松嫩平原优势种羊草与其主要伴生种芦苇空间分布格局分析

芦苇是松嫩草地优势种羊草的主要伴生种,两者常常形成物种组成比较单一的混生群落或者与其他物种组成羊草 杂类草群落.选择羊草 芦苇混生群落,羊草群落和芦苇群落的交错区进行羊草、芦苇单物种格局分析.结果表明,1)混生群落和交错区中,羊草和芦苇均是以集聚分布的形式存在;2)羊草在混生群落中的格局强度小于交错区中的格局强度,芦苇在2种类型群落中的格局强度没有显著差异;3)除了考虑物种的生物学特性以外,混生群落种内、种间竞争也是影响羊草集聚分布和格局强度的主要因素,交错区中,环境异质性和种间竞争是影响集聚分布和格局强度的重要因子;4)芦苇在混生群落和交错区中格局强度没有明显差异,由此可以判定芦苇的空间分布格局形成主要受植物本身生物学特性的影响.     

山羊痘灭活疫苗免疫保护试验

将山羊痘GT4-STV42-56种毒在犊牛睾丸细胞上繁殖,收获毒液,用0.1%甲醛溶液灭活,加适量206佐剂,制成山羊痘油佐剂灭活疫苗.将疫苗以不同剂量皮下免疫接种不同种群的山羊.免疫后21 d,用AV40株强毒进行攻毒保护试验.结果显示,免疫剂量达0.5 mL时,疫苗对内蒙绒山羊的保护率为100%,对广西黑山羊的保护率为80%.实验表明,山羊痘灭活疫苗对山羊的免疫效果确实可靠.     

生物安全三级实验室生物安全风险点的识别与控制?

BSL-3实验室须经国家生物安全认可和严格的实验活动审批才能开展相应活动,是国家(地区)开展高致病性病原微生物实验活动的重要技术支撑平台。实验室生物安全风险点易发生在样本及菌毒种的接收、传递和保存,动物实验,污水以及固体废弃物处理等环节,而实验室的意外事件(电力故障、火灾、积水等)与生物安保漏洞也会造成重大的生物安全事故。提示应准确地识别风险并通过人员日常监管、标准操作规程、两级屏障设施和个人防护装备等系列措施对生物安全风险点予以严格控制。     

Effects of the Ratio of n-6:n-3 Polyunsaturated Fatty Acids in the Maternal Diet on Immunoglobulins, Fatty Acid Composition, and Performance of Lactating Sows and Suckling Piglets under Conditions of Low Maternal Energy Intake

This experiment was conducted to investigate the effects of different ratios of n-6:n-3 polyunsaturated fatty acids (PUFA) in sow diets on the performance of lactating sows and their piglets at low digestible energy intakes.Twenty-one pregnant Landrace sows were assigned to one of three diets from day 108 of gestation until weaning (26 to 29 d),containing n-6∶n-3 PUFA ratios of 3∶1,8∶1 and 11∶1.The effects on sow and litter production traits and on sow body condition were examined.There were no differences among treatments in daily feed intakes or in changes in sow weight and back-fat levels during lactation.Litter size,litter weight at birth and weaning and litter average daily weight gain were also unaffected by treatment.As expected,large differences were observed in n-6 and n-3 fatty acids in the colostrum and plasma of sows and piglets (P ＜ 0.01 ).The ratio of n-6∶n-3 PUFA the diet was positively correlated with those in colostrum,sow plasma and piglet plasma ( R2 =0.55,0.80 and 0.80,respectively).Sow plasma insulin and IGF-I levels at weaning were unaffected by the treatments.Plasma leptin ( P ＜ 0.05) concentrations were increased in sows fed the diet with a n-6∶ n-3 ratio of 8∶1.Immunoglobulin concentrations in colostrum were not altered by dietary treatment.Plasma IgG concentrations at d 14 were highest in piglets from sows fed the 8∶1 ratio of n-6∶n-3.Furthermore,this group had the highest IgA concentrations at day 21 of lactation compared with the other two groups ( P ＜ 0.05 ).In summary,our study demonstrated that at low digestible energy levels,altering the ratio of n-6∶n-3 PUFA in the diets for lactating sows affected immune components and the fatty acid composition of lactating sows and their piglets.Further studies are needed to examine whether higher levels of fat supplementation than those used in the present study ( 1.5％ ) can successfully enhance performance.     

牛初乳sIgA等电点的测定

采用等电聚焦电泳法测定牛初乳sIgA的等电点。牛初乳sIgA的pI为5．02—5．67,比相关报道更加精确。回归方程：y=0．7543x＋3．1635,相关系数r=0．9989,表明线性关系良好,为牛初乳sIgA的离子交换层析制备条件的选择提供了可靠的理论依据。     

广东省蚕学会茧丝与蚕桑资源综合利用学组到粤西蚕区进行调研

2001年11月24～27日,广东省蚕学会茧丝与蚕桑资源综合利用学组组织广东省农科院、广东省农科院蚕业研究所、华南农业大学蚕丝科学系、广东省丝绸集团公司、广东省丝绸集团蚕种繁殖试验所等单位的代表,到我省雷州市和化州市等粤     

刈割对羊草和全叶马兰生长与种间关系的影响

研究刈割对羊草(Leymus chinensis)和全叶马兰(Kalimeris integrifolia)生长与种间关系的影响。结果表明:刈割强度越大,相对生长速率越大;留茬4.8和12cm之间差异显著;刈割改变植物间相互作用的方向,削弱种间关系在群落中的重要性;刈割对羊草生长的影响较大。     

云贵川三省部分地区警犬狂犬病血清中和抗体调查

为评价警犬的狂犬病免疫效果,了解影响免疫效果的因素,2017年在云南、贵州、四川三省部分地区免疫狂犬病疫苗的警犬中随机抽样,采用荧光抗体病毒中和试验(FAVN),检测血清中狂犬病病毒中和抗体效价。结果显示,共抽检187只警犬,中和抗体阳性127只(≥0.50 IU/m L),总体免疫合格率为67.91%,其中云南、贵州、四川3省的免疫合格率分别为57.14%、68.33%、76.06%。结果分析表明,警犬狂犬病免疫合格率与犬的年龄、免疫次数、疫苗类型等因素有关,而与性别和品种无关。建议完善警犬免疫程序,加大血清中和抗体监测力度,对不合格警犬及时加强免疫;对每个影响因素进行连续性分析,以确定影响警犬狂犬病免疫合格率的具体因素。     

不同放牧制度和强度下奶牛生产性能的研究──2.放牧对奶牛产奶量及体增重的影响

本文作者在湖南南山牧场首次研究了不同放牧制度(分区轮牧和连续放牧)和放牧强度(0.467,0.667和0.867ha/头)下在多年生黑麦草/白三叶人工草地上放牧的黑白花奶牛产奶量、乳比重及体重的变化。结果表明:轮牧制奶牛的产奶量显著高于连续放牧制(1632.5vs.1462.3kg/头)。不同放牧强度下,奶牛的产奶量以中等放牧强度最高,轻牧次之,重牧最低,分别为1724.5、1571.1和1331.2kgh头。放牧期间两种放牧制间奶牛平均日增重差异不显著,以轮牧制较高(0.224vs.0.205kg/头·天);三种放牧强度间奶牛的平均日增重差异也不显著,由大到小依次为B3>B2>B1,分别为0.242、0.225和0.176kg/头·天。不同放牧处理对牛奶比重影响不大。这说明在本试验条件下,放牧制度和放牧强度对奶牛平均日增重增加影响不显著,但对奶牛的产奶量有显著的影响。