芩连液与白虎汤对气分证家兔免疫调节及抗氧化活性的影响比较

为比较自拟方芩连液和白虎汤对气分证的疗效差别,以静脉注射脂多糖(LPS)制作气分证家兔模型,在两种药物治疗前后分别检测了血清免疫球蛋白IgG、IgM和IgA的含量变化,以及血清超氧化物歧化酶(SOD)、丙二醛(MDA)和总抗氧化能力(T-AOC)的变化。结果显示,白虎汤对气分证家兔升高了的血清免疫球蛋白和T-AOC的含量变化无显著影响,但能使血清SOD下降,MDA上升;芩连液可使得IgM和IgG含量下降,SOD上升,MDA无显著变化,而T-AOC含量下降。结果表明,白虎汤对气分证家兔免疫调节和总抗氧化能力的保持优于芩连液,但对SOD和MDA调节不及芩连液。     

T4噬菌体蛋白的分子改造及其应用前景

T4噬菌体是一种病毒,其基因、结构和生物学特性已清晰,利用各种分子操作技术可以对其进行进一步改造。利用位点特异性诱变,研究T4噬菌体某些基本蛋白的功能;普遍用来进行噬菌体改造的噬菌体展示技术已经成功在T4衣壳平台上展示目的抗原;位点特异性诱变及噬菌体展示技术都可以用于改造噬菌体使其与哺乳动物细胞发生相互作用,也能获得除细菌宿主之外的可被噬菌体感染的细胞;还可在不断发展的噬菌体治疗研究中得到应用。这些噬菌体的改造方法都将会促进疫苗技术、噬菌体疗法和其他生物及医学科学分支的发展。论文对T4噬菌体的蛋白结构、噬菌体展示技术及相关的噬菌体治疗等内容进行了综述。     

饲料原料与瘤胃培养残渣(8、12、16小时)氨基酸组成差异的研究

选用3头装有瘤胃瘘管的改良阉牛 ,利用尼龙袋技术对不同饲料降解后残渣的氨基酸(AA)组成与原料AA组成的差异进行了研究。结果表明 ,与精料氨基酸浓度相比在降解后有上升趋势(P<0.05)的氨基酸有Ala、Phe、Val、Ile和Leu(其中后3种氨基酸为支链氨基酸) ;在降解后有下降趋势(P<0.05)的氨基酸有Gly、Arg、Tys、Lys、Met和Cys。各氨基酸降解前后的浓度存在强相关回归关系。     

细胞永生化的研究进展

永生细胞系具有无限增殖能力,其自我更新能力、增殖分化模式、基因表达调控以及癌症等疾病研究一直以来也是分子细胞生物学领域的研究热点与难点.细胞永生化是指细胞在体外培养的时候,由于自身基因改变或者外界因素刺激,例如细胞周期检查点通路受损、端粒酶的再次激活上调、原癌基因激活等影响,使细胞分裂加快,并突破了自我衰老与凋亡机制,...     

基于猪丁型冠状病毒重组M蛋白间接ELISA抗体检测方法的建立与应用

猪丁型冠状病毒(porcine deltacoronavirus,PDCoV)是近年新发现的一种猪肠道病原体,主要感染仔猪并引起仔猪腹泻甚至死亡,针对该病建立快速、准确的血清学检测方法尤为重要.本研究以PDCoVHNZK-04毒株为模板扩增M基因并将其克隆至原核表达载体pET-32a中,构建了重组质粒pET-32a-P...     

现代畜牧生态学原理与应用

现代生态畜牧是将生态学、生态经济学、系统学及可持续发展理论与方法应用于畜牧业生产而发展起来一个新领域,已成为现代畜牧业生产中实现经济、生态、社会效益稳定协调发展的指导思想。本文就生态学基本原理在现代畜牧业生产中应用及进行生态畜牧业生产中技术进行简要介绍。     

The Effect of Training on Yili Horse 1 600 m Test Results and Heart Rate Variability

【Objective】 The objective of this study was to analyze the effect of different training stages on the performance and heart rate variability(HRV) of Yili horses and to provide data for reference in the conditioning training of 1 600 m distance Yili horses.【Method】 Eight 3-year-old Yili horses (stallions) were selected as test subjects.A 3-month speed specific performance training program was conducted, and a speed test race was organized in the last week of each month of training, and HRV was collected before, immediately after, 0.5 h after, and 1 h after 1 600 m test race.Among them, the time-domain indexes included the mean of all R-R intervals (Mean RR), the standard deviation of all R-R intervals (SDNN), the mean heart rate (Mean HR), the root mean square of the difference between adjacent R-R intervals (RMSSD), the number of adjacent R-R intervals with a difference greater than 50 ms (NN50), and the percentage of adjacent R-R intervals with a difference greater than 50 ms to the total number of heartbeats (pNN50).Frequency domain metrics included very low frequency (VLF), low frequency power (LF), high frequency power (HF) and nonlinear metrics:standard deviation (Y) of all R-R-spacing (SD1), standard deviation (X) of all R-R-spacing (SD2).Consequently, the variability of HRV indexes in horses at different training stages was analyzed.【Result】 The race time in the post training period in 1 600 m speed test race of Yili horses was significantly lower than that in the early training period (P<0.05), and Mean RR, NN50 and pNN50 in the middle and post training periods were significantly lower than that in the early training period (P<0.05).Mean HR in the beginning of training was significantly lower than that in post training (P<0.05).VLF and LF in the end of training were significantly lower than that in the beginning of training (P<0.05).【Conclusion】 Under the conditions of this test, the HRV indexes of 1 600 m test race of Yili horses in different conditioning training stages were analyzed.The outcomes demonstrated that the types of neural activity in Yili horses at the beginning, middle and end of training presented some differences, showing changes of increased sympathetic excitability and decreased parasympathetic excitability, and the horses' athletic performance improved.Therefore, HRV could be considered as an effective tool to evaluate the training load and intensity of Yili horses during training.     

Response of grain yield and related agronomic traits of oilseed flax to diversified cropping rotations北大核心CSCD

This research aimed to identify a suitable planting pattern for oilseed flax production in a dry-farming region. A long-term field experiment was started in 2012 with a 4-year crop rotation cycle,designed to measure the effects on flax crop performance of previous crop,and various patterns of flax planting interval within the four-year rotation,compared with continuous flax cropping. Crop parameters measured included plant height,stem diameter,dry matter accumulation and distribution,and the experiment included six different crop rotation patterns：Flax→flax →flax→flax［（F）FFF］;Flax→wheat→potato→flax［（F）WPF］;Flax→potato→flax→wheat［（F）PFW］;Flax→ flax→wheat→potato［（F）FWP］;Flax→wheat→flax→potato［（F）WFP］and flax→wheat→potato→wheat［（F）WPW］. Results for the ninth year showed significantly increased grain yield（29. 89%-109. 57%）in crop rotation treatments compared with continuous cropping of oilseed flax. The ranking of the six tested rotations for yield was：（F）WPW>（F）FWP>（F）WFP>（F）PFW>（F）WPF>（F）FFF. The grain yield of oilseed flax was significantly affected by previous crop,frequency and years interval of flax cropping and number of years of continuous flax cropping. Yield was increased by 54. 45%-59. 29% under wheat stubble and potato stubble compared with oilseed flax stubble,and increased by 30. 66% and 109. 57%,respectively,under 50% and 25% frequencies,compared with 100% frequency. The grain yield of oilseed flax under two-year continuous cropping was higher by 29. 89% than four-year continuous cropping,and increased with increase in years interval between flax crops. Correlation analysis identified a significantly positive correlation between oilseed flax grain yield and effective capsule number,branch number and 1000-seed weight. The effective capsule number,branch number and 1000-seed weight of oilseed flax under rotation treatment were increased by 35. 88%-108. 91%,15. 47%-46. 19% and 14. 61%-16. 34%,respectively（P<0. 05）,compared with continuous cropping. In addition,the high grain yield of oilseed flax was accompanied by an increase in plant height,stem diameter and dry matter accumulation and these increases were,respectively,5. 11%-42. 24%,2. 77%-39. 92% and 31. 25%-117. 89% under the rotation regimes,compared with continuous cropping. Reduction in the number of years of continuous cropping years,change of crop stubble,decreased flax planting frequency and increased of interval between flax crops also improved flax crop performance. In summary,crop rotation improved the vigor of oilseed flax,resulting in greater plant height and stem diameter,improved dry matter accumulation and distribution,leading to increased branch number,effective capsule number and 1000-seed weight,and increase in the crop yield of oilseed flax. The results indicated that a multiple-crop rotation pattern was an effective way to avoid the yield reduction caused by continuous cropping in oilseed flax. The rotation： Flax→wheat→potato→wheat performed best among those tested and can be recommended as an appropriate cropping rotation for oilseed flax production in the dry region of northwest China. © 2022 Editorial Office of Acta Prataculturae Sinica. All rights reserved.     

鸡新城疫-传染性支气管炎-减蛋综合征三联油乳剂苗的研制及应用

用新城疫病毒（克隆H88株。）、传染性支气管炎病毒（JP株和H52株）和减蛋综合征病毒（H91株）分别接种于鸡胚和鸭胚,并收取其含毒鸡、鸭胚尿囊液,用一定浓度甲醛溶液灭活,按一定比例混匀,以7号白油为佐剂制成鸡新城疫（ND）－传染性支气管炎（IB）－减蛋综合征（EDS76）三联油乳剂灭活苗（三联苗）。三联苗免疫30日龄雏鸡,免疫后7天产生免疫应答,免疫后21天保护率达100％。疫苗在4℃～8℃保存期为12个月,10℃～25℃保存期为6月,该疫苗在全省不同地区鸡群应用400余万羽份,取得满意效果。     

蓝芩注射液体外抑菌试验

大肠杆菌、猪霍乱沙门氏菌、葡萄球菌、猪链球菌是常见病原菌，在不同诱因存在下引起发病，对这类疾病多数采用抗菌药物治疗，细菌易产生耐药性。耐药性的产生又需要加大药量或变换药物品种，这样既增加了治疗的成本，又使得畜产品中的药物残留问题更加突出。许多中药具有抗菌消炎和调节机体免疫的功能，并具有副作用小、残留低、不易产生耐药性等优点。笔者等观察了蓝芩注射液对4种病原微生物的体外抑菌作用，现报道如下。