中澳合作开展“中国西北部省区土地资源可持续利用”研究项目取得阶段性成果

7月10日，由国家林业局经济发展研究中心与澳大利亚国立大学共同举办的“中国西北部省区土地资源可持续利用”研究项目成果研讨会在北京隆重举行。国家林业局副局长张建龙、澳大利亚驻中国大使馆农业参赞Mr．Hudson、ACIAR项目官员Trennett博士到会祝贺，澳大利亚国立大学Bennett教授、国家林业局经济发展研究中心主任张蕾、国家林业局经济发展研究中心副主任戴广翠等项目组成员先后就《项目研究目标及大纲》、《项目实施、许可及政策意义》、《退耕还林综合效益与成本分析及政策建议》、     

Isolation,Identification and Drug Sensitivity Test of a Strain of Aeromonas veronii from Chinemys reevesii

To determine the cause of death of Chinemys reevesii in a pet shop in Changli county, Hebei province, a dominant strain CLW-1 was isolated and purified from the diseased Chinemys reevesii. It was analyzed and identified through anatomical observation, bacterial isolation and culture, Gram staining and microscopic examination, biochemical identification and 16S rDNA gene sequencing, the drug resistance of the strain was detected through drug sensitivity test, and its pathogenicity was detected through zebrafish inoculation test and virulence gene experiment. The results showed that the bacteria formed moist, smooth surface, gray colony with β hemolysis on blood agar medium, and grew smooth and moist yellow colony on RS agar medium; Gram staining and microscopic examination showed negative coccidiosis; Homology analysis of 16S rDNA gene sequence showed that the isolated strain CLW-1 belongs to the same group with Aeromonas veronii, and the homology is above 99%; Inoculation test results of zebrafish showed that the strain had high pathogenicity, and its LD₅₀ was 1.26×10⁶ CFU; The virulence gene amplification results showed that the isolated strain CLW-1 had virulence genes of aerA, Exu, Lip and Ser; Drug sensitivity test results showed that the isolated strain CLW-1 was sensitive to piperacillin, cefazolin and enrofloxacin, etc; Susceptibility to furazolidone, oxacillin and erythromycin, etc; Resistance to midecamycin, clindamycin and vancomycin, etc. According to the test results, the isolated strain CLW-1 is confirmed to be Aeromonas veronii, which carries virulence genes and has high pathogenicity, it has great therapeutic effect by applying corresponding antibiotics, thus laying a foundation for the prevention and treatment of Aeromonas veronii.     

人结核病病原菌型的PCR指印分析

以结核分枝杆菌特异插入序列ＩＳ６１１０两端序列为模板,设计一对外向ＰＣＲ引物进行ＰＣＲ扩增,从而建立一种结核分枝杆菌的快速分子生物学分型技术,该试验的基础是利用ＩＳ６１１０在结核分枝杆菌染色体ＤＮＡ中反复重复且ＩＳ６１１０序列之间相距较近,经ＰＣＲ扩增呈现多条带型构成ＤＮＡ指印。采用ＰＣＲ指印技术,对人结核病患者痰标本中的结核分枝杆菌进行分型,５７份痰标本呈现７种ＰＣＲ指印。该ＰＣＲ分型技术对结核     

放牧强度对羊草草甸草原植物器官及群落氮磷化学计量的影响

[目的]以地上植物茎、叶以及群落为研究对象,探究不同放牧强度对植物器官以及群落碳、氮、磷含量及化学计量特征的影响,为深入解析放牧干扰下草地生态系统退化过程,促进草地放牧适应性管理提供科学依据.[方法]基于呼伦贝尔羊草草甸草原长期控制放牧试验平台,在6种不同放牧强度(不放牧G0.00、较轻度放牧G0.23、轻度放牧G0....     

珠江三角洲城市群城市森林体系的框架构建

在现状调查分析的基础上,提出建设珠江三角洲城市森林生态体系的框架、战略思路和政策建议。       

Ca2+对过量Fe2+胁迫下水稻保护酶活性及膜脂过氧化的影响

采用水培法研究了不同浓度Ca2+在过量Fe2+胁迫下对水稻生长、保护酶(SOD、POD、CAT)活性及脂质过氧化的影响.结果表明,Fe2+供应正常时,100～300 mg kg-1Ca2+的水培液能不同程度地促进水稻生长,提高SOD、POD活性,而对CAT的影响不大,高钙(400 mg kg-1)供应则会加剧脂质过氧化.在过量Fe2+胁迫下,100～300 mg kg-1Ca2+能较好地促     

河南省农业资源利用探析

为充分合理利用河南省的农业资源,笔者探析了河南省农业资源利用存在的问题,调查和研究资料表明：河南省人口的持续增长为农业资源利用带来很大压力,耕地面积不断减少、质量下降,多数耕地生产力较低;并不丰富的水资源,近几年污染严重、农业用水浪费普遍,用水效率急待提高;气候资源较为丰富,但仍未能充分利用,气象灾害危害较大;农作物种类多,农业生物资源比较丰富,部分地区的小麦、玉米等粮食作物产量高,但生产技术条件有待提高。最后针对存在的问题,提出解决对策。     

甘蓝田节肢动物群落结构研究

在田间系统调查基础上研究了合肥郊区甘蓝田节肢动物群落结构。调查共记录节肢动物21种,其中害虫10种,天敌11种。害虫主要优势种为菜青虫和菜蚜为优势种;捕食性天敌主要优势种为中华跃蛛、草间小黑蛛。害虫优势种菜青虫主要分布于甘蓝心叶和内层叶反面,而菜蚜分布较广。捕食性天敌优势种中华跃蛛、草间小黑蛛主要分布于甘蓝外层叶反面。群落多样性在时间过程中较稳定,影响群落多样性的主要因素是群落均匀度。     

Zokor activity promotes soil water infiltration in the Mu Us sandy land of northern Shaanxi, China

Zokors are common subterranean rodents that inhabit agricultural fields, shrublands, and grasslands in the arid and semi-arid regions of China. Zokor burrowing activities can alter soil structure and affect soil hydrological processes; however, there are few studies regarding their effects on soil preferential flow in the Mu Us sandy land. An evaluation of the effects of zokor disturbance on their habitat and soil water is important for understanding the ecological role of zokors in the soil eco...     

单精子显微注射技术的研究及其前景展望

单精子显微注射（Intracytoplasmic sperm injection,ICSI）技术作为辅助受精的一种手段,将体外受精研究和胚胎的显微操作技术结合起来,比以往对精子质量的要求大大降低,使其无论在畜牧业生产实践还是在哺乳动物的生殖生理基础研究中都有着十分重要的意义。本文针对ICSI技术研究概况、影响因素、应用前景及其存在问题进行简要介绍,为ICSI技术的应用提供参考。