商洛市平菇栽培及病虫害防治关键技术

商洛市作为我国平菇栽培的重要产区之一,拥有超过50年的平菇人工栽培历史,商洛地区平菇产业不仅在农业经济方面取得显著成就,而且深受市场和消费者喜爱。本文系统总结了近年来商洛市平菇的栽培技术及病虫害防治策略。详细介绍了商洛市平菇目前主推的栽培的季节及品种选择、栽培配方、制袋、养菌管理、出菇管理、采收等内容,分析了平菇的常见病虫害,并提出了相应的防治措施。这项研究为商洛地区平菇产业的发展壮大提供了技术支持。     

Identification of a conservative site in the African swine fever virus p54 protein and its preliminary application in a serological assay

BackgroundASF was first reported in Kenya in 1910 in 1921. In China, ASF spread to 31 provinces including Henan and Jiangsu within six months after it was first reported on August 3, 2018. The epidemic almost affected the whole China, causing direct economic losses of tens of billions of yuan. Cause great loss to our pig industry. As ELISA is cheap and easy to operate, OIE regards it as the preferred serological method for ASF detection. P54 protein has good antigenicity and is an ideal antigen for detection.ObjectiveTo identify a conservative site in the African swine fever virus (ASFV) p54 protein and perform a Cloth-enzyme-linked immunosorbent assay (ELISA) for detecting the ASFV antibody in order to reduce risks posed by using the live virus in diagnostic assays.MethodWe used bioinformatics methods to predict the antigen epitope of the ASFV p54 protein in combination with the antigenic index and artificially synthesized the predicted antigen epitope peptides. Using ASFV-positive serum and specific monoclonal antibodies (mAbs), we performed indirect ELISA and blocking ELISA to verify the immunological properties of the predicted epitope polypeptide.ResultsThe results of our prediction revealed that the possible antigen epitope regions were A23–29, A36–45, A72–94, A114–120, A124–130, and A137–150. The indirect ELISA showed that the peptides A23–29, A36–45, A72–94, A114–120, and A137–150 have good antigenicity. Moreover, the A36–45 polypeptide can react specifically with the mAb secreted by hybridoma cells, and its binding site contains a minimum number of essential amino acids in the sequence 37DIQFINPY44.ConclusionsOur study confirmed a conservative antigenic site in the ASFV p54 protein and its amino acid sequence. A competitive ELISA method for detecting ASFV antibodies was established based on recombinant p54 and matching mAb. Moreover, testing the protein sequence alignment verified that the method can theoretically detect antibodies produced by pigs affected by nearly all ASFVs worldwide.     

樱桃小茎尖培养后的早熟变异与RAPD鉴定

樱桃品种Ｘｅｓｐｈｙｅ（Ｐｒｕｎｕｓ　ａｖｉｕｍ）的休眠芽通过小茎尖分生组织培养后,发现一株组培苗结果后成熟期明显早于Ｘｅｓｐｈｙｅ及同批其它试管苗,该株经无性繁殖试栽后早熟性状稳定,并且综合性状良好,具栽培价值,暂定名“早丹”。经ＲＡＰＤ技术鉴定,该早熟变异株的ＤＮＡ指纹图谱与原Ｘｅｓｐｈｙｅ品种不同,早熟变异株比原 Ｘｅｓｐｈｙｅ品种多扩增一条 ＯＰＵ０３－９５０ ＤＮＡ谱带,说明该株系发生了ＤＮＡ的变异。     

聚类分析在我国果树研究中的应用及问题分析

对近１０年来聚类分析在我国果树研究中的应用情况进行了简要总结。涉及十余种果树及系统聚类和模糊聚类两种聚类分析方法。文中对有关问题进行了分析和讨论。     

6.5%硫菌·霉威粉尘剂防治番茄灰霉病田间药效试验

灰霉病是保护地番茄最重要的病害之一 ,一般的喷雾防治常因增加温室湿度而防效不佳。粉尘法是适合于温室病害防治的新技术 ,具有操作简便、不增加温室湿度 ,防效高等优点。 1999年笔者进行了6.5%硫菌·霉威粉尘剂防治番茄灰霉病的药效试验 ,现将试验结果总结如下。1　试验材料与方法1.1　材料　供试药剂为 6.5%硫菌·霉威粉尘剂 ,由丹东市农药二厂提供 ;65%硫菌·霉威可湿性粉剂 ,为江苏新沂农药厂产品。供试作物为佳粉 15号番茄。1.2　方法　试验田设在沈阳市东陵区前进乡温室番茄田 ,历年番茄灰霉病发生较重。土壤肥力中等 ,ｐＨ 6.9。试…     

寒地山区枣引种栽培综合技术研究

发展贫困地区经济,解决贫困农民温饱问题,是我国当前工作的重点之一.经调查,宁城县部分乡镇分布着几万公顷野生酸枣资源,仅布日嘎苏台乡就有133hm2,基本没有经济效益,多被农民砍伐,烧柴或架篱笆用.如对这一资源改造利用,可产生良好的经济效益和社会效益.本项目以当地资源为依托,引进枣树优良品种,进行栽培综合技术研究,旨在发展贫困山区经济,为农民迅速致富探索成功经验.     

李属种质资源的抗寒性鉴定

为了给品种区化和资源的育种利用提供理论依据,于１９９５－１９９６年在辽宁熊岳作者用外渗电导法、揭变观察法和恢复生长法测定了属于中国李、欧洲李、杏李、加拿大李、美洲李、包苏里李、樱桃李和李、杏杂种的３６个代表品种和类型的抗寒性。结果表明;（１）李的抗寒性表现出丰富的多样发表一多数李品种对低温的适应能力较强;（２）起 的生态２条件与品种的抗寒性密切相关;（３）李几个主要种的抗寒性顺序为：乌苏里李〉美洲     

6-3-3并联机构逆动力学分析与仿真

建立了6自由度6-3-3并联机构逆运动学模型,基于刚体动力学牛顿-欧拉法推导出了该机构逆动力学方程组,并用矩阵QR分解方法求解了该动力学方程组,给出了逆动力学仿真程序开发思路并通过Matlab软件加以实现,通过空载和有载荷作用条件下的算例仿真和分析比较,验证了该动力学模型的正确性.     

中国上古社会伦理政治的演进

中国上古社会政治文化随着夏商周神权政治统治的式微和伦理政治实践的推行,到春秋战国时期人文礼治的兴起,尤其是原始儒家确立起以伦理主导政治与以政治实践伦理的伦理政治模式,呈现出由多元走向一元的总体趋势。