在提高专业技能知识的同时,培养学生综合素质,是当前和谐社会中培养高素质人才的关键.     

皖西南稻谷产区烘干机应用调查报告

根据南方高水分晚稻形成的原因、稻谷干燥的现状,提出特别是使用燃煤、稻壳炉的小型移动式低温循环烘干机适合于南方的稻谷干燥,以及低温循环烘干机目前存在问题的解决措施.     

上海水牛生理生化指标的测定和分析

为研究上海水牛生理生化指标含量,选取精神良好、健康的上海水牛犊牛(1岁内)、青年牛(1-2岁)、成年牛(3岁以上),测定了生理指标、血液生理生化指标含量。结果显示,上海水牛的体重随月龄的增加显著增加(P＜0.05),而呼吸频率、心率无显著差异(P＞0.05)。在检测的22项血液生理指标含量中,有10项指标(平均红细胞体积(MCV)、平均红细胞血红蛋白含量(MCH)、平均红细胞血红蛋白浓度(MCHC)、红细胞分布宽度(RDW)、淋巴细胞百分比(%LYM)、单核细胞百分比(%MONO)、中性粒细胞百分比(%NEU)、嗜酸性粒细胞百分比(%EOS)、淋巴细胞(LYM)、单核细胞(MONO))含量差异显著(P＜0.05),其余12项指标(红细胞(RBC)、红细胞比容(HCT)、血红蛋白(HGB)、网织红细胞百分比(%RETIC)、网织红细胞(RETIC)、白细胞(WBC)、嗜碱性粒细胞百分比(%BASO)、中性粒细胞(NEU)、嗜酸性粒细胞(EOS)、嗜碱性粒细胞(BASO)、血小板(PLT)、血小板压积(PCT))含量差异不显著(P＞0.05)。在检测的18项血液生化指标含量中,有9项指标(血糖(GLU)、肌酐(CREA)、磷离子(PHOS)、钙离子(Ca)、总蛋白(TP)、球蛋白(GLOB)、丙氨酸转氨酶(ALT)、胆固醇(CHOL)、胰淀粉酶(AMYL))含量差异显著(P＜0.05),其余9项指标(尿素(UREA)、血尿素氮/肌酐比(BUN/CREA)、白蛋白(ALB)、白蛋白/球蛋白比(ALB/GLOB)、碱性磷酸酶(ALKP)、谷氨酰转移酶(GGT)、总胆红素(TBIL)、甘油三酯(TRIG)、脂肪酶(LIPA))含量差异不显著(P＞0.05)。该研究可为上海水牛的选种选育、生长发育评估、营养状况评估、疾病诊断等提供参考。     

城市夜间形象初探

从城市规划和建筑学的角度研究城市夜间形象;结合形态美学、行为心理学、环境心理学等学科进行研究,将安全感、视觉舒适性、视觉趣味、活动激发性以及可识别性作为夜间形象评价指标并分别进行具体地分析。     

土壤和沉积物中PAHs环境行为研究进展

PAHs作为持久性有机污染物对人类健康和生态安全构成威胁,研究PAHs环境行为对明确我国PAHs污染现状和防治PAHs污染具有重要意义.文章综述国内外对城市、河流和山区土壤和沉积物中PAHs区域分布和纵向分布研究,列举常见PAHs来源和来源判断方法.归纳PAHs释放和迁移两种重要环境行为,针对释放行为详细讨论沉积物再悬...     

稻田免耕稻草全程覆盖秋马铃薯的效果及技术

宜昌市于2007秋在枝江市示范种植稻田免耕稻草全程覆盖秋马铃薯36.67 hm2举办核心示范样板3.33 hm2.平均每公顷产鲜薯19695 kg、纯收入19997.4元,整个示范区平均每公顷纯收入15000元以上,其主要栽培要点是备好种薯、适时播种、施足底肥、保证密度、盖好稻草、精心田管、搞好化调.     

四种禾草补播"黑土型"退化草地效果的初步观测

在玉树藏族自治州囊谦县东坝乡“黑土型”退化草地上补种了披碱草(ElymusnutansGriseb)、老芒麦(E.sibiricusL)、草地早熟禾(PoapratensisL)和中华羊茅(Festucasinensiskeng)四种牧草,补种当年均处于营养生长,不能完成生育期,第二年平均每公顷产干草6839kg~9407kg,适应性强,越冬性良好,是改良“黑土型”退化草地的优良牧草。     

漆酶活化法竹材刨花板的制造工艺

分别采用正交试验和单因素试验方法研究漆酶活化法制造竹材刨花板的酶处理工艺和热压工艺,结果表明:竹材刨花漆酶处理优化工艺参数为酶用量30 U/g、反应体系pH值4、处理时间2 h、处理温度60 ℃;当板材密度为0.70 g*cm-3、厚度为10 mm时,热压优化工艺参数为热压压力4.0 MPa、热压温度200 ℃、板坯芯层温度140 ℃、热压时间17.5 min.