Experimental study of renal radiofrequency ablation guided by high-frequency electrical stimulation

AIM: To evaluate the guiding effect of high-frequency stimulation (HFS) on renal denervation (RDN), and to compare the similarities and differences of blood pressure changes at the time of electrical stimulation and radiofrequency ablation. METHODS: A total of 6 Kunming dogs were included in this study. Renal artery abnormalities were excluded by angiography. High-frequency stimulation and radiofrequency ablation were performed at the same sites from distal to proximal segments of the renal artery. Invasive blood pressure (BP) was recorded during the whole procedure. The change of the blood pressure was analyzed. HE and Masson staining was adopted to detect the structural changes in the wall of the renal artery and surrounding tissues. The immumohistochemical staining for tyrosine hydroxylase (TH) was used to observe the renal nerve damage after ablation. RESULTS: Electrical stimulation and radiofrequency ablation were delivered in a total of 50 sites. The BP increasing response was induced at 34% sites (n=17), while the rest sites (66%, n=33) had no response. Compared with the baseline, HFS caused the increases in systolic BP of (0.34±3.38), (0.41±3.04), (10.47±5.73), (13.27±3.63), (10.17±1.87) and (0.78±1.87) mmHg in 6 serial 20 s time segments during 120 s of HFS at positive BP response sites. Similarly, the increases in systolic BP by (-0.88±3.44), (-1.64±3.47), (13.17±3.12), (12.82±3.21), (9.50±2.68) and (-6.09±2.21) mmHg were observed during 120 s of ablation procedure at the same sites in 6 serial 20 s time segments. At non-responding sites, HFS and ablation failed to cause a significant increase in systolic BP. The mean area of nerves in the response sites was (0.51±0.28) mm², whereas that in non-response sites was (0.09±0.06) mm² (P<0.01). The average absorbance values of TH in renal nerves at ablation and non-ablation sites were 0.031±0.015 and 0.085±0.018 (P<0.01), respectively. CONCLUSION: Renal sympathetic nerves can be effectively mapped by HFS. Radiofrequency ablation guided by HFS is valid to injure sympathetic nerves around renal artery.     

Establishment of patient-derived esophageal squamous-cell carcinoma xenograft in mice and characteristics of signaling pathways related to proliferation in SCID mice

AIM: To establish and characterize the patient-derived esophageal squamous-cell carcinoma xenograft (PDECX) in mice. METHODS: The samples of human esophageal cancer were grafted into severe combined immunodeficient (SCID) mice. The xenografts were transferred to SCID mice when the first passage of xenografts grew up. The growth of tumors in the first, second and third passages was observed. HE staining was performed. The expression of CK5/6, p63 and p40 in the patient samples, and the first and third passages of the xenografts were detected by immunohistochemical analysis. The expression of mTOR, p-mTOR, p70S6K, p-p70S6K, Akt1, p-Akt (Ser473), Erk1/2 and p-Erk1/2 were determined by Western blot.RESULTS: The PDECX was successfully established. The positive expression of CK5/6, p63 and p40 in the xenografts was consistent with that in the patients' samples. The levels of phosphorylated and total proteins of proliferation-related signaling pathways were different in the xenografts from different patients.CONCLUSION: The PDECX model adequately reflects the tumal heterogeneity that is observed in the patients.     

黄河首曲流域草地生态与自然环境退化成因及对策研究

近50年来黄河首曲流域草场退化、沙化,水土流失,生物多样性减少,生态系统功能弱化.分析了黄河首曲流域气候、生态观测资料及统计资料,结果表明:造成生态与自然环境退化的主要原因是气候变化和环境蠕变.黄河首曲大部分区域降水量年际变化呈下降趋势,气温年际变化呈上升趋势,增温速度均大于全国增温速度.草地年干燥指数呈显著上升趋势,20世纪80年代末至2004年明显趋于干旱化,气候变化是草地生态退化的自然诱发因素.超载过牧、滥采乱挖、人为破坏、生物链失衡等环境蠕变是造成生态退化的人为因素.二者共同作用导致黄河首曲流域草地与湿地生态与自然环境退化.控制放牧、防止滥采乱挖、建立自然生态保护区是维护该区域生态系统平衡的措施.     

Bt与EoNPV合理混配的筛选程序

苏云金杆菌（Bt）和核型多角体病毒（NPV）是活体微生物杀虫剂中应用最多的两种类型。为提高杀虫刹的防治效果,人们常将它们混合使用,如Bt与茶尺蠖核型多角体病毒（EoNPV）的混用。实践表明,混用后与单用相比,杀虫速度和广谱性均比NPV提高;其药效的均衡性和持久性也比它们分别单用有所改善。为了使这些功能特性标准化和技术手段规范化,我们拟进行Bt与EoNPV合理混配筛选程序的研究,采用时间效率（如联合时效系数）和剂量效率（如共毒系数）等方面的多种量化指标,试图对混剂理想配比作出综合判断。     

大葱伴生甜瓜栽培对根际土壤酶活性和细菌群落的影响

为解决设施栽培土壤恶化引起的甜瓜产量持续下降问题,本研究以大葱与甜瓜进行伴生栽培试验,基于传统土壤农化、土壤酶分析和现代高通量测序技术分析,探究伴生对根际土壤农化、土壤酶、土壤细菌群落结构的影响.结果表明,伴生栽培甜瓜根际土壤细菌数量低于甜瓜单作处理,但差异不显著,而伴生处理的Shannon指数显著高于甜瓜单作处理,表...     

浅析吉林省玉米新品种知识产权保护战略

在统计分析吉林省23年玉米品种审定相关数据的基础上,比较分析了玉米育种研究专利和品种权保护的有关状况,探讨吉林省玉米育种知识产权保护战略的相关问题。     

水氮耦合对无芒雀麦产量及氮磷钾化学计量特征的影响

[目的]合理的水分和养分供给是保障人工草地高效可持续生产的有效措施.探究不同灌溉量和施氮量及其交互作用对牧草产量及养分含量的影响,有助于深入了解牧草生长对草地生态系统的响应.[方法]以人工种植无芒雀麦(Bromus inermis)为研究对象进行田间试验.采用喷灌方法,在无芒雀麦拔节期按照田间持水量(θf)范围设置4个...     

东江中上游4树种水势日变化特征及其与环境因子的关系

在东江中上游,以6a生树种木荷、红锥、火力楠和藜蒴为材料,采用PSYPRO露点水势仪对4树种在旱季与雨季的土水势、根水势、茎水势和叶水势日变化过程进行了测定,探讨了4树种在自然生境下的水势梯度变异及叶水势与气象因子的关系。结果表明,叶水势日变化均呈"单峰"型曲线,日均值表现为木荷>火力楠>红锥>藜蒴,旱季叶水势明显低于雨季;叶水势与大气温度(Ta)和光合有效辐射(PAR)呈极显著负相关(P<0.01),而与空气相对湿度(RH)和大气水势(Фa)呈极显著正相关(P<0.01),气象因子对东江流域主要造林树种叶水势直接作用由大到小结果如下:火力楠和藜蒴(Ta>RH>Фa>PAR),红锥(RH>Фa>Ta>PAR),木荷(Ta>Фa>RH>PAR)。