日粮中添加整粒小麦对肉鸡生产性能、胴体指标和消化器官的影响

试验选用1日龄科宝500商品肉仔鸡2 480只,以研究在肉鸡日粮中使用整粒小麦对肉鸡生产性能、胴体指标和消化器官的影响.将试验鸡随机分为2个处理,每个处理4个重复,每栏饲养310只.肉鸡1～21日龄,对照组和试验组均饲喂相同的破碎料.22～42日龄,对照组饲喂颗粒饲料;试验组在22～35日龄和36～42日龄分别将颗粒饲料中10%和15%的粉碎小麦用整粒小麦代替,营养水平与对照组相同.试验结果表明:在肉鸡后期日粮中使用整粒小麦对生产性能影响不显著(P＞0.05);除母鸡腿肉率显著降低外(P＜0.05),饲喂整粒小麦对公鸡腿肉率、公母鸡全净膛率、胸肉率和腹脂率均影响不显著(P＞0.05);饲喂整粒小麦可以促进肌胃的发育,显著提高肌胃的相对重量(P＜0.05),对腺胃的相对重量影响不显著(P＞0.05).     

进一步解放思想 繁荣高校哲学社会科学

从江泽民同志“四个同样重要”理论出发，结合云南农业大学大学精神建设、社会科学学科建设、学报社科版建设以及校园文化建设等方面的基本情况，论证了进一步解放思想对于繁荣高校哲学社会科学的重要意义。     

实行借书逾期罚款制度涉嫌侵权

作者根据国家的有关法律法规 ,提出了图书馆实行借书逾期罚款制度无法律依据 ,超越了有关规定 ,是一种侵权行为 ;同时对做好读者服务工作 ,加强图书馆规章制度的宣传教育方面谈了自己的看法。     

狂犬病毒RT-PCR检测方法的建立

根据狂犬病毒保守序列设计引物,利用反转录聚合酶链式反应(RT-PCR)建立了一种检测狂犬病毒的方法,以期实现对严重危及人和动物生命安全的狂犬病毒直接检测。     

杨树苗圃地化学除草效果试验

杨树苗期杂草危害严重,以农作物常用除草剂防除杂草,对苗木生长有促进作用。通过试验结果表明,除草剂草甘膦、百草枯等在常规用量时对杨树苗期生长效果显著、安全,用药后12d禾本科、阔叶杂草死亡率达100%,省时省工,成本低,见效快。     

云南撒坝猪H-FABP基因多态性及与部分生产性能的关系

采用PCR-RFLP技术检测H-FABP基因5′上游区1125~1817区段HinfⅠ酶切位点在撒坝猪群体中的多态性分布,并采用最小二乘分析模型初步统计分析检测位点多态性与部分生产性能的相关性。结果表明,H-FABP基因等位基因H及基因型Hh在群体中占优势,频率分别为0.6022和0.4194;H-FABP基因在群体中处于Hardy-Weinberg平衡状态;H-FABP基因检测位点基因型间在生长和繁殖性状上不存在不利影响。     

鹅副粘病毒感染鸡试验

本室分离的鹅副粘病毒是一侏对鹅具有料强致病毒，它也能实验感染雏鸡，且雏鸡感染后发病率均为１００％，死亡率５０％以上。人工感染的鸡其症状和病理变化均与鹅自然发病的病变相类似。从感染途径看，注射接触（混养）均能感染发病，并且在感染发病及死亡时间上也与鹅感染情况相似，因此该病毒感染鹅同时又能实验感染雏鸡，而且传播之迅速，危害之严重必须引起养禽户的重视。     

Study on the Anti-Escherichia coli Activity and Mechanism of Water Extract of Radix isatidis Powder

In order to explore the antibacterial activity and mechanism of the Radix isatidis powder water extract,the effects of the Radix isatidis powder water extract on the morphology and structure of Escherichia coli (E.coli) were tested by scanning electron microscopy and transmission electron microscopy.The effects of the Radix isatidis powder water extract on the conductivity and total leakage rate of E.coli and the content of alkaline phosphatase in the culture medium of the content of protein,DNA and RNA were stained by DAPI to detect the effect of the Radix isatidis powder water extract on the nucleic acid of E.coli,and the effect of the Radix isatidis powder water extract on the metabolism of E.coli in vivo,in vitro,ALT,AST,pyruvic acid and ATP.The results showed that after the Radix isatidis powder water extract acted on E.coli for 10 h,it was observed by SEM that the bacteria appeared to overflow and shrink,the length of the bacteria became shorter obviously,many residues were formed due to breakage,some of them were sunken in the middle and deformed.Under TEM,it was observed that the boundary of the cell wall of E.coli was unclear,the wall membrane was zigzag,deformed and some of the bacteria were broken.The protein content outside the cell was significantly different from that in the blank control group from 8 h (P<0.01), and that in the cell from 4 h (P<0.01). DNA content had no significant difference with blank control group before 12 h (P>0.05), but had significant difference with blank control group from 16 h (P<0.01); RNA content began to decrease at 8 h, and was significantly different from blank control group at 12 h (P<0.05), and was extremely significant from 16 h (P<0.01). There was no significant difference between ALT and AST (P>0.05). The pyruvate content in culture medium and bacteria was higher than that in blank control group, and the difference was very significant from 4 h (P<0.01). The ATP content in the culture medium was significantly different from that in the blank control group (P<0.01), and the ATP content in the cell was significantly different from that in the blank control group from 4 h (P<0.01).In conclusion,the Radix isatidis powder water extract could inhibit the synthesis and metabolism of bacterial genetic material and the content of pyruvate and ATP by destroying the integrity of cell wall and cell membrane.