Immobilization of Fe chelators on sepharose gel and its effect on their chemical properties

Iron chelates are usually costly and easily leached beyond the root zone. This creates a need to frequently replenish the rhizosphere with chelated Fe and might contaminate groundwater with organic compounds and metals. The development of a slow-release Fe fertilizer that will efficiently supply Fe to plants while exhibiting high resistance toward leaching and/or degradation in the rhizosphere has been the focus of this study. Desferrioxamine B (DFOB) and ethylenediaminebis(o-hydroxyphenylacetic acid) (EDDHA) were immobilized on Sepharose. (13)C NMR and FTIR measurements confirmed that coupling of DFOB to the gel did not appear to influence its ability to chelate Fe(3+) or its binding nature. Isotherms for the immobilized ligands were determined in the presence of 1 mM HEDTA, at 25 degrees C and at an ionic strength of 0.1 M. The isotherms showed a high affinity of Fe(3+) to the ligands and binding up to saturation level throughout the pH range examined (4.0-9.0). The K(app) values for the immobilized Fe chelates were determined using a modified Scatchard model and found to be lower than the soluble ones. This decrease in K(app) might facilitate Fe uptake from these chelates by plants.     

Complexes of Cu–Polysaccharide of a Marine Red Microalga Produce Spikes with Antimicrobial Activity

Metal–polysaccharides have recently raised significant interest due to their multifunctional bioactivities. The antimicrobial activity of a complex of Cu₂O with the sulfated polysaccharide (PS) of the marine red microalga Porphyridium sp. was previously attributed to spikes formed on the complex surface (roughness). This hypothesis was further examined here using other Cu–PS complexes (i.e., monovalent-Cu₂O, CuCl and divalent-CuO, CuCl₂). The nanostructure parameters of the monovalent complexes, namely, longer spikes (1000 nm) and greater density (2000–5000 spikes/µm²) were found to be related to the superior inhibition of microbial growth and viability and biofilm formation. When Escherichia coli TV1061, used as a bioluminescent test organism, was exposed to the monovalent Cu–PS complexes, enhanced bioluminescence accumulation was observed, probably due to membrane perforation by the spikes on the surface of the complexes and consequent cytoplasmic leakage. In addition, differences were found in the surface chemistry of the monovalent and divalent Cu–PS complexes, with the monovalent Cu–PS complexes exhibiting greater stability (ζ-potential, FTIR spectra, and leaching out), which could be related to spike formation. This study thus supports our hypothesis that the spikes protruding from the monovalent Cu–PS surfaces, as characterized by their aspect ratio, are responsible for the antimicrobial and antibiofilm activities of the complexes.     

Epidemiological investigations of outbreaks of bovine ephemeral fever in Israel

In two epidemics of bovine ephemeral fever (BEF) in Israel, one in 1990 and one in 1999, the virus was probably carried by vectors transported by air currents across the Rift Valley and through the Red Sea trough. The disease broke out under optimal ecological conditions among vulnerable cattle populations and spread rapidly; it developed in the spring and summer and ended soon after the daily average ambient temperature fell below 16 degrees C in late autumn. The proportion of herds affected reached 78.4 and 97.7 per cent in 1990 and 1999, respectively. The highest rates of incidence, morbidity and mortality were recorded in dairy cattle herds in the Jordan Valley, the initial focus of the outbreaks, with a morbidity of 20 and 38.6 per cent in 1990 and 1999, respectively, and mortality among the affected animals of 2 and 8.6 per cent in 1990 and 1999, respectively. In 1991, the disease recurred sporadically in the central and southern regions of Israel in only three herds, but in 2000 the disease returned on an epidemic scale, and 85 per cent of herds were affected, with morbidity and mortality rates of 4-3 and 0-3 per cent, respectively. In the 1999 epidemic, the morbidity rate decreased from 38-6 per cent on average in the Jordan Valley to 12.8 per cent in the inner valleys and 5.3 per cent on the Mediterranean coastal plain, but the mortality rate increased from 8-6 per cent in the Jordan Valley to 14-3 per cent in the inner valleys, and to 28 per cent on the Mediterranean coastal plain, where the outbreak declined. An average of 2-7 per cent of the animals experienced a second attack of the disease two to six weeks later. The epidemic in 2000 was milder and shorter than that in 1999. All the cattle affected in both outbreaks were more than three months old. The vector(s) is not known for certain but the available evidence indicates that mosquitoes, and not Culicoides species, are the natural vectors of BEF virus in Israel.     

Study of apparent hypersensitivity to Culicoides species in sheep in Israel

Between 1983 and 1997, a low incidence of seasonal allergic dermatitis was diagnosed in sheep in Israel aged eight months to eight years, in 10 flocks of a variety of breeds, but not the local Awassi breed. The 40 affected animals reacted with scratching, biting and vigorous stamping of the feet from April to November each year. The principal skin lesions were hyperkeratosis, spongiosis and infiltrations of eosinophils and mononuclear cells, typical of an allergic dermatitis which was probably a hypersensitivity reaction to biting insects. The epidemiological findings in relation to the area of Israel affected, the breeds of sheep and the distribution of the lesions suggested that the sheep's hypersensitivity was due to the bites of several species of Culicoides.     

The digestibility of nutrient sources for common carp, Cyprinus carpio Linnaeus

The apparent digestibility of protein, fats, carbohydrate and energy within three feed ingredients (wheat, barley and corn) for common carp, Cyprinus carpio Linnaeus, was studied, using chromic oxide as a dietary marker. Each experimental diet consisted of a mixture of the test ingredient and 50% basal diet (containing 50% soybean meal, 35% wheat meal, 10% soybean oil, plus 5% vitamins, egg yolk, guar and chromic oxide). It was found that in wheat meal the protein had an apparent digestibility of 92%, and the lipid an apparent digestibility of 80%. The apparent digestibility of corn was 81% for protein and 90% for lipid, while for barley the figures were 73% and 67%, respectively. For apparent digestible energy, the figures were 12.39 kJ g^?1 for wheat, 6.69 kJ g^?1 for barley and 9.32 kJ g^?1 for corn.     

Energy cost of cows' grazing activity: Use of the heart rate method and the Global Positioning System for direct field estimation

This study with grazing beef cows on the range was designed to explore the possibility of determining incremental energy expenditure (EE) in standing, traveling, and grazing relative to that in lying down, by means of continuous monitoring of EE, location, and activity by the heart-rate method, with Global Positioning System (GPS) collars, and by motion sensors in the GPS collars, respectively. Cows were observed on Mediterranean foothill rangeland covered with herbaceous vegetation through 4 seasons of the year. There were 2 stocking rate treatments, and 14 statistical models were evaluated, including one that was a stepwise model. Total daily EE (TEE) was affected by many interdependent factors apart from activity, including season, stocking rate, herbage quality, standing biomass, and reproductive state of the cow. Each model included all activity variables, plus some of the other factors. Across seasons and treatments TEE, in kJ/(kg of BW(0.75) . d), ranged from 469 in densely stocked, nonlactating cows in June to 1,092 in sparsely stocked, lactating cows in April. The cows' daily vertical and horizontal movements ranged from 75 to 174 m and from 1.5 to 4.2 km, respectively. Within a day, time spent traveling (without grazing) ranged from 0 to 32 min, and grazing time ranged from 4.4 to 12.1 h. Cows spent less time grazing (P < 0.001) in the summer, when herbage quality was low, than in winter and spring. Relative to the baseline EE while lying down, the daily increment incurred by grazing ranged from 13 to 48 kJ/(kg of BW(0.75) . d), and that incurred by grazing, standing, and traveling combined ranged from 38 to 74 kJ/(kg of BW(0.75) . d) or 5.8 to 11.4% of TEE. In conclusion, the estimates of activity costs yielded by 11 of the models were similar to one another, whereas those yielded by the stepwise model and the remaining 2 models were 20% smaller. The cost of grazing activity was estimated to be 6.14 J/(kg of BW(0.75) . m), and that of locomotion during grazing was 6.07 J/(kg of BW(0.75) . m), which agree with values obtained for animals and humans by means of a treadmill. The experimental and statistical approach tested here yielded fairly reliable estimations of energy costs of activities in grazing cows.     

Deformation and slip along the Sunda megathrust in the great 2005 Nias-Simeulue earthquake

Seismic rupture produced spectacular tectonic deformation above a 400-kilometer strip of the Sunda megathrust, offshore northern Sumatra, in March 2005. Measurements from coral microatolls and Global Positioning System stations reveal trench-parallel belts of uplift up to 3 meters high on the outer-arc islands above the rupture and a 1-meter-deep subsidence trough farther from the trench. Surface deformation reflects more than 11 meters of fault slip under the islands and a pronounced lessening of slip trenchward. A saddle in megathrust slip separates the northwestern edge of the 2005 rupture from the great 2004 Sumatra-Andaman rupture. The southeastern edge abuts a predominantly aseismic section of the megathrust near the equator.     

Leaf Rust on Aegilops speltoides Caused by a New Forma Specialis of Puccinia triticina

ABSTRACT A leaf rust attacking Aegilops speltoides in its natural habitat is reported for the first time. It was found in two locations in northern and central Israel. The two collections from A. speltoides resemble wheat leaf rust, Puccinia triticina, in most spore dimensions, in the morphology of the substomatal vesicle of the urediniospore, and in DNA content in pycniospore nuclei. Similarly to P. triticina isolates from wheat, isolates taken from A. speltoides are compatible with Thalictrum speciosissimum as an aecial host and they are crossed easily with wheat leaf rust isolates. However, isolates from A. speltoides differ from wheat leaf rust in their telial host range. They are avirulent to cultivated wheat cultivars, but attack hundreds of A. speltoides accessions that were immune to wheat leaf rust. This distinct host preference justifies delineation of the newly found leaf rust as a forma specialis (f. sp. speltoides) within P. triticina.     

Newly emerged nulliparous Culicoides imicola Kieffer (Diptera: Ceratopogonidae) with pigmented abdomen

The method of segregating nulliparous and parous females of Culicoides spp. based on the presence of burgundy-red pigment inside the abdominal wall of parous Culicoides midges, is used worldwide. Out of 320 females of Culicoides imicola trapped by emergence traps, set over an artificial breeding site for 10 and 24 days, 73 (22.8%) showed a red-pigmentation despite the fact that they were nulliparous. This finding indicated that 23% of the “parous” females that are examined for the presence of arboviruses and other pathogens or for age-grading purposes, are actually old nulliparous females, which had no chance of acquiring pathogens. This bias in parous rate distorts upward the calculation of vectorial capacity.