苜蓿青饲对波尔山羊瘤胃消化代谢的影响

本文旨在研究不同比例的苜蓿青饲对波尔山羊瘤胃消化代谢的影响.选取4只体重为(39.80±1.45)kg的装有永久性瘤胃瘘管的波尔公山羊,采用4×4拉丁方设计,4个处理饲粮精料组成相同,粗料组合分别为:100%切碎花生秧(对照组)、85%切碎花生秧+15%苜蓿鲜草(试验Ⅰ组)、70%切碎花生秧+30%苜蓿鲜草(试验Ⅱ组)...     

牙鲆养殖循环系统中固体废物的去除研究

对自行设计的牙鲆Paralichthys olivaceus循环养殖系统采用斜板式沉淀槽、水力旋流器、泡沫分离器去除固体颗粒物的效果进行了研究。结果表明:该系统实际运行过程中,养殖槽的固体颗粒物含量小于10 mg/L;对养殖水中总悬浮固体颗粒物的去除能力,沉淀槽最强,去除量(干重,下同)为(11.3±3.5)g/d,占69.2%,水力旋流器为(4.8±1.0)g/d,占29.5%,泡沫分离器为(0.2±0.1)g/d,占1.3%;对难沉降颗粒物的去除能力,泡沫分离器较强,去除量为(4.2±1.1)g/d,占53.1%,其中挥发性悬浮固体占93.2%,沉淀槽为(3.6±0.7)g/d,占44.8%,水力旋流器为(0.2±0.0)g/d,占2.1%;牙鲆摄食饲料所产生的固体废物量(干重)约为245.8 g/kg。     

弘扬茶文化促进社会主义和谐社会建设

中国是茶的故乡,是世界上茶历史最悠久、茶资源最丰富、茶文化最深厚的国家.茶在中国始终扮演着社会交往的最佳礼物和媒介,是人与人之间友谊的桥梁.茶文化的本质是"和谐文化",它引导人们与自然和谐相处,启示人们在人与人的关系上,要彼此尊重,加强交流,启发人们感悟身心的统一.大力弘扬茶文化精神,是建设和谐文化的一笔重要资源,是构建和谐社会的重要载体.     

刺槐平茬后的经营管理技术研究

刺槐林木生长到中龄林以上阶段,出现老化衰退现象,经人工平茬后达到萌蘖复壮更新的目的,其经营管理技术极为重要。通过清除伐根、严格管护、林间挖沟、补植等系列经营管理技术,才能达到复壮更新的目的。     

新疆春小麦育成品种遗传演变分析

【目的】研究新疆春小麦育成品种遗传演变规律,提高小麦资源的利用效率,为新疆春小麦品种选育和改良提供参考依据。【方法】以新疆春小麦育成品种为材料,对其主要性状进行综合评价分析,以春小麦90K芯片开展新疆春小麦育成种亲缘关系分析。【结果】新疆春小麦育成种遗传多样性丰富,平均遗传多样性指数2.005,变幅为1.902~2.181。相关性分析结果表明,蛋白质含量、湿面筋含量、穗粒数与育种年代呈极显著正相关。新疆春小麦品种选育主要是通过常规杂交育种、杂交辐射诱变育种、引种3种方式。利用小麦90K芯片将新疆春小麦育成种划分为3个类群,显示了各品种之间的亲缘关系。【结论】新疆春小麦育种遗传基础薄弱、遗传多样性逐渐散失,新疆小麦育种应加强资源收集与利用,扩大育种亲本选择,提高品种变异的丰度和广度,以多抗、高产稳产、优质高效的聚合育种,加快新品种选育进程,提高育种效率。     

能量调控对草莓果实采后衰老过程中活性氧及保护酶的影响

以‘红颜'草莓果实为试材,采用呼吸链解偶联剂2,4-二硝基苯酚(DNP)和外源ATP处理草莓果实,研究抑制和促进能量生成条件对草莓果实衰老过程中活性氧和保护酶的影响。结果表明,在草莓果实采后衰老过程中,DNP处理、ATP处理和对照的脂氧合酶(LOX)活性、过氧化氢(H_2O_2)含量、超氧阴离子(O_2~-)产生速率、丙二醛(MDA)含量和相对电导率均为上升。DNP处理的LOX活性、O_2~-产生速率、MDA含量和相对电导率均高于对照,CAT活性和APX活性基本均低于对照;ATP处理后上述活性氧指标均低于对照,CAT活性和APX活性基本高于对照。以上结果说明,采用外源DNP处理果实使保护酶活性下降,同时加剧了活性氧的上升与积累;外源ATP处理则提高了保护酶活性,从而缓减了活性氧的上升与积累。能量水平和活性氧代谢相互作用影响果实衰老进程,能量调控可以影响活性氧代谢从而影响草莓果实的衰老。     

滨海农区苜蓿栽培技术专家系统构建

本文详细介绍了滨海农区苜蓿栽培技术专家系统构建的规则和过程，并介绍了该系统的特点和使用方法。     

Effect of electroacupuncture on microglia phenotype in injured abducens nerve

AIM: To investigate the effect of electroacupuncture treatment on microglia phenotype in Beagle dogs with abducens nerve injury. METHODS: Healthy adult Beagle dogs were randomly divided into 4 groups:normal control group, sham operation group, injury group and electroacupuncture treatment group, with 5 dogs in each group. In sham operation group, only the isolated nerves were exposed. In injury group, the dogs were restrained when electroacupuncture was performed in electroacupuncture treatment group. In electroacupuncture treatment group, electroacupuncture was performed on the base of dog nerve injury. The experiment was continued for 2 weeks. Western blot was used to detect the expression levels of M1 and M2 markers in the microglia. RESULTS: The expression of inducible nitric oxide synthase (iNOS) in injury group was significantly higher than that in control group (P<0.05). In electroacupuncture treatment group, the expression of iNOS was significantly lower than that in injury group (P<0.05). The expression of interleukin-1β (IL-1β) in injury group and electroacupuncture treatment group was higher than that in control group and that in electroacupuncture treatment group was significantly lower than that in injury group (P<0.05). The expression of arginase in injury group and electroacupuncture treatment group was higher than that in control group (P<0.05), and that in electroacupuncture treatment group was higher than that in injury group (P<0.05). Meanwhile, the expression of brain-derived neurotrophic factor (BDNF) in injury group and electroacupuncture treatment group was increased compared with control group (P<0.05). But the expression of BDNF in electroacupuncture treatment group was higher than that in injury group (P<0.05).CONCLUSION: After abducens nerve injury, electroacupuncture treatment reduces the expression levels of M1 marker proteins in microglia and increases the expression levels of M2 marker proteins.     

毛竹林主要害虫工程治理技术

竹产业是浙江省龙游县最具优势、最有发展潜力的支柱产业。全县现有毛竹(Phyl lostachyspubescensMazelexH.deLehaie)林面积21344hm2,占有林地面积的35 2%,竹业年产值达10亿余元。毛竹主要虫害是该产业发展的最大障碍之一。为寻求安全、有效的治理技术,实现毛竹林主要害虫的可持续控制,自1990年以来,结合竹林生产防护,作者开始对毛竹林主要害虫的综合治理技术进行研究,并于1998年正式立项实施毛竹林主要害虫工程治理。1　工程治理区概况工程治理区毛竹林面积19449.7hm2,占全县毛竹林面积的91 2%,涉及7乡1镇。竹林集中连片,单一纯林占88 7%…     

郑抗系列无籽西瓜品种SSR指纹图谱的构建

为实现无籽西瓜品种的快速、准确鉴定,用23对西瓜核心SSR引物分析了15份无籽西瓜品种的DNA指纹。23对核心引物中有21对具多态性,共扩增出58个基因型,平均2.71个,平均多态性信息量(PIC)为0.36。10对引物在9个品种上具有特征谱带,组合PIC值0.4的8对引物可以将参试的15个无籽西瓜品种一一区分开,并利用这8对引物构建了参试品种的数字指纹图谱。研究表明,西瓜核心SSR引物适合用于构建无籽西瓜品种的DNA指纹,将为无籽西瓜品种真实性鉴定和知识产权保护提供有效的技术依据。