全文获取类型
收费全文 | 18563篇 |
免费 | 936篇 |
国内免费 | 1967篇 |
专业分类
林业 | 1678篇 |
农学 | 2078篇 |
基础科学 | 1289篇 |
2772篇 | |
综合类 | 6706篇 |
农作物 | 1112篇 |
水产渔业 | 784篇 |
畜牧兽医 | 2834篇 |
园艺 | 949篇 |
植物保护 | 1264篇 |
出版年
2024年 | 88篇 |
2023年 | 225篇 |
2022年 | 616篇 |
2021年 | 763篇 |
2020年 | 726篇 |
2019年 | 722篇 |
2018年 | 492篇 |
2017年 | 753篇 |
2016年 | 620篇 |
2015年 | 919篇 |
2014年 | 881篇 |
2013年 | 1144篇 |
2012年 | 1368篇 |
2011年 | 1523篇 |
2010年 | 1390篇 |
2009年 | 1176篇 |
2008年 | 1243篇 |
2007年 | 1177篇 |
2006年 | 1005篇 |
2005年 | 814篇 |
2004年 | 484篇 |
2003年 | 370篇 |
2002年 | 391篇 |
2001年 | 402篇 |
2000年 | 386篇 |
1999年 | 278篇 |
1998年 | 239篇 |
1997年 | 195篇 |
1996年 | 196篇 |
1995年 | 206篇 |
1994年 | 145篇 |
1993年 | 129篇 |
1992年 | 99篇 |
1991年 | 78篇 |
1990年 | 57篇 |
1989年 | 59篇 |
1988年 | 33篇 |
1987年 | 21篇 |
1986年 | 11篇 |
1985年 | 9篇 |
1984年 | 6篇 |
1983年 | 8篇 |
1982年 | 7篇 |
1981年 | 3篇 |
1980年 | 1篇 |
1963年 | 1篇 |
1962年 | 1篇 |
1956年 | 2篇 |
1955年 | 4篇 |
排序方式: 共有10000条查询结果,搜索用时 31 毫秒
991.
Qi-Ya Zhang Zhe Zhao Feng Xiao Zheng-Qiu Li Jian-Fang Gui 《Aquaculture (Amsterdam, Netherlands)》2006,251(1):1-10
Three Rana grylio virus (RGV) isolates and lymphocystis disease virus (LCDV-C) were molecularly characterized by antigenicity comparison, Western blot detection of viral polypeptides, restriction fragment length polymorphism analysis of viral genomes, and MCP sequence analysis. Significant antigenicity differences existed among the three RGV isolates and LCDV-C. Western blot detection indicated that the viral polypeptides of three RGV isolates could be recognized by the anti-RGV9807 serum, whereas no bands were observed in the LCDV-C, and significant differences exist among the band patterns of three RGV isolates. Restriction fragment length polymorphism (RFLP) analysis was performed by digesting genomic DNA of the four iridovirus isolates with restriction endonucleases HindIII, KpnI, XbaI and BamHI. On the whole, obvious discrepancies existed between LCDV-C and RGV isolates, and some significant band pattern differences were also revealed between RGV9808 and RGV9506 (or RGV9807) in the profiles of restriction endonucleases XbaI, KpnI and BamHI. PCR amplification and sequence analysis of MCP gene sequence further revealed their phylogenetic relationship among the three RGV isolates, LCDV-C and other iridoviruses. RGV9506, RGV9807 and RGV9808 are clustered together with other ranaviruses, such as FV3, BIV, TFV and ENHV, although the RGV9808 is more close to EHNV than to other ranaviruses. Additionally, LCDV-C is clustered with LCDV-1, the type species of genus Lymphocystisvirus. The current study provides clear evidence that significant genetic difference exists among the three RGV isolates. Therefore, further work on comparative genomic studies will contribute significantly to understanding of their taxonomic position and pathological mechanism. 相似文献
992.
993.
泌乳后期奶牛干物质及部分营养需要研究 总被引:1,自引:0,他引:1
选择15头中国荷斯坦奶牛,随机分成三组,研究河北农区泌乳后期奶牛干物质及部分营养需求模型。结果表明:泌乳后期奶牛干物质采食量DM(kg)=0.0685W0.75+0.45(0.4×M+15×fat);所需产奶净能NE(LkJ)=376.45W0.75+3138×(0.4×M+15×fat)+ΔW×25100;可消化蛋白质DCP(g)=3.44W0.75+M×CP%/0.6+ΔW×325;各项指标略高于中国奶牛饲养标准(NY/T34-2004)。 相似文献
994.
995.
"对口支援西部地区高等学校计划"是促进教育区域协调发展的重要措施,是具有中国特色的促进欠发达地区教育发展的模式之一。通过对口支援,新疆农业大学在教学、科研、师资队伍建设等方面均取得了长足发展。新疆农业大学和支援高校间的援助实践表明,推动对口支援工作深入开展,需要提高思想认识,健全考核评价机制,形成支、受援高校双赢的长效机制,设立对口支援专项资金,发挥受援学校所在地行政部门的作用。 相似文献
996.
AIM: To investigate the relationship between the expression of estrogen receptor α36 (ER-α36) and the invasion of gastric cancer cells. METHODS: SGC7901 cells were treated with 17β-estradiol at high or low concentration. The invasion of gastric cancer cells and the expression of ER-α36 were detected. The SGC7901 cells with the characteristics of stable low expression and high expression of ER-α36 were constructed. The invasion ability and microRNA sequences were determined in above-mentioned recombinant cells. RESULTS: The decreased invasion ability and ER-α36 expression were detected in SGC7901 cells treated with low concentration of 17β-estradiol. The situation was the opposite in the cells treated with high concentration of 17β-estradiol. The expression of miR-143 was significantly decreased in the SGC7901 cells with stable high expression of ER-α36 and was increased in the SGC7901 cells with stable low expression of ER-α36. CONCLUSION: The expression of ER-α36 is positively related to the invasion of gastric cancer cells. It is possible that miR-143 plays an important role in the regulation of gastric cancer invasion. 相似文献
997.
AIM: To investigate how the function of retinal ganglion cells (RGCs) change in the midterm of retinal pigmentosa (RP) in rd1 mice (a transgenic animal model of RP). METHODS: The action potentials from multiple RGCs in rd1 mice at postnatal 20 d (P20) or normal C57 mice (control) were simultaneously recorded by multi-electrode array recording. The functional changes of surviving ganglion cells were evaluated by comparing spontaneous and light-evoked activities of RGCs between rd1 and control mice. The extent of photoreceptor degeneration was verified by immunohistochemical staining. RESULTS: Immunohistochemistry results showed the thickness of the retinal photoreceptor layer of rd1 mice was significantly lower than that in normal mice at P20. According to the light response properties, we classified ganglion cells into 6 subgroups: ON sustained, ON transient, ON-OFF sustained, ON-OFF transient, OFF sustained and OFF transient RGCs, with a very tiny percentage of OFF sustained RGCs (1.0%~3.1%). The percentage of RGCs remaining light responsive in rd1 mice was significantly lower than that in C57 mice. The average spontaneous spiking rate for rd1 RGCs was overall significantly increased compared to that in C57 cells, whereas different RGC types had different changes. The light-induced responses and light sensitivities of all types of RGCs in rd1 mice were both significantly lower than those in C57 mice. CONCLUSION: The photoreceptors of rd1 mice are severely degenerated in the midterm of retinal degeneration. The functions of RGCs in rd1 mice in the midterm of degeneration decay obviously, with variance in different RGCs types. 相似文献
998.
随着技术的进步发展,这门学科的内容没有显著的变化和发展,其关键因为在于它是机械加工行业基础.通过加强学生对钳工技能的认识、培养学生积极学习的主观能动性、加强学生分析问题和解决问题等方面的能力培养,使学生在有限的时间内掌握更多的操作技能. 相似文献
999.
兰州大浪沟雨养生态试验示范区土壤养分状况研究 总被引:1,自引:1,他引:0
【目的】为了对兰州雨养生态区植被恢复与重建提供理论依据.【方法】以兰州市城关区青白石乡大浪沟为试验示范区,研究不同坡向(阴坡、半阳坡和阳坡)、不同土层深度(0~20、20~40、40~60、60~80cm)土壤养分的变化规律.【结果】0~80cm土层,土壤养分含量表聚现象明显,表现为,随着土层深度的增加而降低,且阴坡半阳坡阳坡.阴坡、半阳坡和阳坡的土壤有机质平均含量分别为:5.69、4.36、3.09g/kg;有效磷(P)平均含量分别为14.13、14.08、13.85mg/kg;速效钾(K)平均含量分别为:159.13、158.76、150.14mg/kg;水解氮(N)平均含量分别为:26.4、19.7、14.7mg/kg.【结论】大浪沟雨养生态试验示范区土壤养分含量总体偏低,土壤中速效K含量较高,有效P含量中等,有机质和水解N的含量极低,建议在植被恢复配置时,考虑耐瘠薄的品种,并适当施用有机肥料. 相似文献
1000.