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11.
Hepatic glucose sensing via the CREB coactivator CRTC2 总被引:1,自引:0,他引:1
Chronic hyperglycemia contributes to the development of diabetes-associated complications. Increases in the concentration of circulating glucose activate the hexosamine biosynthetic pathway (HBP) and promote the O-glycosylation of proteins by O-glycosyl transferase (OGT). We show that OGT triggered hepatic gluconeogenesis through the O-glycosylation of the transducer of regulated cyclic adenosine monophosphate response element-binding protein (CREB) 2 (TORC2 or CRTC2). CRTC2 was O-glycosylated at sites that normally sequester CRTC2 in the cytoplasm through a phosphorylation-dependent mechanism. Decreasing amounts of O-glycosylated CRTC2 by expression of the deglycosylating enzyme O-GlcNAcase blocked effects of glucose on gluconeogenesis, demonstrating the importance of the HBP in the development of glucose intolerance. 相似文献
12.
1,3‐Dichloropropene (1,3‐D), which consists of two isomers, (Z)‐ and (E)‐1,3‐D, is considered to be a viable alternative to methyl bromide, but atmospheric emission of 1,3‐D is often associated with deterioration of air quality. To minimize environmental impacts of 1,3‐D, emission control strategies are in need of investigation. One approach to reduce 1,3‐D emissions is to accelerate its degradation by incorporating organic amendments into the soil surface. In this study, we investigated the ability of four organic amendments to enhance the rate of degradation of (Z)‐ and (E)‐1,3‐D in a sandy loam soil. Degradation of (Z)‐ and (E)‐1,3‐D was well described by first‐order kinetics, and rates of degradation for the two isomers were similar. Composted steer manure (SM) was the most reactive of the organic amendments tested. The half‐life of both the (Z)‐ and (E)‐isomers in unamended soil at 20 °C was 6.3 days; those in 5% SM‐amended soil were 1.8 and 1.9 days, respectively. At 40 °C, the half‐life of both isomers in 5% SM‐amended soil was 0.5 day. Activation energy values for amended soil at 2, 5 and 10% SM were 56.5, 53.4 and 64.5 kJ mol?1, respectively. At 20 °C, the contribution of degradation from biological mechanisms was largest in soil amended with SM, but chemical mechanisms still accounted for more than 58% of the (Z)‐ and (E)‐1,3‐D degradation. The effect of temperature and amendment rate upon degradation should be considered when describing the fate and transport of 1,3‐D isomers in soil. Use of organic soil amendments appears to be a promising method to enhance fumigant degradation and reduce volatile emissions. Published in 2001 for SCI by John Wiley & Sons, Ltd 相似文献
13.
Ergot, caused by Claviceps africana , has emerged as a serious threat to sorghum hybrid seed production worldwide. In the absence of gene-for-gene-based qualitative resistance in commercial cultivars, varieties with high pollen production that can escape ergot infection are preferred. Recent demonstration of differences in ergot susceptibility among male-sterile lines has indicated the presence of partial resistance. Using chitin-specific fluorescin-isothiocyanate-conjugated wheat germ agglutin and callose-specific aniline blue, this study investigated the process of sorghum ovary colonization by C. africana . Conidia germinated within 24 h after inoculation (a.i.); the pathogen was established in the ovary by 79 h a.i., and at least half of the ovary was converted into sphacelial tissue by 120 h a.i. Changes in fungal cell wall chitin content and strategic callose deposition in the host tissue were associated with penetration and invasion of the ovary. The rate of ovary colonization differed in three male-sterile lines that also differed in ergot susceptibility. This work demonstrates a possible histological basis for partial resistance in male-sterile sorghum lines that could lay the foundation for variety improvement through further breeding and selection. 相似文献
14.
15.
G. Singh M. Th. Van Genuchten W. F. Spencer M. M. Cliath S. R. Yates 《Water, air, and soil pollution》1996,86(1-4):137-149
Effluent concentration of chloride and two pesticides (prometon and atrazine) were measured during column displacement experiments at two water flow rates. A constant suction of approximately 1300 Pa was maintained in the packed soil columns which were positioned vertically on top of a vacuum chamber enclosing an automatic fraction collector. Measured breakthrough curves (BTC's) were analyzed in terms of two solute transport models: the standard two-parameter convection-dispersion equation (CDE), and a four-parameter two-site/two-region nonequilibrium model (TRM). Calculations obtained with the TRM model were found to be in better agreement with measured BTC's than predictions using the CDE model. Column retardation factors for prometon and artrazine calculated from equilibrium batch sorption coefficients were comparable to those estimated from the observed BTC's only when the nonequilibrium TRM model was used. 相似文献
16.
Two hundred feral cats from the inner suburbs of Sydney were examined post mortem for adult Dirofilaria immitis and circulating microfilariae, and 101 of these cats were tested for heartworm antigens by an ELISA. Only 2 cats (1%) had adult heartworms, the blood sample from another cat contained a single microfilaria. The blood of a further three cats contained small amounts of D immitis antigen. Although D immitis occurs in cats in Sydney, the prevalence is not high enough to warrant prophylactic treatment. 相似文献
17.
Stishovite, a dense phase of silica, has become widely accepted as an indicator of terrestrial impact events. Stishovite occurs at several impact structures but has not been found at volcanic sites. Solid-state silicon-29 magic-angle spinning nuclear magnetic resonance (silicon-29 MAS NMR) and X-ray diffraction of samples from the Cretaceous-Tertiary boundary layer at Raton, New Mexico, indicate that stishovite occurs in crystalline mineral grains. Stishovite was indicated by a single, sharp resonance with a chemical shift value of -191.3 ppm, characteristic of silicon in octahedral coordination, that disappeared after heating the sample at 850 degrees Celsius for 30 minutes. An X-ray diffraction pattern of HF residuals from the unheated sample displayed more than 120 peaks, most of which correspond to quartz, zircon, rutile, and anatase. Eight unambiguous weak to moderate reflections could be ascribed to d-spacings characteristic of stishovite. 相似文献
18.
Nanotechnology creates a new challenge for materials characterization because device properties now depend on size and shape as much as they depend on the traditional parameters of structure and composition. Here we show that Z-contrast tomography in the scanning transmission electron microscope has been developed to determine the complete three-dimensional size and shape of embedded structures with a resolution of approximately 1 cubic nanometer. The results from a tin/silicon quantum dot system show that the positions of the quantum dots and their size, shape, structure, and formation mechanism can be determined directly. These methods are applicable to any system, providing a unique and versatile three-dimensional visualization tool. 相似文献
19.
Johan Tidblad Vladimir Kucera Alexandre A. Mikhailov Jan Henriksen Katerina Kreislova Tim Yates Bruno Stöckle Manfred Schreiner 《Water, air, and soil pollution》2001,130(1-4):1457-1462
The main results of the International Co-operative Programme on Effects on Materials, including Historic and Cultural Monuments (ICP Materials) within the United Nations Economic Commission for Europe (UN ECE) are summarised. The 8-year field exposure programme involves 39 test sites in 12 European countries and in the United States and Canada. Dose-response functions (DRF) expressing the effect of dry and wet deposition as individual terms have been obtained for a wide range of materials including bronze, copper, weathering steel, zinc, aluminium, nickel, tin, stone materials, paint coatings and glass materials. The DRF's includes parameters that are easily available on different geographical scales and can be used for mapping areas of increased corrosion rates and for calculation of costs. 相似文献
20.
Accelerated Degradation of Methyl Isothiocyanate in Soil 总被引:1,自引:0,他引:1
Methyl isothiocyanate (MITC, CH3NCS) is the primary breakdown product of metam-sodium, and a potential replacement fumigant pesticide for methyl bromide. Methyl isothiocyanate is toxic and has a high potential for volatilization, therefore, minimizing its atmospheric emission is of the utmost importance. One method to reduce fumigant emissions is to enhance their degradation by incorporating organic amendments into the soil surface. In this study we determined the combined effect of temperature and chicken manure application rate on the degradation of MITC. The degradation of MITC was significantly accelerated by both increasing temperature and amendment rate. Differences between sterile and nonsterile degradation kinetics in unamended and organically amended soil indicate that MITC degradation is equally controlled by chemical and biological processes. The amelioration of soil with organic amendments should be further considered when designing fumigation practices that allow for reduced emissions. 相似文献