基于转录组的疏花软紫草低拷贝核基因引物开发

基于高通量的转录组测序,为大量并快速的开发分子标记提供契机。本研究对紫草科植物疏花软紫草进行转录组测序,共得到92042086条reads,从疏花软紫草158446个Unigene中搜索到332个低拷贝核基因。为进行多态性验证,从中随机挑选30个设计引物,并对3个疏花软紫草种群共9个个体进行PCR扩增,结果发现其中16对引物能够扩增出单一稳定的目的条带,从中随机挑选7个片段进行群体测序并计算DNA多态性。结果表明,7对引物的多态性位点平均值为657,单倍型平均值为514,单倍型多态性 （HD） 在0389~0944之间,核苷酸多态性 （Pi） 为000148~001723。通过转录组测序技术开发的低拷贝核基因有较高的多态性,且可以有效地运用于群体遗传学和谱系地理学研究中。另外,这些低拷贝核基因能够被运用于软紫草属植物系统发育重建以及物种形成等研究工作中。     

机器人足球比赛防守与进攻队形转换的研究

随着机器人足球的发展,机器人防守和进攻的队形对于比赛显得尤为重要。为了加强机器人足球比赛中的防守和进攻,文章通过分析足球在不同区域时防守队形的选择、防守与进攻的队形转换、以及在极端情况下剩余机器人的有效利用等几个问题,研究和设计机器人防守和进攻队形转换的算法和策略。机器人队形的选择采用人性化的方法,大大提高了队形的形成速度,在极端状态下也做出相应的策略来加强进攻。通过实验表明,该策略增加了防守和进攻的效率,提高了进球的几率。     

Anatid herpesvirus 1 CH virulent strain induces syncytium and apoptosis in duck embryo fibroblast cultures

Anatid herpesvirus 1 (AHV-1) CH virulent strain was first isolated from an infected duck and it was found that this virus strain could induce cytopathic effect (CPE) in duck embryo fibroblast (DEF). Following AHV-1 infection, DEF showed morphological changes such as cell rounding, improved refractivity and detachment from the culture surface. However, its pathological characteristics were not adequately known. Related studies were performed and the results showed that syncytium formation could be observed as the other type of CPE in AHV-1 infection. Hematoxylin-eosin staining and 4’, 6-diamidino-2-phenylindole (DAPI) staining of infected DEF were each used to visualize the shape and distribution of chromatin within nuclei and nuclear fragmentation was observed. Chromatin condensation and margination, as well as formation of apoptotic bodies were observed by transmission electron microscopy (TEM). DNA ladder formation was detected in AHV-1 infected cells and apoptosis of the infected DEF was also detected by flow cytometry analysis of Annexin V-FITC/PI staining method. Therefore, it was suggested that AHV-1 virulent strain can induce syncytium and apoptosis in DEF. Syncytium formation and apoptosis observed in this study may contribute to the elucidation of AHV-1 pathogenesis.     

水稻株型性状的配合力分析

将１０个恢复系,５个不育系按不完全双列杂交组配,考察其Ｆ１代株型性状的配合力。结果表明,剑叶长,宽,角度,株高４个性状的遗传主要受加性效应的影响,分蘖角度受加性效应和非加性效应的影响,但主要受非加性效应的影响。不同材料的一般配合力,特殊配合力效应及其变异程度存在着差异。     

藤编原料植物青藤的扦插繁殖试验初报

本试验开展了青藤扦插繁殖试验,通过扦插季节、植物生长调节剂、遮阳对比试验,总结出了青藤的扦插繁殖技术,既可以解决藤编原料问题,同时又能达到保护野生植物资源的目的。     

锦屏县毛竹现状分析与产业发展对策

毛竹产业的发展必须以市场为导向,以增加农民收入和促进山区农村经济发展为目标,创新发展方式,加大扶持力度,按照规模上求大、品种上求新、管理上求精的要求,坚持扩大面积与提高种植质量并重,强化科技支持与产业经营体系建设,把毛竹子产业培育成山区经济发展的重要支柱产业。     

Greenhouse gas emissions in response to straw incorporation,water management and their interaction in a paddy field in subtropical central China

A field experiment was conducted to study the effects of combination of straw incorporation and water management on fluxes of CH₄, N₂O and soil heterotrophic respiration (Rh) in a paddy field in subtropical central China by using a static opaque chamber/gas chromatography method. Four treatments were set up: two rice straw incorporation rates at 0 (S1) and 6 (S2) t ha^?1 combined with two water managements of intermittent irrigation (W1, with mid-season drainage) and continuous flooding (W2, without mid-season drainage). The cumulative seasonal CH₄ emissions for the treatments of S1W2, S2W1 and S2W2 increased significantly by 1.84, 5.47 and 6.63 times, respectively, while seasonal N₂O emissions decreased by 0.67, 0.29 and 1.21 times, respectively, as compared to S1W1 treatment. The significant increase in the cumulative Rh for the treatments S1W1, S2W1 and S2W2 were 0.54, 1.35 and 0.52 times, respectively, in comparison with S1W2. On a seasonal basis, both the CO₂-equivalents (CO₂e) and yield-scaled CO₂e (GHGI) of CH₄ and N₂O emissions increased with straw incorporation and continuous flooding, following the order: S2W2>S2W1>S1W2>S1W1. Thus, the practices of in season straw incorporation should be discouraged, while mid-season drainage is recommended in paddy rice production from a point view of reducing greenhouse gas emissions.     

胶合板剪切强度试件的力学分析

通过对胶合板剪切强度试件的力学分析,表明试验机的夹头应采取上下可移动、左右固定法。在测试时,应注意取样方法,试验用水、加荷速度、计算公式和试验的判定。     

雷琼牛肌肉生长抑制素基因序列研究

通过PCR方法对18头雷琼牛的MSTN基因进行扩增、测序.编码区序列分析结果表明:雷琼牛MSTN基因比外域瘤牛存在较丰富的多态性,共发现4个突变位点(2个转换,2个颠换),定义了6种单倍型,群体单倍型多样性较高(0.810±0.057);分析结果表明:MSTN基因的相关突变的发生可能早于Bos属瘤牛、普通牛、牦牛的分化;在瘤牛种内,我国南方的瘤牛可能是更原始的种群.