Effect of Weed Removal on Productivity of Chickpea (Cicer arietinum L.) and Lentil (Lens culinaris Med.) in a Mediterranean Environment

Three field experiments were conducted on chickpea ( Cicer arietinum L.) and four on lentil ( Lens culinaris Med.) at different winter-sown rainfed locations in Jordan from 1988/89 to 1990/91 to study the effect of the duration of weed-free and weed-infested conditions on yields and yield components of the crops. Chickpea seed yields were reduced on average by 81 % and straw yields by 63 % when fields remained weed infested until harvest compared with weed-free conditions throughout the growing season. The corresponding lentil seed and straw yield decreases were 63 % and 55 %. As the duration of weed-free period increased and the duration of weed-infested period decreased, yields increased. However, the critical period of weed interference was between 35 and 49 days after emergence in chickpea and between 49 and 56 days after emergence in lentil, when these crops were at an advanced stage of vegetative growth. There were significant negative correlations between the weed dry weight and the seed or straw yields. The reduction in seed yields in both crops because of weed interference occurred mainly through the reduced number of pods /plant, which in turn was partly the result of reduced number of secondary branches. In chickpea, some reduction also occurred through reduced 100-seed weight.     

Efficacy of an inactivated recombinant vaccine encoding a fimbrial protein of Pasteurella multocida B:2 against hemorrhagic septicemia in goats

This study was carried out to determine the antibody responses and protective capacity of an inactivated recombinant vaccine expressing the fimbrial protein of Pasteurella multocida B:2 following intranasal vaccination against hemorrhagic septicemia in goats. Goats were vaccinated intranasal with 10⁶ CFU/mL of the recombinant vaccine (vaccinated group) and 10⁶ CFU/mL of pET32/LIC vector without fimbrial protein (control group). All three groups were kept separated before all goats in the three groups were challenged with 10⁹ CFU/mL of live pathogenic P. multocida B:2. During the course of study, both serum and lung lavage fluid were collected to evaluate the antibody levels via enzyme-linked immunosorbent assay. It was found that goats immunized with the inactivated recombinant vaccine developed a strong and significantly (p < 0.05) higher specific IgA and IgG responses in both serum and lung lavage fluid samples compared to the control and unvaccinated groups. Following intratracheal challenge, the rate of isolation was 17% for the vaccinated group, 67% for the control group and 100% for the unvaccinated group. However, none of the goat from the vaccinated group had P. multocida B:2 in the liver, tonsil and heart. Therefore, the study revealed that an inactivated recombinant vaccine significantly provides significant protection against high dose challenge and enhances the stimulation of the local and systemic immunities.