2株可培养细菌氮代谢特性的研究

研究旨在分析土壤中可培养细菌菌株的氮代谢特征,并进一步探讨微生物在土壤氮素转化中的可能作用机制。以2株分离自苹果园土壤的细菌菌株SY5-4和SY11-10为试材,采用传统培养方法结合分子检测技术,分别测定菌株生长特性及其氮素转化能力。研究结果表明,异养条件下,菌株SY5-4和SY11-10的世代时间分别为243.5 min和202.7 min。菌株生长过程中,培养液中铵态氮浓度始终维持在较高水平,铵态氮、亚硝态氮和硝态氮浓度均表现出先升后降的趋势。硝化（amoA和hao）和反硝化（nosZ、norB、nirK和nap）基因检测结果表明,菌株SY11-10具有多种氮素转化潜能。综上,供试菌株培养过程中,培养液中氮素发生变化,并在菌体中检测到不同氮转化基因,表明菌株参与多种氮代谢途径。     

酸改性麦麸挂面配方的研究

为改善挂面制品的营养保健功能,研发谷物加工副产物的深加工产品,将经过超声波-酸解法改性处理后的麦麸粉添加到面粉中用于制作酸改性麦麸挂面。通过单因素试验及正交试验优化确定酸改性麦麸挂面的最优配方。结果表明,在面粉100 g的基础上,酸改性麦麸粉添加量为6%（相当于面粉质量的百分比）、水添加量为56%、食盐添加量为3.5%、食碱添加量为0.4%、羧甲基纤维素钠添加量为0.4%,此条件下制得的挂面品质良好,且外观、适口性较好。     

外来物种琵琶鼠鱼与本地物种鲫鱼混养的初步研究

试验探究了外来物种琵琶鼠鱼(Pterygoplichthys multiradiatus)和本地物种鲫鱼(Crucian carp)混养时的关系,研究入侵物种对生物多样性和生态环境的破坏。结果表明,琵琶鼠鱼与鲫鱼在不同配比的混养情况下,体重均表现为降低,其中琵琶鼠鱼的体重下降更多,但均有正增长的趋势。琵琶鼠鱼与鲫鱼之间存在空间竞争关系,且鲫鱼表现出更强的竞争优势。     

Cloning and Sequence Analysis of PTTG1 Gene in Luchuan Pig

This experiment was aimed to clone PTTG1 gene CDS sequence of Luchuan pig,and was analyzed by bioinformatics methods.A pair of special primers was designed according to predicted sequence of porcine PTTG1 in GenBank.The coding sequence of PTTG1 in Luchuan pig was amplified by RT-PCR,its gene sequence characteristics and protein structure was systemically analyzed by bioinformatics techniques.The results showed that the cloned PTTG1 fragment included a 609 bp CDS (coding 202 amino acids).The sequence multi-aligned results showed that Luchuan pig shared 90.15%,87.85%,87.52%,87.03%,76.03%,74.38%,55.74% and 44.48% of similar nucleotide sequence with that of Bos,Pan troglodytes,Homos,Macaca,Rattus,Mus,Gallus and Danio retio,respectively.The protein structure analysis results showed that the protein attributed hydrophilic protein without signal peptide,localized in cell cytoplasm and had 16 phosphorylation sites.The phylogentic tree of amino acid indicated that PTTG1 was highly conserved in the process of evolution of different species.The cloning and analysis of PTTG1 gene provided an important foundation for further study biological function of porcine PTTG1 during early embryonic development.     

细香葱周年高效生产技术

广西柳江县种植细香葱历史悠久，但在2004年以前，种植规模小，种植零散，经济效益不高。从2005年开始，随着种植结构的不断调整和优化，在当地政府的引导和当地经纪人、种植大户的带动下，细香葱栽培面积不断扩大，并且出现了三都镇里贡村、三都镇觉山村、三都镇龙兴村、进德镇沙子村等细香葱种植专业村和一大批种植专业户，     

基于加权Voronoi图的林木竞争指数

以福寿山林场25年生的杉木生态公益林为研究对象,通过分析林木竞争指数与胸径生长因子的相关关系,得出二者为负相关,从而确定加权Voronoi图的权重为胸径倒数(W),借助Arcgis软件里的加权Voronoi图工具,通过加权Voronoi图的方法来确定竞争单元,提出基于加权Voronoi图的W_V_Hegyi竞争指数,最后将W_V_Hegyi竞争指数与Hegyi、V_Hegyi竞争指数进行对比分析,结果表明:1) 3种竞争指数与胸径相关性强弱排序为:W_V_HegyiV_HegyiHegyi;2) 3种竞争指数与胸径服从幂函数关系,曲线拟合度排序为:W_V_HegyiV_HegyiHegyi;3) 样地竞争指数平均值排序均为:V_HegyiW_V_HegyiHegyi。通过以上比较分析证明,W_V_Hegyi竞争指数比Hegyi、V_Hegyi竞争指数能更准确地反映林木间的竞争关系。      

中国煤炭生产甲烷排放现状及对策研究

指出了煤炭行业的甲烷排放也是造成温室效应的重要因素之一,气候的变化直接影响到人类社会的可持续发展,我国有着丰富的煤层气储藏量,为发展新清洁能源奠定了坚实的基础,但是由于政策和技术限制,我国煤炭行业每年向大气中排放了大量的煤炭甲烷气体,尤其是低浓度煤炭甲烷,造成了资源浪费的同时也是煤炭生产安全的隐患.结合我国煤炭甲烷排放特征,分析了我国煤炭甲烷排放现状和主要的利用技术,并提出了中国煤炭甲烷排控的对策.     

反向遗传技术在牛轮状病毒研究中的应用

本文综述了牛轮状病毒的生物学特性、反向遗传学系统的建立及其研究进展,并对反向遗传技术应用于牛轮状病毒进行展望。     

Preparation of Adjuvant Diluent for Live Vaccine against Classical Swine Fever and Evaluation of its Immune Effects

The aim of this study was to develop a novel oil-in-water (O/W) emulsion as adjuvant diluents (AD) for live vaccine against classical swine fever (CSF) that could effectively enhance the immune effect of vaccine.The AD was prepared by high-pressure homogenization technique.Formulations and preparation parameters were optimized with response surface design.Its stability, particle size, polydispersity (PDI) and Zeta potential were characterized.The humoral immune response and cellular immune response of the AD were evaluated with BALB/c mice by intramuscular injection.The particle size of the AD prepared by optimized formulation and parameters was 100.4 nm, PDI was 0.147, and Zeta potential was —28.7 mV.The experiment results showed that the AD had good stability.The AD was inoculated combined with live vaccine against CSF into BALB/c mice by intramuscular injection.The results showed that the live vaccine against CSF specific immune responses could be evoked in mice by co-inoculation with AD and vaccine.The cellular immune response levels in co-inoculated groups were significantly higher than control group (P<0.05), with obvious phenomena of higher levels of IFN-γ, IL-6 and IL-4 in serum.The result revealed that cellular immune capability significantly improved with the AD.The results strongly revealed that cellular immune capability significantly improved by introducing AD for effective immune-adjuvant for live vaccine against CSF.