Ballistic 2-d imaging through scattering walls using an ultrafast optical kerr gate

An ultrafast optical shutter was used to image ultrasmall objects hidden behind scattering walls by a procedure that selects in time the ballistic component and rejects the scattered diffusive light. Scattering walls used in this experiment included human breast tissue, chicken breast tissue, and a water suspension of polystyrene particles with scattering coefficients up to 21.7. Submillimeter resolution was achieved for two-dimensional ballistic images of a single point, a double-point fluorescence source, and a bar test chart in or behind these different turbid media.     

2005-2020年转Bt基因棉花抗虫性变化及其与产量性状的相关性分析

为了给转Bt基因棉花在科研和生产上多世代可持续利用提供依据,以转Bt基因棉花品种银山8号为试验材料,从2005年开始,连续16年进行跟踪研究。结果表明,随种植世代的增加,生物测定银山8号第2～4代棉铃虫幼虫校正死亡率、铃期叶片和铃期小铃杀虫蛋白表达量均有不同程度的线性上升趋势,同时皮棉产量线性回归也呈增长趋势;苗期叶片、蕾期叶片和蕾期小蕾杀虫蛋白表达量呈线性下降趋势。相关性分析表明,抗虫棉的抗虫性与皮棉产量间均呈正相关,株铃数和衣分是构成皮棉产量的重要因子,与皮棉产量的相关性分别达到极显著和显著水平。外源Bt基因转入棉花后能稳定遗传给后代,通过卡那霉素鉴定和系统选择可以持续保持转Bt基因棉花多世代的抗虫性,甚至通过提纯复壮逐年优选,可实现转Bt基因抗虫棉特定生育时期或部分棉花器官抗虫性的提高。     

钛合金表面火焰喷焊镍基耐磨涂层的组织和性能

采用火焰喷焊技术在T i 6A l 4V合金基体上制备了镍基耐磨涂层,研究了其显微组织、合金元素的扩散、显微硬度和干摩擦磨损性能。结果表明,喷焊涂层具有韧基体加硬质相的耐磨组织;涂层横切面的组织、显微硬度和合金元素的扩散沿层深方向呈现连续性和渐变性,基体和涂层表现为冶金结合;喷焊表层的显微硬度为933.2 HV,是基体钛合金的3倍以上;涂层在不同条件下的磨损量都较小,耐磨性有较大的提高,转速和温度对其耐磨性能影响较大。     

三峡库区主要植被生物量与生产力分配特征

在调查104块样地的基础上,利用95株标准木的树干解析数据,选择主要生物量生长模型,对三峡库区主要植被生物量和生产力的分配规律进行研究.结果表明:1)在各树种生物量组成中,干材生物量最大,占总生物量的44.46%～63.07%,而皮、枝和根生物量比例间的大小关系因树种不同而有所差异;2)所有树种干生物量比例与枝和叶生物量比例存在显著的线性负相关,而枝和叶生物量之间存在明显的正相关,随着单株林木生物量增加,针叶树种干和皮生物量比例会随之增加,但枝、叶和根的生物量比例则有所下降;3)各林分生物量为75.38～191.82t·hm-2,乔木层(67.57～179.00 t·hm-2)>枯落物层(3.95～11.61 t·hm-2)>灌木层(2.40～8.35 t·hm-2),乔木层和枯落物层生物量低,有利于灌木层生物量的生长;4)各林分生产力为4.78～11.07 t·hm-2a-1,常绿阔叶林最高,柏木林最低,混交林的生产力高于纯林,其中针阔混交林的生产力又高于针叶混交林.     

Relationship between stable carbon isotope discrimination and water use efficiency under regulated deficit irrigation of pear-jujube tree

To investigate the relationship between stable carbon isotope discrimination (Δ) of different organs and water use efficiency (WUE) under different water deficit levels, severe, moderate and low water deficit levels were treated at bud burst to leafing, flowering to fruit set, fruit growth and fruit maturation stages of field grown pear-jujube tree, and leaf stable carbon isotope discrimination (Δ_L) at different growth stages and fruit stable carbon isotope discrimination (Δ_F) at fruit maturation stage were measured. The results indicated that water deficit had significant effect on Δ_L at different growth stages and Δ_F at fruit maturation stage. As compared with full irrigation, the average Δ_L at different growth stages and Δ_F at fruit maturation stage were decreased by 1.23% and 2.67% for different water deficit levels, respectively. Δ_L and Δ_F among different water deficit treatments had significant difference at the same growth stage (P < 0.05). Under different water deficit conditions, significant relationships between the Δ_L and WUE_i (photosynthesis rate/transpiration rate, P_n/T_r), WUE_n (photosynthesis rate/stomatal conductance of CO_2, P_n/g_s), WUE_y (yield/crop water consumption, Y/ET_c) and yield, or between the Δ_F and WUE_y and yield were found, respectively. There were significantly negative correlations of Δ_L with WUE_i, WUE_n, WUE_y and yield (P < 0.01) at the fruit maturation stage, or Δ_L with WUE_i and WUE_n (P < 0.01) over whole growth stage, respectively. Δ_F was negatively correlated with WUE_y, WUE_n and yield (P < 0.05), but positively correlated with ET_c (P < 0.01) over the whole growth stage. Thus Δ_L or Δ_F can compare WUE_n and WUE_y, so the stable carbon isotope discrimination method can be applied to evaluate the water use efficiency of pear-jujube tree under the regulated deficit irrigation.     

大花蕙兰幼苗叶片诱导类原球茎

以大花蕙兰无菌幼苗叶片为外植体,成功诱导类原球茎(PLB).叶片部位是影响诱导的关键,只有叶片基部片段能成功诱导出PLB.不同激素配比以及外植体来源对PLB诱导有重要的影响.低浓度的2,4-D和较高浓度的6-BA对于拟球茎诱导PLB具有显著的促进作用.当2,4-D浓度高于0.6 mg/L时,PLB诱导受到显著抑制.较高的2,4-D水平可能有利于PLB诱导初始阶段,但不利于PLB诱导中后期的进一步发育.不同基因型有不同的最佳激素组合.添加0.2 mg/L的NAA对大花蕙兰PLB诱导没有显著影响,但在有的基因型中对PLB诱导有促进作用.以叶片基部片段诱导大花蕙兰PLB是可行的,为转基因研究提供了再生技术基础.     

综合加固法处理沿海滩涂软基施工

采用水泥粉喷柱、砂砾垫层与覆膜土工布三者结合的综合加固法处理滨海相淤泥沉积,水泥粉喷桩的施工及成桩检测;土工布的施工;软基处理应注意的问题。     

Molecular cloning and functional characterization of porcine IFN-beta promoter stimulator 1 (IPS-1)

The IFN-beta promoter stimulator 1 (IPS-1), also known as MAVS/VISA/Cardif, is an adaptor molecule for the retinoic-acid-inducible protein I (RIG-I) or melanoma-differentiation-associated gene 5 (MDA5) that recognizes intracellular double-stranded RNA (dsRNA) and triggers a signal for producing type I IFN. In the present study, porcine IPS-1 cDNA was cloned, using RT-PCR coupled with rapid amplification of cDNA ends (RACE)-PCR, from porcine peripheral blood mononuclear cells. The open reading frame of porcine IPS-1 consists of 1575bp encoding 524 amino acids. The putative porcine IPS-1 protein contains a N-terminal CARD-like domain, a central proline-rich domain, a C-terminal transmembrane domain, and exhibits similarity to mouse, rat, monkey, human and cattle counterparts, ranging from 59% to 79%. Semi-quantitative RT-PCR showed that porcine IPS-1 mRNA was widely expressed in different tissues. Porcine kidney (PK-15) cells transfected with a DNA construct encoding porcine IPS-1 produced type I IFN, and activated IRF3 and NF-kappaB. Deletion mutant analyses further revealed that both the CARD-like domain and transmembrane domain are essential for these functions. In addition, poly(I:C)-induced porcine IFN-beta promoter activation in PK-15 cells was significantly reduced by siRNA targeting IPS-1, indicating that IPS-1 is an important immunoregulator in the porcine innate immune system. The availability of porcine IPS-1 and establishment of its function in the type I IFN signaling pathway provides a useful molecule for defining its role during the course of pig infectious diseases.     

施氮量对不同水稻品种氮肥利用率与农艺性状的影响

为探明施氮量对不同水稻品种产量、氮肥利用率和农艺性状的影响,以适合江苏省种植的12个中粳稻（含籼/粳杂交稻）品种为材料,大田种植,设置0N（全生育期不施氮）、200N（全生育期施纯氮200kg/hm ²）和360N（全生育期施纯氮360kg/hm²）3个施氮量处理,研究各品种产量、氮肥利用率和一些农艺性状的差异。结果表明：甬优2640在3个施氮水平产量均最高,库容量大是其高产的主要原因。其他品种产量对氮肥响应的表现不一。在较低氮（0N、200N）条件下,淮稻13号和武运粳30的产量较高,宁粳1号和扬粳4038的产量较低。在高氮（360N）条件下,宁粳1号和扬粳4038产量较高。在较低施氮量条件下,产量和氮肥利用率较高的品种具有较高的干物质积累量、茎蘖成穗率、叶面积指数、粒叶比及群体生长速率,这些指标可作为筛选氮高效品种的农艺性状指标。     

SR100垂直摇臂式喷头摇臂有限元分析

针对垂直摇臂式喷头导流器几何结构及运动特性,研究了摇臂在运动过程中的受力及约束情况,并结合动力学理论计算公式,计算和分析了喷头在运动过程中载荷大小和约束情况.采用ANSYS Workbench软件,建立摇臂三维模型,对摇臂进行有限元模态分析、静力学分析及疲劳可靠性分析.通过有限元模态分析,得出各阶模态下摇臂的固有频率和振型,该摇臂的各阶振型主要表现为弯曲、扭曲及局部变形;当摇臂的工作频率小于41.785 Hz时,其振动以一阶振型为主.通过静力分析得出摇臂的应力分布情况,发现在摇臂安装导流器处存在较大的应力分布;通过对摇臂的疲劳分析,发现摇臂各部位安全系数差别很大,其最小值位于摇臂前端,为1.229 1,长期工作此处易发生裂纹或断裂.为提高摇臂的工作稳定性与可靠性,可以通过加强薄弱部位设计,或改进局部结构、提高材料的力学性能来实现.