全文获取类型
收费全文 | 13525篇 |
免费 | 908篇 |
国内免费 | 254篇 |
专业分类
林业 | 1428篇 |
农学 | 756篇 |
基础科学 | 199篇 |
2972篇 | |
综合类 | 1418篇 |
农作物 | 592篇 |
水产渔业 | 842篇 |
畜牧兽医 | 4878篇 |
园艺 | 371篇 |
植物保护 | 1231篇 |
出版年
2023年 | 119篇 |
2022年 | 172篇 |
2021年 | 324篇 |
2020年 | 313篇 |
2019年 | 206篇 |
2018年 | 603篇 |
2017年 | 609篇 |
2016年 | 532篇 |
2015年 | 483篇 |
2014年 | 529篇 |
2013年 | 746篇 |
2012年 | 1057篇 |
2011年 | 971篇 |
2010年 | 588篇 |
2009年 | 472篇 |
2008年 | 843篇 |
2007年 | 789篇 |
2006年 | 647篇 |
2005年 | 695篇 |
2004年 | 587篇 |
2003年 | 511篇 |
2002年 | 418篇 |
2001年 | 320篇 |
2000年 | 299篇 |
1999年 | 274篇 |
1998年 | 115篇 |
1997年 | 97篇 |
1996年 | 79篇 |
1995年 | 91篇 |
1994年 | 67篇 |
1993年 | 65篇 |
1992年 | 96篇 |
1991年 | 87篇 |
1990年 | 89篇 |
1989年 | 75篇 |
1988年 | 64篇 |
1987年 | 49篇 |
1986年 | 50篇 |
1985年 | 50篇 |
1984年 | 44篇 |
1983年 | 34篇 |
1979年 | 36篇 |
1974年 | 26篇 |
1973年 | 21篇 |
1972年 | 22篇 |
1971年 | 23篇 |
1970年 | 27篇 |
1969年 | 25篇 |
1968年 | 25篇 |
1967年 | 20篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
991.
Senocq D Dupont D Rolet-Répécaud O Faurie F Levieux D 《Journal of agricultural and food chemistry》2001,49(3):1571-1577
To investigate plasmin activity in cheese, we produced antibodies to bovine beta-casein with controlled specificity, suitable as markers of the integrity of the major bonds involved in its initial breakdown. Sixteen rabbits were immunized with synthetic substitutes for six plasmin-sensitive peptides. Antisera raised to the peptides (f20-39), (f40-56), (f94-113), (f184-202), and (f193-209) recognized beta-casein in ACP-ELISA, Western-blot, and biosensor assays. Casein in vitro hydrolysis by plasmin or chymosin reduced the detection of these determinants in ACP-ELISA, in agreement with the enzymatic sensitivity of bonds included within the binding sites, or in their neighborhood. Antiserum to (f20-39) in particular allowed the specific detection of plasmin cleavage at the bond generating gamma1-CN. Antisera to C-terminus preferentially detected the cleavage by chymosin. Immunoassays using these antibodies would allow in situ monitoring of significant proteolysis events without bias originated in the secondary degradation of the released peptides. 相似文献
992.
The oxidation process of 13 edible oil samples with different proportions of oleic, linoleic, and linolenic acyl groups has been studied using Fourier transform infrared spectroscopy. The oxidation experiments were carried out by heating the samples in a convection oven at 70 degrees C. Duplicate spectra were recorded from a film of pure oil between two disks of KBr for each sample every day during the course of the oxidation, and frequency data of each band of the spectrum were collected automatically. Changes in the values of the frequency of most of the bands of the spectra were observed. The shiftings of the frequency value of specific bands allowed one to distinguish between the different stages of the oxidation process and to establish the oxidation degree of each oil sample. This methodology could be useful to evaluate the oxidative stability of edible oils in a simple, fast, and accurate way. 相似文献
993.
Joaquin Gutiérrez Hugo Travieso M. Aurora Pubillones 《Water, air, and soil pollution》1993,68(3-4):315-323
A method has been developed that uses a mixed solution of NaOH, NaCl and cysteine to digest fish. Inorganic Hg and MgHg were determined by cold vapor atomic absorption (CVAAS) using a selective reduction step with SnCl2 and SnCL2-solutions. Ethyl alcohol was satisfactorily used to prevent excessive foaming. Two optimum digestion procedures were established: (1) using a dry block heater at 90 °C for 1 hr and (2) overnight digestion at 25 to 30 °C. Differences between both were not significant (95%) in the range of 30 to 500 ng g?1 Hg (II) and MeHg in fish. Reduction of the Hg signal due to matrix effects in different fish species was evaluated (95 to 60%). Oily fish, shrimp and lobsters gave the highest reduction. Internal calibration by addition of Hg standards are strongly recommended when exact determinations are needed. The detection limit obtained was near 10 ng g?1. Signals were linear in the concentration range studied (30 to 500 ng g?1). Accuracy of analyses was in the order of 5% or better, with a relative standard deviation of 5%. The method could be applied to other environmental matrices: hair, urine and blood, with some modifications. 相似文献
994.
R Farré M J Lagarda R Montoro 《Journal of the Association of Official Analytical Chemists》1986,69(5):876-879
A method is reported for determination of chromium in foodstuffs. Organic matter is digested with nitric acid, followed by oxidation to Cr(VI) and extraction with methyl isobutyl ketone (MIBK) after HCl addition. Chromium determinations are performed by flame absorption spectroscopy. Absence of interferences is verified and recovery tests are performed on food samples. Quantitation limit (3.8 ng/mL), accuracy (NBS Standard Reference Material 1,573 Tomato Leaves, 4,500 +/- 500 ng/g, found 3,860 +/- 409 ng/g), and precision (CV for vegetable matrix = 9.05%, CV for animal matrix = 14.95%) of the procedure are evaluated. 相似文献
995.
Controlled release of carbofuran from an alginate-bentonite formulation: water release kinetics and soil mobility 总被引:4,自引:0,他引:4
Fernández-Pérez M Villafranca-Sánchez M González-Pradas E Martinez-López F Flores-Céspedes F 《Journal of agricultural and food chemistry》2000,48(3):938-943
The insecticide-nematicide carbofuran was incorporated in alginate-based granules to obtain controlled-release (CR) properties. The basic formulation [sodium alginate (1.61%)-carbofuran (0. 59%)-water] was modified by addition of sorbents. The effect on carbofuran release rate, caused by the incorporation of natural and acid-treated bentonite (0.5 and 1.0 M H(2)SO(4)) in alginate formulation, was studied by immersion of the granules in water under shaking. The time taken for 50% of the active ingredient to be released into water, t(50), was longer for those formulations containing natural bentonite (6.1 h) or acid-treated bentonite (9.0 and 11.7 h for 0.5 and 1.0 M H(2)SO(4) treatments, respectively) than for the preparation without bentonite (4.7 h). It appears from the results that the release of carbofuran from the various formulations is controlled by a diffusion mechanism according to the n values obtained, which were close to 0.5 in all cases. The mobility of carbofuran from alginate-based CR formulations was investigated by using soil columns packed with a clay soil (53% clay and 0.08% organic matter). Two alginate-based CR formulations containing natural bentonite or acid-treated bentonite (0.5 M H(2)SO(4)) were compared to technical grade carbofuran. The use of alginate-based CR formulations resulted in a reduction of the leached amount of carbofuran compared with the total amount of pesticide leached using the technical product (50 and 75% for CR granules containing natural and acid-treated bentonite, respectively). Alginate-bentonite CR formulations might be efficient systems for reducing carbofuran leaching in clay soils, which would reduce the risk of groundwater pollution. 相似文献
996.
Miralbés C 《Journal of agricultural and food chemistry》2003,51(21):6335-6339
Moisture, protein, wet gluten, dry gluten, and alveograph parameters (W, P, and P/L) of whole wheat grown in different countries around the world were analyzed using near infrared (NIR) transmittance spectroscopy. Modified partial least squares on NIR spectra (850-1048.2 nm) were developed for each constituent or physical property. The best models were obtained for protein, moisture, wet gluten, and dry gluten with r(2) = 0.99, 0.99, 0.95, and 0.96, respectively. Initial alveograph NIR models proposed for all wheat samples did not perform well. However, when wheat samples were divided in two groups depending on W (deformation energy) values, NIR models were highly improved, showing enough prediction accuracy for screening wheat at the receiving stage at mills or elevators. 相似文献
997.
998.
Marcos Gallo Alfredo Trento Ana Alvarez Horacio Beldoménico Darío Campagnoli 《Water, air, and soil pollution》2006,174(1-4):367-384
The objective of this work is to analyze Fe, Mn, Cr, Cd, Cu, Pb, and Zn distributions in the water column in terms of total, dissolved, and particulate concentrations in the Salado River (Santa Fe – Argentina); their relationship with total, dissolved and suspended solids; and the impact of two pollution sources on the watercourse: a stream that collects domestic and industrial wastewater discharges of the region and a direct discharge that releases urban and industrial effluents to the river. Water samples obtained from 5 surveys were analyzed. The Salado River transported a load of heavy metals that ranged from 700 g/s to 2200 g/s of Fe, 20 g/s to 60 g/s of Mn and 1 g/s to 3 g/s of Cr. Results show that most metals were found in particulate form, that the metal input from the direct discharge may be significant at low water levels, whereas a lesser impact was produced by the stream, and that both pollution sources were negligible at high water levels. 相似文献
999.
Oxidation of the flavonol quercetin by polyphenol oxidase 总被引:5,自引:0,他引:5
Because direct oxidation of flavonols by polyphenol oxidase (PPO) has not previously been reported and, given the importance of flavonols, the ability of broad bean seed PPO to oxidize the flavonol quercetin was studied. The reaction was followed by recording spectral changes with time. Maximal spectral changes were observed at 291 nm (increase) and at 372 nm (decrease). The presence of two isosbectic points (at 272 and 342 nm) suggested the formation of only one absorbent product. These spectral changes were not observed in the absence of PPO. The oxidation rate, which varied with pH, was highest at pH 5.0. The following kinetic parameters were also determined: V(m) = 11 microM/min, K(m) = 646 microM, V(m)/K(m) = 17 x 10(-)(2) min(-)(1). Flavonol oxidation was efficiently inhibited (K(I) = 3.5 microM) by specific PPO inhibitors such as 4-hexylresorcinol. The results obtained showed that quercetin oxidation was strictly dependent on the presence of PPO. 相似文献
1000.
Jér?me?MathieuEmail author Jean-Pierre?Rossi Michel?Grimaldi Philippe?Mora Patrick?Lavelle Corinne?Rouland 《Biology and Fertility of Soils》2004,40(5):300-305
The influence of three spatially hierarchical factors upon soil macrofauna biodiversity was studied in four pasture plots in eastern Amazonia. The first factor was the local depth of the soil. The second factor was the ground cover type on the soil samples (bare ground, grass tufts, dead trees lying on the ground). The third factor was the dimensions of the grass tufts sampled (size and shape). The effect of each factor upon the morphospecies richness and density of total soil macrofauna was analysed. Detailed results are given for earthworms, termites, ants, beetles and spiders. All factors significantly affected the morphospecies richness and/or density of the soil macrofauna. The type of ground cover had the strongest influence, affecting the total richness and density of the soil macrofauna and of almost all the groups represented. The soil depth affected only the density of the termites and the global morphospecies richness. Interactions between soil depth and ground cover type affected the total macrofauna morphospecies richness and the density of the earthworms. The dimensions of the grass tuft influenced the global morphospecies richness, the morphospecies richness of the ants and the density of the spiders. 相似文献