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排序方式: 共有454条查询结果,搜索用时 31 毫秒
411.
412.
Takayuki Matsuura Hirosuke Shinohara Yasuhiro Inoue Koji Azegami Seiya Tsushima Takanori Tsukamoto Akifumi Mizuno 《Journal of General Plant Pathology》2007,73(1):53-58
The phylogenetic relationships among Erwinia amylovora biovar 4 (the pathogen of bacterial shoot blight of pear in Japan), other biovars of E. amylovora, and Erwinia pyrifoliae were investigated using the sequences of 16S rRNA, gyrB, and rpoD genes. The tested isolates formed two distinct monophyletic groups in the phylogenetic trees constructed based on the gyrB gene, rpoD gene, or a combination of the three genes: group 1 contained E. amylovora biovars 1, 2, and 3; group 2 contained E. amylovora bv. 4 and E. pyrifoliae. This phylogenetic analysis showed that E. amylovora bv. 4 was more closely related to E. pyrifoliae than to other biovars of E. amylovora.
The nucleotide sequence data reported are available in the DDBJ/EMBL/GenBank databases under the accession numbers AB242876
to AB242925. 相似文献
413.
Mana Iwamoto Yoji Takeuchi Yasuhiro Takada Shintaro Kohno Isao Matsumoto Naoto Yamaoka 《Journal of General Plant Pathology》2007,73(1):38-40
To determine whether the cuticle of the barley coleoptile is responsible for a rise in appressorial turgor pressure in Blumeria graminis, we determined the appressorial turgor pressure by measuring cytorrhysis and plasmolysis in the presence of PEG6000. Appressorial
turgor pressure significantly increased 13–14 h after inoculation. On the other hand, when the cuticle was completely removed
from the barley coleoptile surface with diethyl ether, turgor pressure did not increase. Moreover, when we then recoated the
surface with the exogenous barley cuticle fraction, appressorial turgor pressure significantly increased 12–13 h after inoculation.
These results suggest that the cuticle on the surface of the barley coleoptile is necessary for the increase in the appressorial
turgor pressure. 相似文献
414.
Koji Azegami Takanori Tsukamoto Takayuki Matsuura Yasuhiro Inoue Hiroshi Uematsu Tatsuji Ohara Akifumi Mizuno Kouji Yoshida Hideo Bessho Shigeyoshi Sato Shigeru Kimura Masao Goto 《Journal of General Plant Pathology》2006,72(1):43-45
Invasion of apple fruit by Erwinia amylovora from fruit-bearing twigs through the abscission layer at fruit maturation was examined. Erwinia amylovora (ca. 105 cfu) tagged with bioluminescence genes from Vibrio fischeri was deposited in artificial wounds on fruit-bearing twigs of apple trees grown in a containment greenhouse on September 22,
27, or October 5, 2004. On October 22, 176 apples were harvested and cut horizontally in half. The upper halves were stamped
on plates of selective medium, and the lower halves were flooded with iodine solution to assess maturity. All fruit were symptomless
and fully mature. The pathogen was recovered from 19 (10.8%) apples. The result showed that if at least ca. 105 cfu of E. amylovora are present in fruit-bearing twigs at the time of fruit maturation, the bacteria can pass through the abscission layer into
the fruit, even though the mature fruit lack symptoms. 相似文献
415.
Hidetoshi Minamiyama Masafumi Shimizu Hitoshi Kunoh Tamotsu Furumai Yasuhiro Igarashi Hiroyasu Onaka Ryuji Yoshida 《Journal of General Plant Pathology》2003,69(1):65-70
An endophytic actinomycete, isolate R-5 of Streptomyces galbus Frommer, that has promising potential as a biocontrol agent was originally isolated from field-grown rhododendron. In this
study, the mode of entry of R-5 into leaves of tissue-cultured seedlings of rhododendron was investigated in connection with
its production of cell wall-degrading enzymes. Light and scanning electron microscopy (SEM) revealed that R-5 grew on leaf
surfaces and entered leaf tissues via stomata and that the internal mycelia grew out of stomata after colonization in host
tissues. Micromanipulation at the SEM level demonstrated a prominent depression in the host surface at the interfaces with
the mycelia, suggesting that such a depression could be caused by degradation of cell wall components by hydrolytic enzymes
secreted from R-5 mycelia. In subsequent plate assays, R-5 produced cellulase, pectinase, xylanase, and nonspecific esterase
when cultured in liquid medium. Moreover, R-5 multiplied in mineral medium containing cellulose, pectin, or xylan as a single
carbon source. Thus, R-5 mycelia could degrade host cell walls at contact sites and probably utilize the degradation products
as carbon sources.
Received: May 16, 2002 / Accepted: July 9, 2002 相似文献
416.
417.
Takuya Maruo Yasuhiro Fukuyama Yuta Nishiyama Yuki Nemoto Eiichi Kanai Shinpei Kawarai Hideki Kayanuma Kensuke Orito 《The Canadian veterinary journal. La revue veterinaire canadienne》2021,62(10):1117
Intraoperative acridine orange-photodynamic therapy (AO-PDT) and cribriform plate irradiation are used to treat canine intranasal tumors. The purpose of this study was to evaluate the effects of AO-PDT on intranasal tumors and the recurrence rate of tumors after this treatment. Treatments with AO-PDT were performed on 38 dogs through a narrow window of the dorsal nasal cavity. The median progression-free interval was 12 mo and recurrence was detected in 21 dogs. Based on computed tomography, recurrence in 16 dogs was biased to the following areas: lateral (n = 10), medial (n = 2), ventral (n = 0), rostral (n = 0), and caudal (n = 8). Side effects were mild and included subcutaneous emphysema and rhinitis. The median survival time was 24 mo. Although AO-PDT with cribriform irradiation is an effective treatment for intranasal tumors, AO-PDT techniques should be improved to treat the nasal cavity more uniformly and thoroughly. 相似文献
418.
Haruyama J Ohtake M Matsunaga T Morota T Honda C Yokota Y Abe M Ogawa Y Miyamoto H Iwasaki A Pieters CM Asada N Demura H Hirata N Terazono J Sasaki S Saiki K Yamaji A Torii M Josset JL 《Science (New York, N.Y.)》2009,323(5916):905-908
We determined model ages of mare deposits on the farside of the Moon on the basis of the crater frequency distributions in 10-meter-resolution images obtained by the Terrain Camera on SELENE (Selenological and Engineering Explorer) (Kaguya). Most mare volcanism that formed mare deposits on the lunar farside ceased at approximately 3.0 billion years ago, suggesting that mare volcanism on the Moon was markedly reduced globally during this period. However, several mare deposits at various locations on the lunar farside also show a much younger age, clustering at approximately 2.5 billion years ago. These young ages indicate that mare volcanism on the lunar farside lasted longer than was previously considered and may have occurred episodically. 相似文献
419.
Shirin Farzadfar Yasuhiro Tomitaka Mutsumi Ikematsu Ali Reza Golnaraghi Reza Pourrahim Kazusato Ohshima 《European journal of plant pathology / European Foundation for Plant Pathology》2009,124(1):45-55
Eight provinces of Iran were surveyed during 2003–2008 to find Brassicaceae reservoir weed hosts of Turnip mosaic virus (TuMV). A total of 532 weed samples were collected from plants with virus-like symptoms. The samples were tested for the
presence of TuMV by enzyme-linked immunosorbent assay using specific antibodies. Among those tested, 340 samples (64%) were
found to be infected with TuMV. Rapistrum rugosum, Sisymberium loeselii, S. irio and Hirschfeldia incana were identified as the Brassicaceae weed hosts of TuMV, and the former two plant species were found to be the most important
weed hosts for the virus in Iran. The full-length sequences of the genomic RNAs of IRN TRa6 and IRN SS5 isolates from R. rugosum and S. loeselii were determined. No evidence of recombination was found in both isolates using different recombination-detecting programmes.
Phylogenetic analyses of the weed isolates with representative isolates from the world showed that the IRN TRa6 and IRN SS5
isolates fell into an ancestral basal-Brassica group. This study shows for the first time the wide distribution and phylogenetic relationships of TuMV from weeds in the
mid-Eurasia of Iran. 相似文献
420.
Yasuhiro Ishiga Srinivasa Rao Uppalapati Takako Ishiga Carol L. Bender 《Journal of General Plant Pathology》2010,76(3):188-195
Coronatine (COR) functions as a virulence factor in the interaction of Pseudomonas syringae pv. tomato strain DC3000 with tomato and Arabidopsis. COR consists of two moieties, coronafacic acid (CFA) and coronamic acid (CMA).
Both COR and CFA function as structural and functional analogues of jasmonic acid (JA) and related signaling compounds such
as methyl jasmonate (MeJA) and JA-isoleucine (JA-Ile). The precise function of COR and whether MeJA functions as an analogue
of COR in disease development are not known. In this study, we analyzed whether the COR-defective mutant DB29, which is a
CFA− CMA− mutant of DC3000, could be complemented for virulence via the exogenous application of COR, CFA, or MeJA. When tomato seedlings
were inoculated with DB29 and supplemented with exogenous COR, the DB29 population multiplied in tomato seedlings and induced
disease phenotypes similar to wild-type DC3000. Although the addition of exogenous MeJA or CFA enhanced the multiplication
of DB29, wild-type disease phenotypes could not be restored with these compounds. Furthermore, inoculation of DB29 along with
exogenous COR, but not MeJA or CFA, suppressed the expression of defense-related genes and increased the accumulation of reactive
oxygen species, which are associated with severe chlorosis. Taken together, our results suggest that although COR targets
the jasmonate pathway by mimicking JA, the function of COR in disease development is distinctly different from MeJA or CFA. 相似文献