Characterization of monoclonal antibodies against canine P-selectin glycoprotein ligand-1 (PSGL-1)

Thirteen different monoclonal antibodies against canine P-selectin glycoprotein ligand-1 (cPSGL-1) were obtained by immunization of rats with cells of a canine lymphoma cell line (Ema). O-sialoglycoprotein endopeptidase treatment of Ema cells showed that all of these antibodies recognized O-glycosylated peptides of canine PSGL-1. Experiments using deletion or point mutants of cPSGL-1 indicated that these antibodies could be categorized into several groups based on their cPSGL-1 recognition characteristics. These anti-cPSGL-1 monoclonal antibodies will be useful for analysis of the canine P-selectin and PSGL-1 system.     

Nutrient starvation affects expression of LC3 family at the feto-maternal interface during murine placentation

LC3 − the mammalian homolog of Atg8 − was found as autophagosome membrane binding protein in mammals and widely used as an autophagosomal marker. LC3A, B and C show different expression patterns in each tissue. The aim of this study was to reveal the differences of expression patterns among LC3 families in mouse placenta under normal condition and nutrient starving condition. LC3A and B were highly expressed in decidual cells. LC3A and B were increased in D14 compared with D12 and D16 in mouse placenta, while LC3C was decreased. Starvation induced increase in LC3B expression specifically. Immunohistochemistry showed different expression patterns among LC3A, B and C. LC3A expression in syncytiotrophoblast was vanished by starvation. The results of real time RT-PCR suggested differences between D12 and D16 in autophagic cascade induced by starvation. Taken together, this study suggests that autophagy could play a role in placental invasion system and that nutrient starvation affects LC3B expression.     

高寒退化草地恢复中种子包衣对出苗的影响

采用植物性吸水材料对垂穗披碱草(Elymus nutant)和山野豌豆(Vicia amoena)种子进行包衣处理,在模拟高寒草地条件下观察包衣种子的出苗,同时测定包衣混合物的持水力.结果表明,最适条件下两种牧草种子的出苗率分别为86%和88%;在模拟条件下,种子的出苗率分别为38%和24%.经过包衣处理后,种子的出苗...     

Hardness distribution on wood surface

For better understanding wood hardness, we developed a new hand-operated hardness tester that works with Brinell's method. With this tester we investigated the hardness distribution of wood minutely using a 2mm diameter ball tip. The following results were obtained: (1) On preliminary examination with medium-density fiberboard, we found that the value of Brinell's hardness decreased with the increase in the tip ball's diameter but that it was almost constant with the indenting velocity. (2) By using a small diameter tip, the difference of the hardness became clearer between earlywood and latewood. (3) With radial and tangential sections, we obtained hardness distribution patterns similar to those of wood grain. It seems that the hardness distribution reflects the distribution of density on wood surfaces.     

Hereditary changes in Capsicum annuum L. I. Induced by ordinary grafting

Summary Hereditary changes of certain Mendelian traits in red pepper after grafting are described. It is suggested that these changes are induced by a mechanism similar to that of transformation in higher organisms.The abstracts of this paper and the parts to follow have been presented at the XIII International Congress of Genetics held in August 1973 at Berkeley, California, USA as both a contributed paper and an exhibit including real herbarial specimen and color pictures of the variants genetically analyzed.     

Cadmium contamination of soils and rice plants caused by zinc mining V. Removal of soil cadmium by an HCl-leaching method for the control of high Cd rice

Abstract

A method of leaching polluted soil with diluted HCl solution was investigated for controlling high Cd in rice. Results obtained in the pot and field experiments are summarized as follows:

1. By leaching the soil in the pot with 0.05 or 0.1 N HCl solution followed by washing with water, soluble heavy metals were remarkably removed in the order of Cd>Cu>Zn?Pb; the Cd content decreased to half with 0.1 N HCI treatment.

2. Heavy metal contents of the plants decreased with the treatments. Especially, Cd content in straw and rice of the soil applied with CaCO₃ after the treatment was one-third and one-fifteenth of the control, respectively.

3. A contaminated paddy field was similarly treated using industrial cone. HCl. One-fourth of the soluble Cd in the surface soil was removed by this method, and the Cd content of the unpolished rice decreased from 0.33 ppm to 0.06 ppm in the plot treated with fused magnesium phosphate and calcium silicate after the treatment.

4. Cd concentration of the drained water from the treated field could soon be diluted far below the allowable limit with the abundant river current. A sufficient supply of calcareous materials was needed to compensate for the lost bases in the soil.