Bovine leukemia virus genotype surveillance in cattle at a slaughterhouse in Aichi Prefecture,Japan, in 2019 using polymerase chain reaction combined with restriction fragment length polymorphism

Polymerase chain reaction (PCR) combined with restriction fragment length polymorphism (RFLP) is commonly used for genotyping bovine leukemia virus (BLV) in slaughterhouses. However, unclassified BLV genotypes have been sporadically reported. To assess the current status of BLV genetic characterization in cattle, PCR-RFLP was performed on blood samples of 170 cattle (84 Japanese Black, 60 Japanese Black x Holstein, and 26 Holstein) from 17 farms (5 prefectures) at a slaughterhouse in Aichi Prefecture in 2019. A total of 65 samples (38.2%) were BLV positive, and genotype 1 was the most predominant (56/65 samples), followed by genotypes 3 (6 samples) and 5 (1 sample), and two unclassified samples. No relationship between the genotypes and breeds was observed. Sequence and phylogenetic analyses demonstrated that unclassified BLV genotypes clustered with genotype 1 sequences were, therefore, not new genotypes.     

Difference in diffusion coefficients of 14NO3 −-N and 15NO3 −-N affects the apparent fractionation factor associated with denitrification in soil

Abstract

We previously analyzed the effect of nitrate dispersion on the apparent nitrogen isotope fractionation factor associated with denitrification in soil (Kawanishi et al. 1993), and found that the dispersion effect was significant when the water flow was slow. In the previous report, we assumed that the dispersion coefficients of ¹⁴NO₃ ^? and ¹⁵NO₃ ^? were similar. However, when the water flow is slow, molecular diffusion will dominate mechanical dispersion and the above assumption may not be valid.     

A new method for nondestructive evaluation of solid wood moisture content based on dual-energy X-ray absorptiometry

This paper presents a new method to determine the moisture content of solid wood based on the principle of dual-energy X-ray absorptiometry. The study investigates the theoretical relationship between X-ray wavelength and mass attenuation coefficients of wood, water, and reference substance. In accordance with this relationship, a theoretically obtained equation is proposed to calculate the moisture content in wood. The proposed equation is compared to experimental results using small blocks of sugi wood, which showed that the change in mass attenuation coefficient of wood with X-ray tube voltage increased with increasing moisture content as expected from the theoretical equation. A regression equation for moisture content estimation was determined based on the experimental results, and the standard error of estimate in the 0–120 % dry-basis moisture content range using the regression equation was determined to be 21.9 % with the most appropriate pair of tube voltages, 15 and 40 kVp. The accuracy of the method will be improved by reducing the duration of X-ray radiography and by increasing the disparity between paired tube voltages. This method has the potential to determine moisture content of solid wood using X-ray without oven-drying or assuming oven-dry density.     

Effect of wood species on thermal flow behavior and physical properties of thermoplastic moldings

The thermoplastic flow behavior of cedar flour steamed at different temperatures in the range of 160–220°C was measured using a rheometer and compared with that of beech flour. The temperature at which the cedar flour starts to flow was approximately 70°C higher than that of beech flour, and the cedar flour exhibited low flowability. Furthermore, thermoplastic moldings were prepared from cedar and beech flours, and their physical properties were examined. Similar to the case of beech, a resin-like molding with a density of approximately 1.45?g/cm³ was obtained from the cedar flour steamed at 180°C or higher, and it was revealed by SEM observation that in these moldings wood flour particles adhere to each other. The specific bending strength was maximum for the moldings obtained from 180°C-steamed flour for both types of wood.     

东江干流河水的来源、水质及水资源保护

为了解东江河水的来源和水质状况,沿东江干流采集河水样品,分析河水中的主要离子浓度以及氢和氧的同位素比。结果表明,河水氢同位素比（6D）为一48．9‰～-39．8‰,氧同位素比（δ^18O）为-6．3‰～-5．7‰。根据氢氧同位素的相关关系,以河源市和汝湖镇为界,将研究范围内的东江干流分为Ⅰ、Ⅱ和Ⅲ段。Ⅰ段和Ⅱ段河水主要来自流域内的大气降水,由于河水与含有较高氧同位素比的岩石发生氧元素交换,使Ⅱ段河水呈现氧同位素漂移。Ⅲ段河水主要来自象头山和罗浮山山区的降雨并通过地下水补给。由于河水本身的稀释作用,东江河水中各离子浓度很低。人类活动相对频繁的东江下游的离子总量远大干上游和中游地区,说明人类活动对东江河水存在着潜在的影响。东江河水的污染来源可分为点源污染和面源污染,针对这两种污染分别提出了相应的措施。另外,香港饮用水与北部山区的地下水有密切关系,为保持香港水源的良好水质,应把保护北部山区的地下水列为战略重点。     

Intravaginal administration of estradiol benzoate capsule for estrus synchronization in goats

Estrus synchronization requires multiple treatments of hormonal drugs, requiring considerable time and cost. The aim of the present study was to develop an estrus synchronization protocol using intravaginal administration of estradiol benzoate (EB) capsules in goats. Two types of capsules were prepared: an EB capsule that melted immediately after administration and a sustained-release (SR) EB capsule that dissolved slowly and reached a peak after 24 h. Goats with functional corpus lutea were intramuscularly treated with prostaglandin F_2α (PG). At 24 h after PG administration, goats were administered 1 mg of EB solution intramuscularly (PG + 24IM; n = 6) or 1 mg of EB capsule intravaginally (PG + 24EB; n = 6). The SR EB capsule was administered intravaginally at the time of PG administration (PG + SR; n = 6). The control group (n = 6) received only PG. All groups showed estrus within 72 h after PG administration. The onset of estrus did not differ significantly between the PG + 24IM and PG + SR groups but was earlier than in the control group. Estradiol concentration in the PG + SR group peaked at 11.5 ± 6.1 h after EB and PG administration. Peak estradiol concentrations were not significantly different between the PG + 24IM and PG + SR groups (78.0 ± 25.8 and 64.0 ± 38.1 pg/ml, respectively), and were higher than the PG + 24EB and control groups (27.3 ± 8.8 and 14.6 ± 6.1 pg/ml, respectively). These results suggest that intravaginal administration of an EB capsule with a sustained-drug release base is applicable for estrus synchronization, as an alternative to intramuscular administration.     

Heart rate variability after horse trekking in leading and following horses

Horse trekking (HT) is having a stroll on a horse along a walking trail in a forest, field, and/or sandy beach. Generally in HT, horses exercise in tandem line outside the riding facilities. Because the leading horse will be confronted with stressors in the forefront, we hypothesized that the leading horse shows higher stress responses than the following one. In order to verify the hypothesis, we compared short‐term stress responses between each position in six horses. Exercise consisted of 15 min of ground riding and 45 min of HT with walking and trotting. Heart rate variability was analyzed for 5 min at 30, 60, and 90 min after the exercising period. There was no significant difference in heart rate during exercise between leading and following positions. The high frequency / low frequency power band of heart rate variability, an index of sympathetic nervous activity, after exercise, tended to be higher in the leading position than following one (P < 0.1). The result in this study can suggest that the leading horse was in a higher stressed state than the following horse after HT.     

Severe hypoplasia of the omasal laminae in a Japanese Black steer with chronic bloat--a case report

An 11-month-old Japanese Black steer with chronic bloat underwent clinical and histological analyses. During the observation period, it showed normal appetite and fecal volume but persistent chronic bloat symptoms. Compared to controls, the steer's feces contained undigested large straws. Necropsy revealed normal rumen, reticulum, and abomasum but a small omasum. The rumen, reticulum, and abomasum mucosa was normal, with well-developed ruminal papillae. However, severe hypoplasia of the omasal laminae was observed along with hypoplasia reticular groove and ruminoreticular fold. The contents of the reticulum, omasum, and abomasums comprised undigested large sized hay particles. The omasum papillae showed no pathological abnormalities. This is a rare case of a steer with chronic bloat probably caused by severe hypoplasia of the omasal laminae.     

A cat with myelodysplastic syndrome by administration of the methylation inhibitor Azacytidine

A 5-year-old female cat with nonregenerative anemia and thrombocytopenia was diagnosed with myelodysplastic syndromes (MDS), since peripheral blood and bone marrow (BM) examination revealed various dysplasias and a blast ratio of 19%. Chemotherapy with azacytidine (AZA; 70–35 mg/m², 3–5 days, three cycles) and treatment with prednisolone, antibiotics, and vitamin K2, and blood transfusion were performed. On day 106, blast cells and dysplasia had decreased in the BM, and the cat remained alive for at least 1,474 days. This report is the first on feline MDS treated with AZA, suggesting appropriate drug dosage, interval and effective combination should be investigated and the pharmacological and cell biological mechanisms needs to be elucidated in the future.     

Effect of chickpea husk dietary supplementation on blood and cecal parameters in rats

Chickpea husk was functionally evaluated for antioxidant status, blood parameters, cecal fermentation and microbial profiles in rats. Fifteen male rats (5 weeks of age) were divided into three groups; they were individually housed and fed one of the following diets for 3 weeks: purified diet containing 5% cellulose (Cellulose), an identical diet in which cellulose was replaced by corn starch (Starch) or by chickpea husk (Chick). Rats were sacrificed to obtain blood and cecal digesta samples. Chickpea husk contained high polyphenolic content and significant superoxide dismutase and 2,2‐diphenyl‐picrylhydrazyl scavenging activities. In a feeding experiment, Chick showed lowered cholesterol levels and improved antioxidant activity represented by reduced thiobarbituric acid reactive substances in blood. Chick showed increased cecal levels of total short chain fatty acids and butyrate, leading to a lower pH. Chick presented with lowered cecal indole and skatole concentrations, as did Cellulose. Cecal bacterial changes were notable in Chick, evidenced by differences in denaturing gradient gel electrophoresis banding patterns. However, representative bacteria quantified by real‐time PCR assay did not support this bacterial change. These results indicate that chickpea husk feeding can improve the antioxidative status of rats through its polyphenolic components and modulate the hindgut environment by its fibrous components.